東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

Characterization of Bacterial Isolates from Carpenter Bees for Biodegradation of Lignocellulose Compounds.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Characterization of Bacterial Isolates from Carpenter Bees for Biodegradation of Lignocellulose Compounds./
作者:	Cuebas-Irizarry, Mara Francheska.
面頁冊數:	1 online resource (209 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-09, Section: B.
Contained By:	Dissertations Abstracts International84-09B.
標題:	Fungi. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30283627click for full text (PQDT)
ISBN:	9798374431834

Characterization of Bacterial Isolates from Carpenter Bees for Biodegradation of Lignocellulose Compounds.
Cuebas-Irizarry, Mara Francheska.

Characterization of Bacterial Isolates from Carpenter Bees for Biodegradation of Lignocellulose Compounds. - 1 online resource (209 pages)

Source: Dissertations Abstracts International, Volume: 84-09, Section: B.

Thesis (Ph.D.)--North Carolina State University, 2022.

Includes bibliographical references

Complex polymers represent a challenge for environmental pollution as well as an opportunity for microbial catalyzed conversion to generate valorized chemicals. For example, black liquor, a by-product from paper milling, contains lignocellulose components; however, it is typically burned to generate steam energy for the paper pulping process. Here, we investigate the lignin and hemicellulose-derived compound biodegradation potential of bacterial strains isolated from carpenter bees when they are grown on black liquor or lignin. The isolates were identified as Streptomyces spp. by 16S rRNA and whole genome sequencing. Growth was assessed for the isolates cultured in minimal media with the lignocellulose constituents (cellulose, xylan, and lignin) or black liquor (pulping waste) added as the only source of carbon. Filter paper deconstruction, dye decolorization assays, and % lignin reduction assays were used to determine the cellulose, hemicellulose, and lignin degradation potential of the isolates, respectively. Streptomyces spp. strains 2-6 and 2-10 were able to decolorize azo, trimethylmethane, and thiazine dyes up to 60, 100, and 18%, respectively, after one week incubation without the addition of a reaction mediator. Cellulose deconstruction experiments showed degradation of up to 30% of the filter paper within 10 days. Growth on lignin revealed that Streptomyces sp. strain 2-6 could degrade up to 24% of the lignin mass within 30 days. Whole genome analysis of Streptomyces sp. 2-6 was employed to identify potential lignin and lignocellulose metabolism genes and associated pathway which are comparable with previously characterize lignindegrading Streptomyces spp. The potential of lignin deconstruction by Streptomyces sp. 2-6 was evaluated using Gel-Permeation Chromatography, and GC-MS was used to identify products generated when Streptomyces sp. 2-6 was culture on lignin and black liquor. This work demonstrated that Streptomyces sp. 2-6 can use low molecular weight lignin as a sole carbon source and that it promotes de-polymerization of high molecular weight lignin and produces organic acids with industrial relevance. Findings suggest the exploration of carpenter bee microbiota for the biodegradation and bioconversion of lignocellulosic derivatives.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798374431834Subjects--Topical Terms:

