東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Linked to FindBook

Google Book

Amazon

博客來

Development and Application of Function-Driven Chemical Biology Approaches to Characterize Chitinolysis in Soil Microbiomes.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Development and Application of Function-Driven Chemical Biology Approaches to Characterize Chitinolysis in Soil Microbiomes./
Author:	Zegeye, Elias Kebede.
Description:	1 online resource (172 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 84-05, Section: B.
Contained By:	Dissertations Abstracts International84-05B.
Subject:	Chemical engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29209951click for full text (PQDT)
ISBN:	9798357546173

Development and Application of Function-Driven Chemical Biology Approaches to Characterize Chitinolysis in Soil Microbiomes.
Zegeye, Elias Kebede.

Development and Application of Function-Driven Chemical Biology Approaches to Characterize Chitinolysis in Soil Microbiomes. - 1 online resource (172 pages)

Source: Dissertations Abstracts International, Volume: 84-05, Section: B.

Thesis (Ph.D.)--Washington State University, 2022.

Includes bibliographical references

Microbial catabolism of chitin plays fundamental roles in ecosystem functioning, yet a full, mechanistic understanding remains elusive. To gain a function-dependent understanding we integrated omics-based techniques with novel chemical biology approaches to directly characterize chitinase activity in a soil biosystem. Herein, the strategies of chemical biology and chemoproteomics, synthesis of activity-based probes, and techniques to measure enzymatic activities in a soil rhizosphere is introduced in Chapter One. A connection is made to show how these function-forward approaches can be applied in reduced complexity soil microbiomes. Chapter Two presents the synthesis of activity-based probes for chitinases and related glycoside hydrolase enzymes by modifying N-acetyl glucosamine and chitotriose. These probes were used to determine the active complement of chitinolytic enzymes produced over time by the soil bacterium Cellvibrio japonicus. Chapter Three discuss the development of stable and simple chitinolytic consortia from native soil. We investigated the successional dynamics of a native soil microbiome during long-term enrichment on chitin and N-acetyl glucosamine. We examined succession of the community in a structured soil and unstructured liquid environment at various initial species richness. We found that lower initial richness results in a more rapid convergence to community stability. The structured soil environment, rather than liquid, formed minimized chitinolytic communities best representative of a complex natural soil community. The results provided enhanced understanding about the succession, selection, and stabilization of a chitinolytic microbiome. Chapter Four discusses the application of chemical biology in a soil rhizosphere. We synthesized a fluorescence substrate, chitotriose-TokyoGreen, which is selectively triggered by active chitinase enzymes producing a fluorescence signal to spatially resolve chitinases. When proteins were extracted from a switchgrass rhizosphere using a nitrocellulose membrane and stained with chitotriose-TokyoGreen, chitinases were found to be primarily distributed along an area with supplemented chitin. Finally, Chapter Five provides a summary of the research, describes key scientific contributions, and describes future directions. Overall, our chemical biology approach provides a function-resolved molecular level insight to the roles chitinase and chitinolytic microbes play in soil nutrient cycling in the face of environmental changes.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798357546173Subjects--Topical Terms:

560457
Chemical engineering.
Subjects--Index Terms:

Activity-based probeIndex Terms--Genre/Form:

542853
Electronic books.

Development and Application of Function-Driven Chemical Biology Approaches to Characterize Chitinolysis in Soil Microbiomes.
LDR:03929nmm a2200385K 4500 001 2354260
005 20230403071202.5
006 m o d
007 cr mn ---uuuuu
008 241011s2022 xx obm 000 0 eng d
020 $a 9798357546173
035 $a (MiAaPQ)AAI29209951
035 $a AAI29209951
040 $a MiAaPQ $b eng $c MiAaPQ $d NTU
100 1 $a Zegeye, Elias Kebede. $3 3694609
245 1 0 $a Development and Application of Function-Driven Chemical Biology Approaches to Characterize Chitinolysis in Soil Microbiomes.
264 0 $c 2022
300 $a 1 online resource (172 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Dissertations Abstracts International, Volume: 84-05, Section: B.
500 $a Advisor: Wright, Aaron.
502 $a Thesis (Ph.D.)--Washington State University, 2022.
504 $a Includes bibliographical references
520 $a Microbial catabolism of chitin plays fundamental roles in ecosystem functioning, yet a full, mechanistic understanding remains elusive. To gain a function-dependent understanding we integrated omics-based techniques with novel chemical biology approaches to directly characterize chitinase activity in a soil biosystem. Herein, the strategies of chemical biology and chemoproteomics, synthesis of activity-based probes, and techniques to measure enzymatic activities in a soil rhizosphere is introduced in Chapter One. A connection is made to show how these function-forward approaches can be applied in reduced complexity soil microbiomes. Chapter Two presents the synthesis of activity-based probes for chitinases and related glycoside hydrolase enzymes by modifying N-acetyl glucosamine and chitotriose. These probes were used to determine the active complement of chitinolytic enzymes produced over time by the soil bacterium Cellvibrio japonicus. Chapter Three discuss the development of stable and simple chitinolytic consortia from native soil. We investigated the successional dynamics of a native soil microbiome during long-term enrichment on chitin and N-acetyl glucosamine. We examined succession of the community in a structured soil and unstructured liquid environment at various initial species richness. We found that lower initial richness results in a more rapid convergence to community stability. The structured soil environment, rather than liquid, formed minimized chitinolytic communities best representative of a complex natural soil community. The results provided enhanced understanding about the succession, selection, and stabilization of a chitinolytic microbiome. Chapter Four discusses the application of chemical biology in a soil rhizosphere. We synthesized a fluorescence substrate, chitotriose-TokyoGreen, which is selectively triggered by active chitinase enzymes producing a fluorescence signal to spatially resolve chitinases. When proteins were extracted from a switchgrass rhizosphere using a nitrocellulose membrane and stained with chitotriose-TokyoGreen, chitinases were found to be primarily distributed along an area with supplemented chitin. Finally, Chapter Five provides a summary of the research, describes key scientific contributions, and describes future directions. Overall, our chemical biology approach provides a function-resolved molecular level insight to the roles chitinase and chitinolytic microbes play in soil nutrient cycling in the face of environmental changes.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
650 4 $a Chemical engineering. $3 560457
650 4 $a Bioengineering. $3 657580
653 $a Activity-based probe
653 $a Chemical biology
653 $a Chemical proteomics
653 $a Chitin
653 $a Soil microbiomes
655 7 $a Electronic books. $2 lcsh $3 542853
690 $a 0542
690 $a 0202
710 2 $a ProQuest Information and Learning Co. $3 783688
710 2 $a Washington State University. $b School of Chemical Engineering and Bioengineering. $3 3694610
773 0 $t Dissertations Abstracts International $g 84-05B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29209951 $z click for full text (PQDT)