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A Histological and Ultrastructural Study of Variegated Leaf Chimeras of Saintpaulia Ionantha Wendl. and Their Regeneration in Vitro.

Record Type:	Electronic resources : Monograph/item
Title/Author:	A Histological and Ultrastructural Study of Variegated Leaf Chimeras of Saintpaulia Ionantha Wendl. and Their Regeneration in Vitro./
Author:	Norris, Richard Eugene.
Description:	1 online resource (138 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 42-07, Section: B.
Contained By:	Dissertations Abstracts International42-07B.
Subject:	Botany. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=8128916click for full text (PQDT)
ISBN:	9798661616531

A Histological and Ultrastructural Study of Variegated Leaf Chimeras of Saintpaulia Ionantha Wendl. and Their Regeneration in Vitro.
Norris, Richard Eugene.

A Histological and Ultrastructural Study of Variegated Leaf Chimeras of Saintpaulia Ionantha Wendl. and Their Regeneration in Vitro. - 1 online resource (138 pages)

Source: Dissertations Abstracts International, Volume: 42-07, Section: B.

Thesis (Ph.D.)--Texas A&M University, 1981.

Includes bibliographical references

Variegated leaves of three African violet cultivars were studied by light and electron microscopy. This analysis combined with inheritance data revealed the following possible LI, LII, LIII periclinal chimera histogenic organizations: (a) cv. Marge Winters, GGW-type, (b) cv. Bold Dance, GWG-type, (c) cv. Calico Kitten, WWG-type. Electron micrographs of the mutated sections showed plastids that had dilated thylakoids typical of PSI mutants. Leaf and petiole explants of these variegated cultivars were cultured using a variety of techniques and growth regulator combinations. From these studies, the growth regulator combination most successful for the uniform regeneration of variegated chimeras was 2.0 mg/l IAA and 2.0 mg/l kinetin. This combination promoted the least amount of callus formation and allowed for more uniform direct plantlet formation from the upper leaf surface. Adventitious shoot formation from callus showed more phenotypic differences in the variegated pattern and was therefore a less predictable method of propagation. Light microscopy was used to study the in vitro formation of adventitious shoots that developed directly from the adaxial leaf surface. The early event in these meristematic regions was xylem vessel formation in the upper spongy mesophyll cells located below two or more periclinally dividing epidermal cells. The majority of the meristematic activity appeared to be in these epidermal cells but subjacent palisade cells also showed cell divisions. The uniform regeneration of three different periclinal chimeras each showing a different LI, LII, LIII shoot apex organization, strongly suggested that the final formation of the shoot required a multicellular and multilayer development.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798661616531Subjects--Topical Terms:

516217
Botany.
Index Terms--Genre/Form:

542853
Electronic books.

A Histological and Ultrastructural Study of Variegated Leaf Chimeras of Saintpaulia Ionantha Wendl. and Their Regeneration in Vitro.
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100 1 $a Norris, Richard Eugene. $3 3700790
245 1 2 $a A Histological and Ultrastructural Study of Variegated Leaf Chimeras of Saintpaulia Ionantha Wendl. and Their Regeneration in Vitro.
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500 $a Source: Dissertations Abstracts International, Volume: 42-07, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
502 $a Thesis (Ph.D.)--Texas A&M University, 1981.
504 $a Includes bibliographical references
520 $a Variegated leaves of three African violet cultivars were studied by light and electron microscopy. This analysis combined with inheritance data revealed the following possible LI, LII, LIII periclinal chimera histogenic organizations: (a) cv. Marge Winters, GGW-type, (b) cv. Bold Dance, GWG-type, (c) cv. Calico Kitten, WWG-type. Electron micrographs of the mutated sections showed plastids that had dilated thylakoids typical of PSI mutants. Leaf and petiole explants of these variegated cultivars were cultured using a variety of techniques and growth regulator combinations. From these studies, the growth regulator combination most successful for the uniform regeneration of variegated chimeras was 2.0 mg/l IAA and 2.0 mg/l kinetin. This combination promoted the least amount of callus formation and allowed for more uniform direct plantlet formation from the upper leaf surface. Adventitious shoot formation from callus showed more phenotypic differences in the variegated pattern and was therefore a less predictable method of propagation. Light microscopy was used to study the in vitro formation of adventitious shoots that developed directly from the adaxial leaf surface. The early event in these meristematic regions was xylem vessel formation in the upper spongy mesophyll cells located below two or more periclinally dividing epidermal cells. The majority of the meristematic activity appeared to be in these epidermal cells but subjacent palisade cells also showed cell divisions. The uniform regeneration of three different periclinal chimeras each showing a different LI, LII, LIII shoot apex organization, strongly suggested that the final formation of the shoot required a multicellular and multilayer development.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Botany. $3 516217
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0479
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a Texas A&M University. $3 718977
773 0 $t Dissertations Abstracts International $g 42-07B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=8128916 $z click for full text (PQDT)