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Biotransformation of Biomass Waste Residues into Value-Added Chemicals Using Filamentous Fungi.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Biotransformation of Biomass Waste Residues into Value-Added Chemicals Using Filamentous Fungi./
作者:	Troiano, Derek.
面頁冊數:	1 online resource (209 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-10, Section: B.
Contained By:	Dissertations Abstracts International84-10B.
標題:	Catalytic oxidation. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30347019click for full text (PQDT)
ISBN:	9798377670261

Biotransformation of Biomass Waste Residues into Value-Added Chemicals Using Filamentous Fungi.
Troiano, Derek.

Biotransformation of Biomass Waste Residues into Value-Added Chemicals Using Filamentous Fungi. - 1 online resource (209 pages)

Source: Dissertations Abstracts International, Volume: 84-10, Section: B.

Thesis (Ph.D.)--McGill University (Canada), 2022.

Includes bibliographical references

Use of biomass as feedstock for the production of carbon-based fuels and chemicals represents an essential component of the global strategy to attain sustainable and circular industrial economies. Current strategic objectives for biomass conversion technologies focus on enhancing cost competitiveness through, for example, the use of cheaper feedstocks (e.g. lignocellulosic wastes). However, adoption of lignocellulosic biomass as substrate will only be possible after addressing the inefficiencies and costs which stem from the additional processing necessitated by the compositional complexity of lignocellulosic materials. Nature, wherein the recycling of lignocellulosics is an essential ecological process, may offer strategies for engineers and scientists to emulate.Fungi, particularly filamentous fungi, are well-known for their role as material recyclers in the natural carbon cycles and, as such, are intriguing for use in biomass-conversion, or biorefining processes. Concerning the products of fungal biotransformations, the number of potential products is proportional to the number of metabolites associated with a given organism; and these metabolic products remain largely underexplored or altogether unidentified. Therefore, a method for screening and evaluating a large number of products that may be obtained from fungal biotransformation of various biomass feedstocks was developed as part of the study described herein. Identified among the products were valuable chemicals, such as those described in two recent US Department of Energy surveys, including furans and organic acids. In addition to these "top chemical opportunities", substantial production of bio-pigments was also observed. Of the feedstocks investigated, a simulated food-waste feedstock was associated with the highest pigment production and was thus further investigated for maximal pigment production. To that end, an optimized extraction method was employed and, following this, a novel strategy was explored involving co-culturing of multiple strains of fungi together in a single conversion reaction. It was found that co-culturing the pigment-producing fungus with a specific second species elicited significantly more pigment production vis-a-vis the pigment-producing species alone.2,5-furandicarboxylic acid (FDCA), a compound of industrial interest for use as a replacement for terephthalic acid in polymer production, was also identified among the fungal biotransformation products of apple pomace feedstock. Since FDCA is mainly produced via oxidation of 5-hydroxymethylfurfural (HMF), itself a carbohydrate-derived product, the process of using filamentous fungi for converting HMF into FDCA was more closely investigated. Several industrially relevant strains of filamentous fungi, including the strain used throughout this project (Talaromyces sp. NRRL 2120), were screened for oxidative activity on HMF. It was found that multiple strains of fungi readily performed one oxidation on HMF (i.e. converted HMF into its acid counterpart) but accumulated very little, if any, FDCA under the given reaction conditions. To enable additional oxidation steps, a chemo-biocatalytic cascade involving the best-performing fungal strain coupled with an enzyme/mediator system was devised. This system enabled full conversion of HMF into FDCA and achieved the highest yield and productivity for any system involving filamentous-fungal whole-cells. Following this, the ability to reuse the whole-cell biocatalysts for multiple reactions was explored via encapsulation of the filamentous fungal whole cells. Here it was found that encapsulation of the fungal cells in Ca-alginate beads enabled the biocatalysts to be reliably recycled for up to nine reaction cycles.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798377670261Subjects--Topical Terms:

