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Genomic Analyses of Bacterial Blight Resistance, Fiber Quality Traits and Introgression of Thrips Resistance in Upland Cotton.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Genomic Analyses of Bacterial Blight Resistance, Fiber Quality Traits and Introgression of Thrips Resistance in Upland Cotton./
Author:	Shrestha, Navin.
Description:	1 online resource (256 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-01, Section: B.
Contained By:	Dissertations Abstracts International85-01B.
Subject:	Chromosomes. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30516393click for full text (PQDT)
ISBN:	9798379879433

Genomic Analyses of Bacterial Blight Resistance, Fiber Quality Traits and Introgression of Thrips Resistance in Upland Cotton.
Shrestha, Navin.

Genomic Analyses of Bacterial Blight Resistance, Fiber Quality Traits and Introgression of Thrips Resistance in Upland Cotton. - 1 online resource (256 pages)

Source: Dissertations Abstracts International, Volume: 85-01, Section: B.

Thesis (Ph.D.)--North Carolina State University, 2023.

Includes bibliographical references

Cotton (Gossypium spp.) is a globally important economic crop and a major source of natural fiber, oil, and protein. Upland cotton is prized for its lint but its production is impacted by the complicated relationship between fiber quality and yield. It is also threatened by various biotic factors such as cotton bacterial blight (CBB) caused by Xanthomonas citri subspecies malvacearum (Xcm) and insect pests like thrips (Franklineillaspp.).To study the genetic basis of fiber quality, yield and plant height traits, as well as resistance to Xcm and thrips, a series of genetic analyses were performed. To analyze the quantitative trait loci (QTL) responsible for fiber quality, yield and plant height traits, an F5:6 and F6:7 recombinant inbred line (RIL) population of 110 individuals, obtained through intraspecific G. hirsutumcrosses (NC05AZ06 x NC11-2100) was phenotyped for fiber quality, yield and plant height traits, and genotyped with Cotton 63K single nucleotide polymorphism (CottonSNP63K) array. We developed high-density linkage maps and identified 30 QTL on 15 different chromosomes, 14 of which were major QTL and three were major stable QTL. Candidate gene analysis of the major stable QTL of micronaire and fiber elongation and the plant height with the highest phenotypic variance explained (20.02% PVE) revealed five putative genes linked to fiber quality and yield traits and one to plant height trait.To identify the genomic region controlling resistance to Xcm, an F6 RIL population from a cross between Acala Maxaa x Arkot 8102 was developed. This population was genotyped with the CottonSNP63K array and phenotyped with Xcm race 18. The results of this analysis revealed that resistance to Xcm in Arkot 8102 was controlled by a single locus BB-13. The BB-13 locus was mapped within the 0.95 cM interval near the telomeric region in the long arm of chromosome D02. Flanking SNP markers, i04890Gh and i04907Gh of the BB-13 locus, identified from the linkage analysis, targeted it to a 371 Kb genomic region. Candidate gene analysis identified thirty putative gene sequences within this genomic region. Nine of these putative genes, and two NBS-LRR genes adjacent to the targeted region, were putatively involved in plant disease resistance and are possible candidate genes for BB-13locus.Interspecific hybridization and backcrossing was performed to introgress thrips resistance from Pima cotton (G. barbadense L.) cv. Coastland 320 (CL320) into Upland cotton (G. hirsutum L.) cvs. Acala Maxxa (AM) and Fiber Max 966 (FM966). Two backcross populations from interspecific crosses 'CL320 x AM' and 'CL320 x FM966' were developed and screened for thrips resistance in the field in summer 2020, 2021 and 2022. Eleven AM derived BC2F2 resistant introgression lines (ILs) and 21 FM966 derived BC2F2 resistant ILs were genotyped using CottonSNP63K array to identify the Pima introgressions. By using the introgression size values of ≥300 Kb for interstitial and ≥100 Kb for terminal introgressions, a total of 201 introgressions were identified in Acala Maxxa derived 11 BC2F2 ILs and 198 in Fiber Max 966 derived 21 BC2F2 ILs. Nine chromosomes, viz., A01, A08, A09, A10, A11, D10, D11, D12, and D13 were found to have contributed to thrips resistance. In conclusion, our current research findings; the identified QTL for fiber quality, yield and plant height traits, CBB resistant locus (BB13), introgression lines with thrips resistance, and the candidate genes could help breeders develop cotton cultivars with high fiber quality, increased yield, and improved resistance to biotic stresses.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798379879433Subjects--Topical Terms:

