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Endosomes and mitosis : = FIP3-associated vesicle delivery during cytokinesis.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Endosomes and mitosis :/
其他題名:	FIP3-associated vesicle delivery during cytokinesis.
作者:	Simon, Glenn C.
面頁冊數:	1 online resource (116 pages)
附註:	Source: Dissertations Abstracts International, Volume: 70-11, Section: B.
Contained By:	Dissertations Abstracts International70-11B.
標題:	Molecular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3343396click for full text (PQDT)
ISBN:	9780549982012

Endosomes and mitosis : = FIP3-associated vesicle delivery during cytokinesis.
Simon, Glenn C.

Endosomes and mitosis :FIP3-associated vesicle delivery during cytokinesis. - 1 online resource (116 pages)

Source: Dissertations Abstracts International, Volume: 70-11, Section: B.

Thesis (Ph.D.)--University of Colorado Health Sciences Center, 2008.

Includes bibliographical references

Cytokinesis is a highly regulated event that involves many different cellular pathways. Two distinct processes play an important role during cytokinesis: remodeling of cellular cytoskeleton and the delivery of new membranes to the cleavage furrow. Thus, tightly controlled temporal and spatial coordination of cytoskeleton remodeling and membrane traffic is required for the successful completion of cell division. Indeed, the delivery of endocytic membranes to the cleavage furrow has been shown to play a key role in furrowing and abscission. Recently we have shown that FIP3, a Rab11 GTPase binding protein, mediates the targeting of recycling endosomes to the midbody of dividing cells and is required for cytokinesis. We also have demonstrated that FIP3 interacts with the centralspindlin protein complex and that this interaction is required for the targeting of the endosomes to the midbody of mitotic cells. In this study we investigate the molecular machinery regulating FIP3 targeting to the cleavage furrow. First, we show that FIP3 and ECT2 (a RhoA GEF and centralspindlin-binding protein) compete for the binding to the centralspindlin protein complex and that the removal of ECT2 from the centralspindlin complex is required for FIP3 targeting to the midbody and completion of cytokinesis. Second, we show that calcium is also required for the recruitment of FIP3 to the midbody. We also demonstrate that calcium regulates FIP3 targeting to the midbody through its binding to the putative EF hands in FIP3. Lastly, we investigate the components and constituents of FIP3 associated vesicles in order to better understand why their delivery to the midbody is important. In summary, we propose that the centralspindlin complex plays a role in temporal and spatial regulation of actomyosin ring contraction and endocytic vesicle transport to the midbody through sequential interactions with ECT2 and FIP3. Furthermore, the delivery of FIP3 to the midbody is calcium dependent. These regulated and dynamic events are critical for the delivery of many proteins identified as cargo of FIP3-associated vesicles, and also for the final abscission of the daughter cells.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9780549982012Subjects--Topical Terms:

517296
Molecular biology.
Subjects--Index Terms:

CentralspindlinIndex Terms--Genre/Form:

542853
Electronic books.

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