東華大學圖書館 |

Natural and Synthetic Silica Incorporated Chitosan Composite Scaffolds for Bone Tissue Engineering Applications = = Kemik Doku Muhendisligi Uygulamalarina Yonelik Dogal ve Sentetik Silika Katkili Kitosan Kompozit Doku Iskeleleri.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Natural and Synthetic Silica Incorporated Chitosan Composite Scaffolds for Bone Tissue Engineering Applications =/
其他題名:	Kemik Doku Muhendisligi Uygulamalarina Yonelik Dogal ve Sentetik Silika Katkili Kitosan Kompozit Doku Iskeleleri.
其他題名:	Kemik Doku Muhendisligi Uygulamalarina Yonelik Dogal ve Sentetik Silika Katkili Kitosan Kompozit Doku Iskeleleri.
作者:	Tamburaci, Sedef.
面頁冊數:	1 online resource (176 pages)
附註:	Source: Dissertations Abstracts International, Volume: 83-11, Section: B.
Contained By:	Dissertations Abstracts International83-11B.
標題:	Tissue engineering. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29079117click for full text (PQDT)
ISBN:	9798426881532

Natural and Synthetic Silica Incorporated Chitosan Composite Scaffolds for Bone Tissue Engineering Applications = = Kemik Doku Muhendisligi Uygulamalarina Yonelik Dogal ve Sentetik Silika Katkili Kitosan Kompozit Doku Iskeleleri.
Tamburaci, Sedef.

Natural and Synthetic Silica Incorporated Chitosan Composite Scaffolds for Bone Tissue Engineering Applications =Kemik Doku Muhendisligi Uygulamalarina Yonelik Dogal ve Sentetik Silika Katkili Kitosan Kompozit Doku Iskeleleri.Kemik Doku Muhendisligi Uygulamalarina Yonelik Dogal ve Sentetik Silika Katkili Kitosan Kompozit Doku Iskeleleri. - 1 online resource (176 pages)

Source: Dissertations Abstracts International, Volume: 83-11, Section: B.

Thesis (Ph.D.)--Izmir Institute of Technology (Turkey), 2016.

Includes bibliographical references

Recently bone tissue engineering studies have focused on the development of 3D scaffolds that can organize the tissue regeneration in natural way with appropriate porosity and reinforced the structure. Natural polymer-based composites have been focused with more attention than synthetic polymer composites for bone tissue engineering applications because of their biocompatibility and biodegradability. In this work, the goal was to combine the useful biomaterial properties of both chitosan and silica to design biocomposite organic/inorganic biomaterials for bone tissue engineering applications. The composite scaffolds were fabricated by freeze drying method bu using two different silicas; natural silica; Diatomite and synthetic silica, octa (tetramethylammonium) polyhedral oligomeric silsesquioxanes (OctaTMA-POSS).The effects of silica type and loading on the mechanical, morphological, chemical, surface properties, wettability and biocompatibility of composite scaffolds were investigated and characterized by using SEM, AFM, contact angle analysis, swelling study, protein adsorption assay, biodegradation and biomineralization tests. WST-I cytotoxicity, cell proliferation with rezasurin and alkaline phosphatase activity assays were performed to determine biological activity of the composite scaffolds. In vitro biomineralization on scaffolds was determined by Von Kossa and Alizarin red staining. POSS and diatomite incorporation increased the surface roughness. Chitosan- silica composites exhibited 82-90% porosity. Wet chitosan-silica composite scaffolds exhibited higher compression moduli compared to pure chitosan scaffold in 67.3- 81.4kPa and 78.1 to 107.6kPa range respectively. Average pore size range of chitosan- diatomite and chitosan-POSS composite scaffolds was obtained as 15-180µm and 220- 300um, respectively. Results indicated that chitosan-silica composites did not show any cytotoxic effect on 3T3, MG-63 and Saos-2 cell lines. Chitosan-silica composites were found to be favorable for osteoblast proliferation. Diatomite and POSS incorporation showed promising effects with enhancing ALP activity on hFob cells. Therefore, these composite scaffolds could be used for bone tissue engineering applications.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798426881532Subjects--Topical Terms:

