東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

Physiological Genomics of Temperature Adaption Among Natural Fundulus heteroclitus Populations.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Physiological Genomics of Temperature Adaption Among Natural Fundulus heteroclitus Populations./
作者:	DeLiberto, Amanda Nicole.
面頁冊數:	1 online resource (188 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
Contained By:	Dissertations Abstracts International84-12B.
標題:	Evolution & development. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30525133click for full text (PQDT)
ISBN:	9798379610708

Physiological Genomics of Temperature Adaption Among Natural Fundulus heteroclitus Populations.
DeLiberto, Amanda Nicole.

Physiological Genomics of Temperature Adaption Among Natural Fundulus heteroclitus Populations. - 1 online resource (188 pages)

Source: Dissertations Abstracts International, Volume: 84-12, Section: B.

Thesis (Ph.D.)--University of Miami, 2023.

Includes bibliographical references

Fluctuations in temperature govern an individual's physiology. Physiological responses to temperature may occur acutely, through acclimation, or through adaptation. Specifically, adaptation acting on physiological processes may occur if temperature differences are consistent, and drift is low. Yet characterizing temperature adaptation is challenging and an integrative approach combining physiological, molecular, and genomic data is necessary. Here six thermally distinct populations of the estuarine teleost, Fundulus heteroclitus are used to examine temperature adaptation. These populations are geographically close together, yet exhibit a non-clinal mosaic of habitat temperatures, allowing adaptive variation to be examined without the interference of demography. An integrative approach is applied by examining physiological variation, mRNA expression variation and genetic variation to understand temperature adaptation among these populations. First a framework for measuring cardiac metabolic rate was developed to increase throughput of the experiment. Next thermal tolerance (CTmax), standard metabolic rate (SMR), cardiac metabolic rate (CMR), and cardiac mRNA expression were measured at two acclimation temperatures and compared among populations. While there are some acclimation specific physiological responses, variation in physiological traits can also be explained by habitat temperature. Overall, there is up to 15-fold variation in an individual's response between acclimation temperatures (quantified here as thermal sensitivity - Q10). Similarly, there were significant differences in mRNA expression between acclimation temperatures. Differential mRNA expression within an acclimation temperature could also be explained by habitat temperature variation. This habitat and acclimation specific mRNA expression significantly explained variation in CTmax. Additionally, mRNA expression unrelated to habitat temperature explains significant variation in SMR, SMR Q10 and CMR. Finally, genetic variation among populations was examined to determine adaptive differences due to habitat between populations and characterize the genetic architecture of these physiological traits. A new method was developed to specifically sequenced the regulatory regions of expressed genes. While few direct temperature or trait associations were identified using a genomewide association analysis, 71 thermal-adaptive SNPs related to physiological traits were identified through the joint probability between each physiology and habitat temperature SNP. These thermal adaptive SNPs were related to physiological traits that were previously related to both habitat temperature and mRNA expression, revealing an adaptive role of these SNPs in physiological temperature adaptation among natural populations of F. heteroclitus. Together, these data confirm physiological differences among populations and suggest they are related to both acclimation and adaptive responses and adaptation.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798379610708Subjects--Topical Terms:

3172418
Evolution & development.
Subjects--Index Terms:

Gene expressionIndex Terms--Genre/Form:

542853
Electronic books.

Physiological Genomics of Temperature Adaption Among Natural Fundulus heteroclitus Populations.
LDR:04372nmm a2200397K 4500 001 2359460
005 20230917193956.5
006 m o d
007 cr mn ---uuuuu
008 241011s2023 xx obm 000 0 eng d
020 $a 9798379610708
035 $a (MiAaPQ)AAI30525133
035 $a AAI30525133
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a DeLiberto, Amanda Nicole. $3 3700063
245 1 0 $a Physiological Genomics of Temperature Adaption Among Natural Fundulus heteroclitus Populations.
264 0 $c 2023
300 $a 1 online resource (188 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
500 $a Advisor: Crawford, Douglas.
502 $a Thesis (Ph.D.)--University of Miami, 2023.
504 $a Includes bibliographical references
520 $a Fluctuations in temperature govern an individual's physiology. Physiological responses to temperature may occur acutely, through acclimation, or through adaptation. Specifically, adaptation acting on physiological processes may occur if temperature differences are consistent, and drift is low. Yet characterizing temperature adaptation is challenging and an integrative approach combining physiological, molecular, and genomic data is necessary. Here six thermally distinct populations of the estuarine teleost, Fundulus heteroclitus are used to examine temperature adaptation. These populations are geographically close together, yet exhibit a non-clinal mosaic of habitat temperatures, allowing adaptive variation to be examined without the interference of demography. An integrative approach is applied by examining physiological variation, mRNA expression variation and genetic variation to understand temperature adaptation among these populations. First a framework for measuring cardiac metabolic rate was developed to increase throughput of the experiment. Next thermal tolerance (CTmax), standard metabolic rate (SMR), cardiac metabolic rate (CMR), and cardiac mRNA expression were measured at two acclimation temperatures and compared among populations. While there are some acclimation specific physiological responses, variation in physiological traits can also be explained by habitat temperature. Overall, there is up to 15-fold variation in an individual's response between acclimation temperatures (quantified here as thermal sensitivity - Q10). Similarly, there were significant differences in mRNA expression between acclimation temperatures. Differential mRNA expression within an acclimation temperature could also be explained by habitat temperature variation. This habitat and acclimation specific mRNA expression significantly explained variation in CTmax. Additionally, mRNA expression unrelated to habitat temperature explains significant variation in SMR, SMR Q10 and CMR. Finally, genetic variation among populations was examined to determine adaptive differences due to habitat between populations and characterize the genetic architecture of these physiological traits. A new method was developed to specifically sequenced the regulatory regions of expressed genes. While few direct temperature or trait associations were identified using a genomewide association analysis, 71 thermal-adaptive SNPs related to physiological traits were identified through the joint probability between each physiology and habitat temperature SNP. These thermal adaptive SNPs were related to physiological traits that were previously related to both habitat temperature and mRNA expression, revealing an adaptive role of these SNPs in physiological temperature adaptation among natural populations of F. heteroclitus. Together, these data confirm physiological differences among populations and suggest they are related to both acclimation and adaptive responses and adaptation.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Evolution & development. $3 3172418
650 4 $a Genetics. $3 530508
650 4 $a Physiology. $3 518431
653 $a Gene expression
653 $a Metabolism
653 $a Thermal sensitivity
653 $a Thermal tolerance
653 $a Genomics
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0412
690 $a 0369
690 $a 0719
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a University of Miami. $b Marine Biology and Ecology (Marine). $3 3342591
773 0 $t Dissertations Abstracts International $g 84-12B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30525133 $z click for full text (PQDT)