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Should I Stay or Should I Go? Environmental Factors Shape V. fischeri-Squid Distribution and Mutualism Dynamics.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Should I Stay or Should I Go? Environmental Factors Shape V. fischeri-Squid Distribution and Mutualism Dynamics./
Author:	Coryell, Randy Lane.
Description:	1 online resource (123 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
Contained By:	Dissertations Abstracts International84-12B.
Subject:	Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30419906click for full text (PQDT)
ISBN:	9798379733902

Should I Stay or Should I Go? Environmental Factors Shape V. fischeri-Squid Distribution and Mutualism Dynamics.
Coryell, Randy Lane.

Should I Stay or Should I Go? Environmental Factors Shape V. fischeri-Squid Distribution and Mutualism Dynamics. - 1 online resource (123 pages)

Source: Dissertations Abstracts International, Volume: 84-12, Section: B.

Thesis (Ph.D.)--New Mexico State University, 2023.

Includes bibliographical references

Natural and anthropogenic environmental change can impact biodiversity by causing chemical disequilibria and shrinking habitats around the globe. Here I ask what impact some of these changes will have on animal-microbe interactions in the ocean. I test three hypotheses in an attempt to answer this question using a model binary symbiosis, the squid-bioluminescent bacterium (Euprymna-Vibrio) mutualism. In the Philippines, I tested if biogeographical barriers were similarly impactful on host and microbes. I expected that there would be a significant impact of geography on host and microbe population structure. Fourteen separate sites in the Philippine archipelago were examined using haplotype estimates that were examined via nested clade analysis to determine the relationship between Euprymna albatrossae and Vibrio fischeri populations and their geographic locations. Host animal population structure showed significant effects while symbionts were distributed randomly. Seasonal currents and deep-water thermoclines are apparently biogeographic phenomena impacting the biological community structures of this system.In another study, I exposed five different strains of V. fischeri to multiple species of Euprymna (E. scolopes, E. morsei, E. tasmanica, and E. albatrossae) that were wild-caught from across the IndoWest Pacific. Multi-generational adaptation at five temperatures for each of the bacterial strains was performed in vitro, testing if temperatures within and without the bacteria's normal temperature range impacted colonization, physiology, and bacterial competitive ability. I compared endpoint and midpoint adapted lines to their common ancestor using growth rate, bioluminescence, motility, and biofilm formation as measures of colonization physiology. Changes in luminescence were observed particularly in lines adapted to elevated temperatures relative to native range. Colonization physiology was found to be impacted by adaptation to novel temperature in vitro lending support to our second hypothesis that strains of V. fischeri would respond differently depending on geographic range and host animal. A third hypothesis was tested, that colonization efficiency will be impacted with these temperature-adapted strains of V. fischeri when encountering juvenile E. scolopes. Adaptation to novel temperature did impact colonization efficiency, and in some cases, adaptation at the stressful temperatures increased strain competitiveness when colonizing juvenile squid. Adaptation to high temperature stress, (i.e., 32℃) negatively affected different physiological traits, yet four of the five high temperature adapted strains outcompeted the ancestor at the neutral colonization temperature of 28℃. Overall, our results demonstrate that microbes and their hosts will be challenged by global temperature change. Biodiversity would also be impacted if these mutualist partners were to experience undesirable conditions like those tested here, pointing to a need to further investigate the dynamic of environmental stress on animal microbe interactions.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798379733902Subjects--Topical Terms:

536250
Microbiology.
Subjects--Index Terms:

CoevolutionIndex Terms--Genre/Form:

542853
Electronic books.

Should I Stay or Should I Go? Environmental Factors Shape V. fischeri-Squid Distribution and Mutualism Dynamics.
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500 $a Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
500 $a Advisor: Smith, Geoffrey.
502 $a Thesis (Ph.D.)--New Mexico State University, 2023.
504 $a Includes bibliographical references
520 $a Natural and anthropogenic environmental change can impact biodiversity by causing chemical disequilibria and shrinking habitats around the globe. Here I ask what impact some of these changes will have on animal-microbe interactions in the ocean. I test three hypotheses in an attempt to answer this question using a model binary symbiosis, the squid-bioluminescent bacterium (Euprymna-Vibrio) mutualism. In the Philippines, I tested if biogeographical barriers were similarly impactful on host and microbes. I expected that there would be a significant impact of geography on host and microbe population structure. Fourteen separate sites in the Philippine archipelago were examined using haplotype estimates that were examined via nested clade analysis to determine the relationship between Euprymna albatrossae and Vibrio fischeri populations and their geographic locations. Host animal population structure showed significant effects while symbionts were distributed randomly. Seasonal currents and deep-water thermoclines are apparently biogeographic phenomena impacting the biological community structures of this system.In another study, I exposed five different strains of V. fischeri to multiple species of Euprymna (E. scolopes, E. morsei, E. tasmanica, and E. albatrossae) that were wild-caught from across the IndoWest Pacific. Multi-generational adaptation at five temperatures for each of the bacterial strains was performed in vitro, testing if temperatures within and without the bacteria's normal temperature range impacted colonization, physiology, and bacterial competitive ability. I compared endpoint and midpoint adapted lines to their common ancestor using growth rate, bioluminescence, motility, and biofilm formation as measures of colonization physiology. Changes in luminescence were observed particularly in lines adapted to elevated temperatures relative to native range. Colonization physiology was found to be impacted by adaptation to novel temperature in vitro lending support to our second hypothesis that strains of V. fischeri would respond differently depending on geographic range and host animal. A third hypothesis was tested, that colonization efficiency will be impacted with these temperature-adapted strains of V. fischeri when encountering juvenile E. scolopes. Adaptation to novel temperature did impact colonization efficiency, and in some cases, adaptation at the stressful temperatures increased strain competitiveness when colonizing juvenile squid. Adaptation to high temperature stress, (i.e., 32℃) negatively affected different physiological traits, yet four of the five high temperature adapted strains outcompeted the ancestor at the neutral colonization temperature of 28℃. Overall, our results demonstrate that microbes and their hosts will be challenged by global temperature change. Biodiversity would also be impacted if these mutualist partners were to experience undesirable conditions like those tested here, pointing to a need to further investigate the dynamic of environmental stress on animal microbe interactions.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Microbiology. $3 536250
650 4 $a Ecology. $3 516476
650 4 $a Evolution & development. $3 3172418
653 $a Coevolution
653 $a Mutualism
653 $a Phylogeography
653 $a Colonization physiology
653 $a Animal population
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710 2 $a New Mexico State University. $b Biology. $3 3700008
773 0 $t Dissertations Abstracts International $g 84-12B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30419906 $z click for full text (PQDT)