638948
Chromosomes.
Index Terms--Genre/Form:

542853
Electronic books.

Genomic Analyses of Bacterial Blight Resistance, Fiber Quality Traits and Introgression of Thrips Resistance in Upland Cotton.
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245 1 0 $a Genomic Analyses of Bacterial Blight Resistance, Fiber Quality Traits and Introgression of Thrips Resistance in Upland Cotton.
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500 $a Source: Dissertations Abstracts International, Volume: 85-01, Section: B.
500 $a Advisor: Skaar, David; Kuraparthy, Vasu.
502 $a Thesis (Ph.D.)--North Carolina State University, 2023.
504 $a Includes bibliographical references
520 $a Cotton (Gossypium spp.) is a globally important economic crop and a major source of natural fiber, oil, and protein. Upland cotton is prized for its lint but its production is impacted by the complicated relationship between fiber quality and yield. It is also threatened by various biotic factors such as cotton bacterial blight (CBB) caused by Xanthomonas citri subspecies malvacearum (Xcm) and insect pests like thrips (Franklineillaspp.).To study the genetic basis of fiber quality, yield and plant height traits, as well as resistance to Xcm and thrips, a series of genetic analyses were performed. To analyze the quantitative trait loci (QTL) responsible for fiber quality, yield and plant height traits, an F5:6 and F6:7 recombinant inbred line (RIL) population of 110 individuals, obtained through intraspecific G. hirsutumcrosses (NC05AZ06 x NC11-2100) was phenotyped for fiber quality, yield and plant height traits, and genotyped with Cotton 63K single nucleotide polymorphism (CottonSNP63K) array. We developed high-density linkage maps and identified 30 QTL on 15 different chromosomes, 14 of which were major QTL and three were major stable QTL. Candidate gene analysis of the major stable QTL of micronaire and fiber elongation and the plant height with the highest phenotypic variance explained (20.02% PVE) revealed five putative genes linked to fiber quality and yield traits and one to plant height trait.To identify the genomic region controlling resistance to Xcm, an F6 RIL population from a cross between Acala Maxaa x Arkot 8102 was developed. This population was genotyped with the CottonSNP63K array and phenotyped with Xcm race 18. The results of this analysis revealed that resistance to Xcm in Arkot 8102 was controlled by a single locus BB-13. The BB-13 locus was mapped within the 0.95 cM interval near the telomeric region in the long arm of chromosome D02. Flanking SNP markers, i04890Gh and i04907Gh of the BB-13 locus, identified from the linkage analysis, targeted it to a 371 Kb genomic region. Candidate gene analysis identified thirty putative gene sequences within this genomic region. Nine of these putative genes, and two NBS-LRR genes adjacent to the targeted region, were putatively involved in plant disease resistance and are possible candidate genes for BB-13locus.Interspecific hybridization and backcrossing was performed to introgress thrips resistance from Pima cotton (G. barbadense L.) cv. Coastland 320 (CL320) into Upland cotton (G. hirsutum L.) cvs. Acala Maxxa (AM) and Fiber Max 966 (FM966). Two backcross populations from interspecific crosses 'CL320 x AM' and 'CL320 x FM966' were developed and screened for thrips resistance in the field in summer 2020, 2021 and 2022. Eleven AM derived BC2F2 resistant introgression lines (ILs) and 21 FM966 derived BC2F2 resistant ILs were genotyped using CottonSNP63K array to identify the Pima introgressions. By using the introgression size values of ≥300 Kb for interstitial and ≥100 Kb for terminal introgressions, a total of 201 introgressions were identified in Acala Maxxa derived 11 BC2F2 ILs and 198 in Fiber Max 966 derived 21 BC2F2 ILs. Nine chromosomes, viz., A01, A08, A09, A10, A11, D10, D11, D12, and D13 were found to have contributed to thrips resistance. In conclusion, our current research findings; the identified QTL for fiber quality, yield and plant height traits, CBB resistant locus (BB13), introgression lines with thrips resistance, and the candidate genes could help breeders develop cotton cultivars with high fiber quality, increased yield, and improved resistance to biotic stresses.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Chromosomes. $3 638948
650 4 $a Nitrates. $3 914879
650 4 $a Cotton. $3 865960
650 4 $a Seeds. $3 573605
650 4 $a Agronomy. $3 2122783
650 4 $a Genetics. $3 530508
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0285
690 $a 0369
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a North Carolina State University. $3 1018772
773 0 $t Dissertations Abstracts International $g 85-01B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30516393 $z click for full text (PQDT)