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A functional analysis of Commelina yellow mottle virus gene products.

Record Type:	Electronic resources : Monograph/item
Title/Author:	A functional analysis of Commelina yellow mottle virus gene products./
Author:	Cheng, Chiu-Ping.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 1996,
Description:	116 p.
Notes:	Source: Dissertations Abstracts International, Volume: 58-03, Section: B.
Contained By:	Dissertations Abstracts International58-03B.
Subject:	Plant pathology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9632360
ISBN:	9798691259296

A functional analysis of Commelina yellow mottle virus gene products.
Cheng, Chiu-Ping.

A functional analysis of Commelina yellow mottle virus gene products. - Ann Arbor : ProQuest Dissertations & Theses, 1996 - 116 p.

Source: Dissertations Abstracts International, Volume: 58-03, Section: B.

Thesis (Ph.D.)--University of Minnesota, 1996.

This item must not be sold to any third party vendors.

Antibodies that were prepared against bacterially-expressed Commelina yellow mottle badnavirus (CoYMV) proteins and purified virions were used to determine the localization of the viral proteins. By using these antisera, it was also possible to confirm that the CoYMV coat protein is encoded in ORF III. In addition to the two major forms of the coat protein (37 kDa and 39 kDa), several high molecular weight virus-specific proteins were detected. These proteins are possible ORF III polyprotein processing intermediates and might be associated with "immature" virions. As predicted by the genomic sequence, a 20 kDa virus-specific protein was detected by an antiserum raised against the C-terminus of the putative ORF I protein and this protein was shown to be associated with "immature" virions and with plant component(s). An antiserum raised against the putative ORF II gene product detected a 15 kDa virus-specific protein which was associated with both "mature" and "immature" virions and was found to be sensitive to some protease(s) that co-purified with the virions. Virion-containing tubules were found in cell-free extracts prepared from CoYMV-infected Commelina diffusa. Similar virion-containing tubules were observed within or passing through both cell wall thickenings and cell wall protrusions, suggesting that the tubules play a role in cell-to-cell movement of virions. An ORF I mutant which contained a 13 amino acid deletion exhibited phenotypes of reduced mealybug transmissibility which could be improved by serial mealybug transmissions, delayed symptom appearance which was unaltered during the serial mealybug transmissions, a reduced amount of detectable ORF I protein and slower systemic movement in plants compared to the wild-type virus. These results suggested a possible role of the ORF I protein in mealybug transmission of CoYMV and in symptom development. A new transposon-mediated mutagenesis method was developed and applied to the CoYMV genome to generate random, non-polar mutations for elucidating the functions of the viral gene products. The possible functions of these viral proteins are discussed.

ISBN: 9798691259296Subjects--Topical Terms:

3174872
Plant pathology.

A functional analysis of Commelina yellow mottle virus gene products.
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020 $a 9798691259296
035 $a (MiAaPQ)AAI9632360
035 $a AAI9632360
040 $a MiAaPQ $c MiAaPQ
100 1 $a Cheng, Chiu-Ping. $3 3688900
245 1 0 $a A functional analysis of Commelina yellow mottle virus gene products.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 1996
300 $a 116 p.
500 $a Source: Dissertations Abstracts International, Volume: 58-03, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Olszewski, Neil E.
502 $a Thesis (Ph.D.)--University of Minnesota, 1996.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a Antibodies that were prepared against bacterially-expressed Commelina yellow mottle badnavirus (CoYMV) proteins and purified virions were used to determine the localization of the viral proteins. By using these antisera, it was also possible to confirm that the CoYMV coat protein is encoded in ORF III. In addition to the two major forms of the coat protein (37 kDa and 39 kDa), several high molecular weight virus-specific proteins were detected. These proteins are possible ORF III polyprotein processing intermediates and might be associated with "immature" virions. As predicted by the genomic sequence, a 20 kDa virus-specific protein was detected by an antiserum raised against the C-terminus of the putative ORF I protein and this protein was shown to be associated with "immature" virions and with plant component(s). An antiserum raised against the putative ORF II gene product detected a 15 kDa virus-specific protein which was associated with both "mature" and "immature" virions and was found to be sensitive to some protease(s) that co-purified with the virions. Virion-containing tubules were found in cell-free extracts prepared from CoYMV-infected Commelina diffusa. Similar virion-containing tubules were observed within or passing through both cell wall thickenings and cell wall protrusions, suggesting that the tubules play a role in cell-to-cell movement of virions. An ORF I mutant which contained a 13 amino acid deletion exhibited phenotypes of reduced mealybug transmissibility which could be improved by serial mealybug transmissions, delayed symptom appearance which was unaltered during the serial mealybug transmissions, a reduced amount of detectable ORF I protein and slower systemic movement in plants compared to the wild-type virus. These results suggested a possible role of the ORF I protein in mealybug transmission of CoYMV and in symptom development. A new transposon-mediated mutagenesis method was developed and applied to the CoYMV genome to generate random, non-polar mutations for elucidating the functions of the viral gene products. The possible functions of these viral proteins are discussed.
590 $a School code: 0130.
650 4 $a Plant pathology. $3 3174872
650 4 $a Molecular biology. $3 517296
650 4 $a Microbiology. $3 536250
690 $a 0480
690 $a 0307
690 $a 0410
710 2 $a University of Minnesota. $3 676231
773 0 $t Dissertations Abstracts International $g 58-03B.
790 $a 0130
791 $a Ph.D.
792 $a 1996
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9632360