3560642
Catalytic oxidation.
Index Terms--Genre/Form:

542853
Electronic books.

Biotransformation of Biomass Waste Residues into Value-Added Chemicals Using Filamentous Fungi.
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500 $a Source: Dissertations Abstracts International, Volume: 84-10, Section: B.
500 $a Advisor: Orsat, Valerie ; Dumont, Marie-Josee.
502 $a Thesis (Ph.D.)--McGill University (Canada), 2022.
504 $a Includes bibliographical references
520 $a Use of biomass as feedstock for the production of carbon-based fuels and chemicals represents an essential component of the global strategy to attain sustainable and circular industrial economies. Current strategic objectives for biomass conversion technologies focus on enhancing cost competitiveness through, for example, the use of cheaper feedstocks (e.g. lignocellulosic wastes). However, adoption of lignocellulosic biomass as substrate will only be possible after addressing the inefficiencies and costs which stem from the additional processing necessitated by the compositional complexity of lignocellulosic materials. Nature, wherein the recycling of lignocellulosics is an essential ecological process, may offer strategies for engineers and scientists to emulate.Fungi, particularly filamentous fungi, are well-known for their role as material recyclers in the natural carbon cycles and, as such, are intriguing for use in biomass-conversion, or biorefining processes. Concerning the products of fungal biotransformations, the number of potential products is proportional to the number of metabolites associated with a given organism; and these metabolic products remain largely underexplored or altogether unidentified. Therefore, a method for screening and evaluating a large number of products that may be obtained from fungal biotransformation of various biomass feedstocks was developed as part of the study described herein. Identified among the products were valuable chemicals, such as those described in two recent US Department of Energy surveys, including furans and organic acids. In addition to these "top chemical opportunities", substantial production of bio-pigments was also observed. Of the feedstocks investigated, a simulated food-waste feedstock was associated with the highest pigment production and was thus further investigated for maximal pigment production. To that end, an optimized extraction method was employed and, following this, a novel strategy was explored involving co-culturing of multiple strains of fungi together in a single conversion reaction. It was found that co-culturing the pigment-producing fungus with a specific second species elicited significantly more pigment production vis-a-vis the pigment-producing species alone.2,5-furandicarboxylic acid (FDCA), a compound of industrial interest for use as a replacement for terephthalic acid in polymer production, was also identified among the fungal biotransformation products of apple pomace feedstock. Since FDCA is mainly produced via oxidation of 5-hydroxymethylfurfural (HMF), itself a carbohydrate-derived product, the process of using filamentous fungi for converting HMF into FDCA was more closely investigated. Several industrially relevant strains of filamentous fungi, including the strain used throughout this project (Talaromyces sp. NRRL 2120), were screened for oxidative activity on HMF. It was found that multiple strains of fungi readily performed one oxidation on HMF (i.e. converted HMF into its acid counterpart) but accumulated very little, if any, FDCA under the given reaction conditions. To enable additional oxidation steps, a chemo-biocatalytic cascade involving the best-performing fungal strain coupled with an enzyme/mediator system was devised. This system enabled full conversion of HMF into FDCA and achieved the highest yield and productivity for any system involving filamentous-fungal whole-cells. Following this, the ability to reuse the whole-cell biocatalysts for multiple reactions was explored via encapsulation of the filamentous fungal whole cells. Here it was found that encapsulation of the fungal cells in Ca-alginate beads enabled the biocatalysts to be reliably recycled for up to nine reaction cycles.
520 $a L'utilisation de la biomasse comme matiere premiere pour la production de carburants et de produits chimiques represente un element essentiel de la strategie mondiale visant a mettre en place des economies industrielles durables et circulaires. Les objectifs actuels a la realisation des technologies de conversion de la biomasse sont axes sur l'amelioration de la competitivite des couts, notamment par l'utilisation de matieres premieres moins couteuses (par exemple, les dechets lignocellulosiques). Cependant, l'utilisation de produits lignocellulosiques ne sera possible qu'apres avoir resolu les inefficacites et les couts dus aux etapes additionnelles de transformation requises en raison de la complexite compositionnelle des matieres lignocellulosiques. La nature, ou le recyclage des matieres lignocellulosiques est un processus ecologique essentiel, peut offrir des modeles de strategies que les ingenieurs et les scientifiques pourront imiter.Les mycetes, en particulier les mycetes filamenteux, sont bien connus pour leur role de recycleurs de materiaux dans les cycles naturels du carbone et, a ce titre, ils sont d'interessants candidats a prendre en consideration dans les processus de conversion de la biomasse (bioraffinage). L'application des mycetes filamenteux dans les bioraffineries a donc ete examinee dans le cadre d'une revue exhaustive de la litterature (Chapitre 2). Cette revue litteraire permet de conclure que, si aucune souche de mycete, voire aucun micro-organisme, ne peut realiser a lui seul toutes les etapes de la conversion de la biomasse (du pretraitement de la biomasse brute a l'accumulation des produits souhaites), la valorisation complete de la biomasse est possible en employant et/ou en combinant plusieurs souches fongiques a des etapes specifiques du processus et en ciblant plusieurs produits. En ce qui concerne les produits des biotransformations fongiques, le nombre de produits potentiels est proportionnel au nombre de metabolites associes a un organisme donne, et ces produits metaboliques restent largement sousexplores ou totalement non identifies. Par consequent, une methode de criblage et d'evaluation du grand nombre de produits pouvant etre obtenus par la biotransformation fongique de matieres premieres de la biomasse a ete mise au point dans le cadre de l'etude decrite au chapitre 3. Les produits issus des biotransformations ont ete identifies a l'aide d'une chromatographie liquide ultra-performante non ciblee couplee a une spectrometrie de masse par electronebulisation a temps de vol quadripolaire et evalues a l'aide d'outils statistiques multivaries couramment utilises dans le domaine de la metabolomique. Parmi les produits identifies, on retrouve des produits chimiques extremement interessants tels que ceux decrits dans deux etudes recentes du ministere americains de l'energie, notamment les furanes et les acides organiques. En plus de ces composes, une production substantielle de biopigments a egalement ete observee. Parmi les matieres premieres etudiees, la simulation de la fermentation des dechets alimentaires a ete associee a la production de pigments la plus elevee et a donc fait l'objet d'une etude plus approfondie en vue d'une production accrue de pigments (chapitre 4). A cette fin, une nouvelle methode d'extraction optimisee a d'abord ete employee, puis une nouvelle strategie a ete exploree, impliquant la co-culture de plusieurs souches de champignons dans une seule reaction de conversion.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
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650 4 $a Fermentation. $3 658044
650 4 $a Fungi. $3 571472
650 4 $a Polylactic acid. $3 3297102
650 4 $a Biodiesel fuels. $3 587935
650 4 $a Pigments. $3 599727
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650 4 $a Cellulose. $3 588995
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650 4 $a Alternative energy sources. $3 3561089
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650 4 $a Enzymes. $3 520899
650 4 $a Biotechnology. $3 571461
650 4 $a Metabolites. $3 683644
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650 4 $a Alternative energy. $3 3436775
650 4 $a Chemical engineering. $3 560457
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