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Impact of Electrically Stimulated Eccentric Contraction Training on Innervation and Cellular Phenotype in Injured Muscles.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Impact of Electrically Stimulated Eccentric Contraction Training on Innervation and Cellular Phenotype in Injured Muscles./
Author:	Chauvin, Hannah Michelle.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2022,
Description:	53 p.
Notes:	Source: Masters Abstracts International, Volume: 83-12.
Contained By:	Masters Abstracts International83-12.
Subject:	Biomedical engineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29206635
ISBN:	9798438797487

Impact of Electrically Stimulated Eccentric Contraction Training on Innervation and Cellular Phenotype in Injured Muscles.
Chauvin, Hannah Michelle.

Impact of Electrically Stimulated Eccentric Contraction Training on Innervation and Cellular Phenotype in Injured Muscles. - Ann Arbor : ProQuest Dissertations & Theses, 2022 - 53 p.

Source: Masters Abstracts International, Volume: 83-12.

Thesis (M.S.)--Saint Louis University, 2022.

This item must not be sold to any third party vendors.

Volumetric muscle loss (VML) is a traumatic injury that causes life-long dysfunction and disability. To rehabilitate the injured skeletal muscle, we developed a regimen consisting of electrically stimulated eccentric contraction training (EST). A VML defect (~20% by mass) was created in the tibialis anterior muscle of adult male Lewis rats. After a 2-week healing period, EST took place twice a week for one or four weeks. Each session consisted of 20 muscle contractions at 50, 100, or 150 Hz frequency (n=6-9). Contractions elicited at higher frequencies were expected to increase the degree of mechanical loading on the injured muscles. EST primarily affected the phenotypic expression of inflammatory cells. While the total number of macrophages (CD68+) remained unaltered across treatment groups, the ratio of M1 (iNOS+)- to M2 (Arginase+)-like macrophages was significantly lower in the 100 and 150 Hz groups, suggesting a heightened immunomodulatory response. Surprisingly, myoblast, mesenchymal stem cell, fibroadipogenic progenitor cell, and pericyte populations did not appear to be significantly impacted by EST. However, as suggested by macrophage analysis, it is possible that EST elicits changes in phenotypic expression of stem cells in while keeping overall populations consistent. Furthermore, EST implemented at 150 Hz increased post-synaptic acetylcholine receptor (AchR) clusters (aBTX+) in myofibers without pre-synaptic peripheral nerve axons (neurofilament+). These results indicate that EST enhances immunomodulation and promotes readiness for myofiber innervation. Together these findings may provide the basis for improved muscle mass and function observed with EST.

ISBN: 9798438797487Subjects--Topical Terms:

535387
Biomedical engineering.
Subjects--Index Terms:

Biomedical engineering

Impact of Electrically Stimulated Eccentric Contraction Training on Innervation and Cellular Phenotype in Injured Muscles.
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520 $a Volumetric muscle loss (VML) is a traumatic injury that causes life-long dysfunction and disability. To rehabilitate the injured skeletal muscle, we developed a regimen consisting of electrically stimulated eccentric contraction training (EST). A VML defect (~20% by mass) was created in the tibialis anterior muscle of adult male Lewis rats. After a 2-week healing period, EST took place twice a week for one or four weeks. Each session consisted of 20 muscle contractions at 50, 100, or 150 Hz frequency (n=6-9). Contractions elicited at higher frequencies were expected to increase the degree of mechanical loading on the injured muscles. EST primarily affected the phenotypic expression of inflammatory cells. While the total number of macrophages (CD68+) remained unaltered across treatment groups, the ratio of M1 (iNOS+)- to M2 (Arginase+)-like macrophages was significantly lower in the 100 and 150 Hz groups, suggesting a heightened immunomodulatory response. Surprisingly, myoblast, mesenchymal stem cell, fibroadipogenic progenitor cell, and pericyte populations did not appear to be significantly impacted by EST. However, as suggested by macrophage analysis, it is possible that EST elicits changes in phenotypic expression of stem cells in while keeping overall populations consistent. Furthermore, EST implemented at 150 Hz increased post-synaptic acetylcholine receptor (AchR) clusters (aBTX+) in myofibers without pre-synaptic peripheral nerve axons (neurofilament+). These results indicate that EST enhances immunomodulation and promotes readiness for myofiber innervation. Together these findings may provide the basis for improved muscle mass and function observed with EST.
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