571472
Fungi.
Index Terms--Genre/Form:

542853
Electronic books.

Characterization of Bacterial Isolates from Carpenter Bees for Biodegradation of Lignocellulose Compounds.
LDR:03696nmm a2200397K 4500 001 2356313
005 20230612072304.5
006 m o d
007 cr mn ---uuuuu
008 241011s2022 xx obm 000 0 eng d
020 $a 9798374431834
035 $a (MiAaPQ)AAI30283627
035 $a (MiAaPQ)NCState_Univ18402040143
035 $a AAI30283627
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Cuebas-Irizarry, Mara Francheska. $3 3696793
245 1 0 $a Characterization of Bacterial Isolates from Carpenter Bees for Biodegradation of Lignocellulose Compounds.
264 0 $c 2022
300 $a 1 online resource (209 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 84-09, Section: B.
500 $a Advisor: Grunden, Amy M. ; Bruno-Barcena, Jose M.
502 $a Thesis (Ph.D.)--North Carolina State University, 2022.
504 $a Includes bibliographical references
520 $a Complex polymers represent a challenge for environmental pollution as well as an opportunity for microbial catalyzed conversion to generate valorized chemicals. For example, black liquor, a by-product from paper milling, contains lignocellulose components; however, it is typically burned to generate steam energy for the paper pulping process. Here, we investigate the lignin and hemicellulose-derived compound biodegradation potential of bacterial strains isolated from carpenter bees when they are grown on black liquor or lignin. The isolates were identified as Streptomyces spp. by 16S rRNA and whole genome sequencing. Growth was assessed for the isolates cultured in minimal media with the lignocellulose constituents (cellulose, xylan, and lignin) or black liquor (pulping waste) added as the only source of carbon. Filter paper deconstruction, dye decolorization assays, and % lignin reduction assays were used to determine the cellulose, hemicellulose, and lignin degradation potential of the isolates, respectively. Streptomyces spp. strains 2-6 and 2-10 were able to decolorize azo, trimethylmethane, and thiazine dyes up to 60, 100, and 18%, respectively, after one week incubation without the addition of a reaction mediator. Cellulose deconstruction experiments showed degradation of up to 30% of the filter paper within 10 days. Growth on lignin revealed that Streptomyces sp. strain 2-6 could degrade up to 24% of the lignin mass within 30 days. Whole genome analysis of Streptomyces sp. 2-6 was employed to identify potential lignin and lignocellulose metabolism genes and associated pathway which are comparable with previously characterize lignindegrading Streptomyces spp. The potential of lignin deconstruction by Streptomyces sp. 2-6 was evaluated using Gel-Permeation Chromatography, and GC-MS was used to identify products generated when Streptomyces sp. 2-6 was culture on lignin and black liquor. This work demonstrated that Streptomyces sp. 2-6 can use low molecular weight lignin as a sole carbon source and that it promotes de-polymerization of high molecular weight lignin and produces organic acids with industrial relevance. Findings suggest the exploration of carpenter bee microbiota for the biodegradation and bioconversion of lignocellulosic derivatives.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Fungi. $3 571472
650 4 $a Bioremediation. $3 677632
650 4 $a Pollutants. $3 551065
650 4 $a Biodiesel fuels. $3 587935
650 4 $a Lignin. $3 1097216
650 4 $a Bacteria. $3 550366
650 4 $a Biomass. $3 1013462
650 4 $a Metabolism. $3 541349
650 4 $a Liquor. $3 3683856
650 4 $a Effluents. $3 3559973
650 4 $a Apoptosis. $3 600650
650 4 $a Climate change. $2 bicssc $3 2079509
650 4 $a Ethanol. $3 1613481
650 4 $a Dyes. $3 3559972
650 4 $a By products. $3 3564729
650 4 $a Oxidation. $3 714629
650 4 $a Creeks & streams. $3 3685269
650 4 $a Cellulose. $3 588995
650 4 $a Carbon. $3 604057
650 4 $a Biodegradation. $3 705930
650 4 $a Alternative energy sources. $3 3561089
650 4 $a Soil contamination. $3 3557940
650 4 $a Enzymes. $3 520899
650 4 $a Metabolites. $3 683644
650 4 $a Alternative energy. $3 3436775
650 4 $a Aquatic sciences. $3 3174300
650 4 $a Cellular biology. $3 3172791
650 4 $a Energy. $3 876794
650 4 $a Soil sciences. $3 2122699
650 4 $a Textile research. $3 2153103
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0404
690 $a 0363
690 $a 0792
690 $a 0379
690 $a 0791
690 $a 0481
690 $a 0994
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a North Carolina State University. $3 1018772
773 0 $t Dissertations Abstracts International $g 84-09B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30283627 $z click for full text (PQDT)