823582
Tissue engineering.
Index Terms--Genre/Form:

542853
Electronic books.

Natural and Synthetic Silica Incorporated Chitosan Composite Scaffolds for Bone Tissue Engineering Applications = = Kemik Doku Muhendisligi Uygulamalarina Yonelik Dogal ve Sentetik Silika Katkili Kitosan Kompozit Doku Iskeleleri.
LDR:05961nmm a2200445K 4500 001 2357898
005 20230725053807.5
006 m o d
007 cr mn ---uuuuu
008 241011s2016 xx obm 000 0 eng d
020 $a 9798426881532
035 $a (MiAaPQ)AAI29079117
035 $a (MiAaPQ)IzmirTech4851
035 $a AAI29079117
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Tamburaci, Sedef. $3 3698425
245 1 0 $a Natural and Synthetic Silica Incorporated Chitosan Composite Scaffolds for Bone Tissue Engineering Applications = $b Kemik Doku Muhendisligi Uygulamalarina Yonelik Dogal ve Sentetik Silika Katkili Kitosan Kompozit Doku Iskeleleri.
246 3 1 $a Kemik Doku Muhendisligi Uygulamalarina Yonelik Dogal ve Sentetik Silika Katkili Kitosan Kompozit Doku Iskeleleri.
264 0 $c 2016
300 $a 1 online resource (176 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 83-11, Section: B.
500 $a Advisor: Tihminlioglu, Funda; Havitcioglu, Hasan.
502 $a Thesis (Ph.D.)--Izmir Institute of Technology (Turkey), 2016.
504 $a Includes bibliographical references
520 $a Recently bone tissue engineering studies have focused on the development of 3D scaffolds that can organize the tissue regeneration in natural way with appropriate porosity and reinforced the structure. Natural polymer-based composites have been focused with more attention than synthetic polymer composites for bone tissue engineering applications because of their biocompatibility and biodegradability. In this work, the goal was to combine the useful biomaterial properties of both chitosan and silica to design biocomposite organic/inorganic biomaterials for bone tissue engineering applications. The composite scaffolds were fabricated by freeze drying method bu using two different silicas; natural silica; Diatomite and synthetic silica, octa (tetramethylammonium) polyhedral oligomeric silsesquioxanes (OctaTMA-POSS).The effects of silica type and loading on the mechanical, morphological, chemical, surface properties, wettability and biocompatibility of composite scaffolds were investigated and characterized by using SEM, AFM, contact angle analysis, swelling study, protein adsorption assay, biodegradation and biomineralization tests. WST-I cytotoxicity, cell proliferation with rezasurin and alkaline phosphatase activity assays were performed to determine biological activity of the composite scaffolds. In vitro biomineralization on scaffolds was determined by Von Kossa and Alizarin red staining. POSS and diatomite incorporation increased the surface roughness. Chitosan- silica composites exhibited 82-90% porosity. Wet chitosan-silica composite scaffolds exhibited higher compression moduli compared to pure chitosan scaffold in 67.3- 81.4kPa and 78.1 to 107.6kPa range respectively. Average pore size range of chitosan- diatomite and chitosan-POSS composite scaffolds was obtained as 15-180µm and 220- 300um, respectively. Results indicated that chitosan-silica composites did not show any cytotoxic effect on 3T3, MG-63 and Saos-2 cell lines. Chitosan-silica composites were found to be favorable for osteoblast proliferation. Diatomite and POSS incorporation showed promising effects with enhancing ALP activity on hFob cells. Therefore, these composite scaffolds could be used for bone tissue engineering applications.
