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Studies of the pollen-determinant of self -incompatibility and identification of candidate pistil factors for compatible pollination in the crucifer family.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Studies of the pollen-determinant of self -incompatibility and identification of candidate pistil factors for compatible pollination in the crucifer family./
作者:	Tung, Chih-Wei.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2004,
面頁冊數:	161 p.
附註:	Source: Dissertations Abstracts International, Volume: 66-06, Section: B.
Contained By:	Dissertations Abstracts International66-06B.
標題:	Botany. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3140829
ISBN:	9780496879694

Studies of the pollen-determinant of self -incompatibility and identification of candidate pistil factors for compatible pollination in the crucifer family.
Tung, Chih-Wei.

Studies of the pollen-determinant of self -incompatibility and identification of candidate pistil factors for compatible pollination in the crucifer family. - Ann Arbor : ProQuest Dissertations & Theses, 2004 - 161 p.

Source: Dissertations Abstracts International, Volume: 66-06, Section: B.

Thesis (Ph.D.)--Cornell University, 2004.

This item must not be sold to any third party vendors.

In crucifers, sporophytic control of pollen self-incompatibility (SI) phenotype and the occurrence of dominance/recessive interactions among S haplotypes in pollen were not understood at the molecular level. With the isolation of SCR (S-locus Cys-rich), the male determinant of SI, it became possible to test two major models of sporophytic control of SI that were proposed in the 1970s and late 1990s. In an attempt to test these models, I used two approaches. A transgenic approach, in which SCR was expressed in Brassica oleracea either sporophytically under the control of a tapetum-specific promoter or gametophytically under the control of a pollen-specific promoter, was inconclusive. However, another approach, in which Arabidopsis lyrata SCR alleles exhibiting inherent differences in expression pattern were analyzed by in situ hybridization, demonstrated that tapetal expression of SCR is sufficient to confer pollen SI specificity. This analysis also showed that, in heterozygotes, recessive SCR alleles are silenced in the presence of a dominant SCR allele, thus providing a molecular explanation for dominant/recessive interactions of S haplotypes in anthers. Successful fertilization in flowering plants is dependent on highly specific pollen-pistil interactions beginning at the stigma epidermis and culminating in fertilization of ovules in the ovary. Very little is known about the mechanism and regulation of pollen adhesion, pollen hydration, and pollen tube growth and guidance within the pistil. To identify genes having potential roles in these processes, I used a method that combines genetic ablation of cells of the stigma epidermis and transmitting tract with genome-wide transcript profiling. This analysis identified 115 and 34 genes predicted to be express specifically in stigma epidermis and in transmitting tract, respectively. The identity, validation, and initial characterization of these transcripts are presented.

ISBN: 9780496879694Subjects--Topical Terms:

516217
Botany.
Subjects--Index Terms:

Crucifer

Studies of the pollen-determinant of self -incompatibility and identification of candidate pistil factors for compatible pollination in the crucifer family.
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520 $a In crucifers, sporophytic control of pollen self-incompatibility (SI) phenotype and the occurrence of dominance/recessive interactions among S haplotypes in pollen were not understood at the molecular level. With the isolation of SCR (S-locus Cys-rich), the male determinant of SI, it became possible to test two major models of sporophytic control of SI that were proposed in the 1970s and late 1990s. In an attempt to test these models, I used two approaches. A transgenic approach, in which SCR was expressed in Brassica oleracea either sporophytically under the control of a tapetum-specific promoter or gametophytically under the control of a pollen-specific promoter, was inconclusive. However, another approach, in which Arabidopsis lyrata SCR alleles exhibiting inherent differences in expression pattern were analyzed by in situ hybridization, demonstrated that tapetal expression of SCR is sufficient to confer pollen SI specificity. This analysis also showed that, in heterozygotes, recessive SCR alleles are silenced in the presence of a dominant SCR allele, thus providing a molecular explanation for dominant/recessive interactions of S haplotypes in anthers. Successful fertilization in flowering plants is dependent on highly specific pollen-pistil interactions beginning at the stigma epidermis and culminating in fertilization of ovules in the ovary. Very little is known about the mechanism and regulation of pollen adhesion, pollen hydration, and pollen tube growth and guidance within the pistil. To identify genes having potential roles in these processes, I used a method that combines genetic ablation of cells of the stigma epidermis and transmitting tract with genome-wide transcript profiling. This analysis identified 115 and 34 genes predicted to be express specifically in stigma epidermis and in transmitting tract, respectively. The identity, validation, and initial characterization of these transcripts are presented.
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