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Label-Free Photoacoustic Microscopy for Biomedical and Point-of-Care Applications.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Label-Free Photoacoustic Microscopy for Biomedical and Point-of-Care Applications./
作者:	Li, Xiufeng.
面頁冊數:	1 online resource (115 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-05, Section: A.
Contained By:	Dissertations Abstracts International84-05A.
標題:	Cancer. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29704083click for full text (PQDT)
ISBN:	9798352977347

Label-Free Photoacoustic Microscopy for Biomedical and Point-of-Care Applications.
Li, Xiufeng.

Label-Free Photoacoustic Microscopy for Biomedical and Point-of-Care Applications. - 1 online resource (115 pages)

Source: Dissertations Abstracts International, Volume: 84-05, Section: A.

Thesis (Ph.D.)--Hong Kong University of Science and Technology (Hong Kong), 2022.

Includes bibliographical references

Photoacoustic tomography (PAT) is a promising imaging technique which utilizes ultrasonic waves induced by pulse light energy absorbed by intrinsic biomolecules, such as DNA/RNA and hemoglobin, to reconstruct images. Taking the advantage of endogenous optical absorption contrast, PAT can provide label-free structural, histological, functional, and metabolic images for clinical applications and basic studies. In this thesis, we propose several techniques to improve the performance of PAT and promote its applications in biomedical and point-of-care fields.With the high optical absorption of DNA/RNA at 266 nm, ultraviolet photoacoustic microscopy (UV-PAM) has been developed for cellular imaging, providing histological information for revealing organ structures and disease diagnosis. However, the cellular contrast might be low because of the high UV absorption of lipids and pigments into tissues, affecting the microstructure analysis and degrading the diagnostic accuracy. To improve the cellular contrast of UV-PAM, we first develop a tissue clearing-enhanced UV-PAM to remove lipids and pigments, reducing the background signals. The image contrast has been significantly improved and multilayers of cell nuclei can be obtained after tissue clearing. Then, we further develop a dual-modality imaging system with UV-PA and auto-fluorescence microscopy (uvPA-AFM) to achieve cellular contrast improvement without any tissue processing, promoting the clinical applications of UV-PAM in histological examination of various tissues. High-quality histological images can even be obtained by our system under low excitation energy and high pulse-to-pulse energy fluctuation.To overcome the costly and bulky lasers in conventional PAM systems for microvasculature imaging, we develop a high-speed high-resolution PAM system with a cost-effective and compact laser diode (LD). The theoretical resolution is first analyzed by consideration of the emitter size of LD, and then an optimized optical system is developed to reshape the LD beam, achieving a high lateral resolution of 4.8 μm. We then further develop a reflection-mode dualwavelength low-cost high-resolution LD-based PAM system, promoting its application in blood oxygenation imaging. Experimental results of in-vivo mouse ear imaging show that our system can provide high-resolution functional images. With the high performance, low cost, and compactness, our system has high potential in point-of-care applications.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798352977347Subjects--Topical Terms:

634186
Cancer.
Index Terms--Genre/Form:

542853
Electronic books.

Label-Free Photoacoustic Microscopy for Biomedical and Point-of-Care Applications.
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502 $a Thesis (Ph.D.)--Hong Kong University of Science and Technology (Hong Kong), 2022.
504 $a Includes bibliographical references
520 $a Photoacoustic tomography (PAT) is a promising imaging technique which utilizes ultrasonic waves induced by pulse light energy absorbed by intrinsic biomolecules, such as DNA/RNA and hemoglobin, to reconstruct images. Taking the advantage of endogenous optical absorption contrast, PAT can provide label-free structural, histological, functional, and metabolic images for clinical applications and basic studies. In this thesis, we propose several techniques to improve the performance of PAT and promote its applications in biomedical and point-of-care fields.With the high optical absorption of DNA/RNA at 266 nm, ultraviolet photoacoustic microscopy (UV-PAM) has been developed for cellular imaging, providing histological information for revealing organ structures and disease diagnosis. However, the cellular contrast might be low because of the high UV absorption of lipids and pigments into tissues, affecting the microstructure analysis and degrading the diagnostic accuracy. To improve the cellular contrast of UV-PAM, we first develop a tissue clearing-enhanced UV-PAM to remove lipids and pigments, reducing the background signals. The image contrast has been significantly improved and multilayers of cell nuclei can be obtained after tissue clearing. Then, we further develop a dual-modality imaging system with UV-PA and auto-fluorescence microscopy (uvPA-AFM) to achieve cellular contrast improvement without any tissue processing, promoting the clinical applications of UV-PAM in histological examination of various tissues. High-quality histological images can even be obtained by our system under low excitation energy and high pulse-to-pulse energy fluctuation.To overcome the costly and bulky lasers in conventional PAM systems for microvasculature imaging, we develop a high-speed high-resolution PAM system with a cost-effective and compact laser diode (LD). The theoretical resolution is first analyzed by consideration of the emitter size of LD, and then an optimized optical system is developed to reshape the LD beam, achieving a high lateral resolution of 4.8 μm. We then further develop a reflection-mode dualwavelength low-cost high-resolution LD-based PAM system, promoting its application in blood oxygenation imaging. Experimental results of in-vivo mouse ear imaging show that our system can provide high-resolution functional images. With the high performance, low cost, and compactness, our system has high potential in point-of-care applications.
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