520 $a Son donemde kemik doku muhendisligi calismalari uygun poroziteye ve guclendirilmis yapiya sahip, doku rejenerasyonunu organize eden uc boyutlu yapi iskeleleri uzerinde yogunlasmistir. Kemik doku muhendisligi uygulamalarinda dogal polimer bazli kompozitler biyouyumlu ve biyobozunur ozellikleri yuzunden sentetik kompozitlere gore daha cok ilgi cekmektedir. Bu calismada amac, kitosan ve silikanin faydali biyomalzeme ozelliklerini biaraya getirerek kemik doku muhendisligi uygulamalarina yonelik inorganik/organik biyokompozit biyomalzeme tasarlamaktir. Kompozit yapi iskeleleri iki farkli silika kaynagi; dogal silika, diatom ve sentetik silika okta-tetrametilamonyum polihedral oligomerik silseskioksan (OctaTMA-POSS) kullanilarak dondurarak kurutma yontemi ile uretilmistir. Silika turu ve katkisinin, kompozitlerin mekanik, morfolojik, kimyasal ve yuzey ozellikleri, islanirlik ve biyouyumlulugu uzerine etkisi incelenmis ve temas acisi analizi, su absorpsiyon calismalari, biyobozunurluk ve biyomineralizasyon testleri ile karakterize edilmistir. Kompozit yapi iskelelerinin biyolojik aktivitesi WST-1 sitotoksisite testi, rezasurin ile hucre proliferasyonu ve alkalen fosfataz aktivite testi ile belirlenmistir. Kompozitler uzerindeki in vitro biyomineralizasyon von Kossa ve Alizarin red boyama yapilarak belirlenmistir. Kitosan-silika kompozitler 82-90% gozeneklilik orani sergilemistir. Islak kitosan-diatom ve kitosan-POSS kompozit yapi iskelelerinde saf kitosana kiyasla, 67.3- 81.4kPa ve 78.1-107.6kPa araliginda daha yuksek basma modulusu elde edilmistir. Kitosan-diatom ve kitosan-POSS kompozitlerin ortalama gozenek capi sirayla 15- 180µm ve 220-300µm olarak olculmustur. POSS ve diatom katkilari yuzet puruzlulugunu arttirmistir. Kitosan-silika kompozitler 3T3, MG-63 ve Saos-2 hucreleri uzerinde sitotoksik etki gostermemistir, hucre proliferasyonu icin uygun bulunmustur. Diatom and POSS katkilari, hFob hucrelerinin ALP aktivitesini arttirarak gelecek vaad eden bir etki gostermistir.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Tissue engineering. $3 823582
650 4 $a Cancer. $3 634186
650 4 $a Materials research. $3 3686999
650 4 $a Internships. $3 3560137
650 4 $a Wound healing. $3 825377
650 4 $a Biochemistry. $3 518028
650 4 $a Carbohydrates. $3 522687
650 4 $a Bones. $3 634311
650 4 $a Adsorption. $3 580046
650 4 $a Chemistry. $3 516420
650 4 $a Biomedical engineering. $3 535387
650 4 $a Skin. $3 843661
650 4 $a Biomedical materials. $3 605745
650 4 $a Orthopedics. $3 705991
650 4 $a Composite materials. $3 654082
650 4 $a Polymers. $3 535398
650 4 $a Medicine. $3 641104
650 4 $a Materials science. $3 543314
650 4 $a Bioengineering. $3 657580
650 4 $a Presentations. $3 3684537
650 4 $a Biotechnology. $3 571461
650 4 $a Interfaces. $2 gtt $3 834756
650 4 $a Engineering. $3 586835
650 4 $a Polymer chemistry. $3 3173488
650 4 $a Surgery. $3 707153
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0202
690 $a 0541
690 $a 0564
690 $a 0487
690 $a 0485
690 $a 0794
690 $a 0537
690 $a 0495
690 $a 0576
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a Izmir Institute of Technology (Turkey). $3 3561138
773 0 $t Dissertations Abstracts International $g 83-11B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29079117 $z click for full text (PQDT)