東華大學圖書館 |

Modeling Virus Infection and Pathogenesis Using Stem Cell Derived Systems.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Modeling Virus Infection and Pathogenesis Using Stem Cell Derived Systems./
作者:	Cheng, Yichen.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	128 p.
附註:	Source: Dissertations Abstracts International, Volume: 83-08, Section: B.
Contained By:	Dissertations Abstracts International83-08B.
標題:	Biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28772951
ISBN:	9798780618805

Modeling Virus Infection and Pathogenesis Using Stem Cell Derived Systems.
Cheng, Yichen.

Modeling Virus Infection and Pathogenesis Using Stem Cell Derived Systems. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 128 p.

Source: Dissertations Abstracts International, Volume: 83-08, Section: B.

Thesis (Ph.D.)--The Florida State University, 2021.

This item must not be sold to any third party vendors.

Zika virus (ZIKV) is a re-emerging mosquito-borne flavivirus that has been linked to severe neurological disorders such as microcephaly in newborns as well as Guillain-Barre syndrome in adults. It is closely related to other flaviviruses such as Dengue virus (DENV), West Nile virus and Yellow Fever virus with high similarities in structure and genomic organization. However, the tissue tropism after systematic infection and the clinical outcomes varies significantly between each species. Among its family members, ZIKV is distinct regarding its ability to infiltrate multiple immune-privileged sites, especially the brain, as well as its neurotropic nature to cause neurological defects in the central nervous system (CNS). However, the detailed molecular mechanism(s) by which it gains access to the brain and impairs development and function are not yet fully elucidated.To first understand how ZIKV access the brain, which is typically protected by blood-brain barrier (BBB), we utilized induced human brain microvascular endothelial cells (iBMECs) derived from pluripotent stem cells (PSCs) to study virus-BBB interactions. These iBMECs exhibited functional and physiological BBB properties and faithfully recapitulated the in vivo neuroinvasiveness of both alpha- and flavi-viruses. We further developed a BBB model consisting of cocultured iBMECs and astrocytes in transwell setups. This model exhibited high trans-endothelial electrical resistance (TEER) value and selectively blocked non-neurotropic flaviviruses from crossing the barrier. Gene profiling identified that iBMECs constitutively express an interferon-stimulated gene, IFITM1, which contributes to the selective restriction of non-neurotropic flaviviruses. We further investigated the interactions between ZIKV and other physiological barriers including blood-testis barrier (BTB) and blood-retinal barrier (BRB). Barrier cells from BTB and BRB also intrinsically expressed IFITM proteins that contribute to their viral resistance. We discovered that the membrane localization of IFITM1 as well as the structure of DENV prM-E region both contribute to the selective restriction of DENV. Our application of this renewable human iPSC-based model for studying virus-BBB interactions revealed dual mechanism for human physiological barriers to restrict viral infection and highlights the ability of ZIKV to specifically bypass this restriction as a mechanism for it to gain access to these immune-privileged sites.We next focused on understanding ZIKV pathogenesis in brain after breaching the BBB. Astrocytes are the major cell population in the brain and one of the first cell types to be targeted by virus infection. While extensive studies have examined the role of ZIKV infection in human neural progenitor cells, the role of astrocytes has yet to be fully examined. Here, we utilized iPSC-derived astrocytes and neurons to study ZIKV infection in CNS. Both stem cell-derived and primary astrocytes were highly permissive to ZIKV infection and support production of infectious ZIKV virions. Increased caspase-3 activity and decreased ATPase content were observed in ZIKV-infected astrocytes, suggesting ZIKV infection significantly induced cell death in astrocytes. Global transcriptome analysis revealed that ZIKV triggered antiviral response as well as cytokines/chemokines production in infected astrocytes. Specifically, we identified two important cytokines involved in synaptic maintenance and neurogenesis, IL-6 and CXCL10, in supernatants of ZIKV-infected astrocytes. Treating the iPSC-derived neurons with conditioned medium from ZIKV-infected astrocytes drastically decreased the synaptic density of mature neurons, and this reduction was rescued by supplementary treatment of anti-IL6 antibody. These discoveries revealed the non-cell autonomous effect of ZIKV-infected astrocytes on neuronal survival and synaptogenesis that can be a contributive mechanism for ZIKV pathogenesis in CNS.Collectively, by using iPSC-derived iBMECs, astrocytes and neurons, we were able to effectively model virus infection in these physiologically relevant cell types. We identified two mechanisms, applied either by the host cells or the viruses, that donate to ZIKV pathogenesis. Our study highlights ZIKV's ability to bypass the cell-autonomous antiviral immunity conferred by the physiological blood-tissue barriers and disrupt neuronal synaptic formation in a non-cell autonomous manner conferred by infected astrocytes.

ISBN: 9798780618805Subjects--Topical Terms:

522710
Biology.
Subjects--Index Terms:

Blood brain barrier

Modeling Virus Infection and Pathogenesis Using Stem Cell Derived Systems.
LDR:05666nmm a2200373 4500 001 2349420
005 20220920134311.5
008 241004s2021 ||||||||||||||||| ||eng d
020 $a 9798780618805
035 $a (MiAaPQ)AAI28772951
035 $a AAI28772951
040 $a MiAaPQ $c MiAaPQ
100 1 $a Cheng, Yichen. $0 (orcid)0000-0003-3127-0102 $3 3688832
245 1 0 $a Modeling Virus Infection and Pathogenesis Using Stem Cell Derived Systems.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2021
300 $a 128 p.
500 $a Source: Dissertations Abstracts International, Volume: 83-08, Section: B.
500 $a Advisor: Tang, Hengli.
502 $a Thesis (Ph.D.)--The Florida State University, 2021.
506 $a This item must not be sold to any third party vendors.
520 $a Zika virus (ZIKV) is a re-emerging mosquito-borne flavivirus that has been linked to severe neurological disorders such as microcephaly in newborns as well as Guillain-Barre syndrome in adults. It is closely related to other flaviviruses such as Dengue virus (DENV), West Nile virus and Yellow Fever virus with high similarities in structure and genomic organization. However, the tissue tropism after systematic infection and the clinical outcomes varies significantly between each species. Among its family members, ZIKV is distinct regarding its ability to infiltrate multiple immune-privileged sites, especially the brain, as well as its neurotropic nature to cause neurological defects in the central nervous system (CNS). However, the detailed molecular mechanism(s) by which it gains access to the brain and impairs development and function are not yet fully elucidated.To first understand how ZIKV access the brain, which is typically protected by blood-brain barrier (BBB), we utilized induced human brain microvascular endothelial cells (iBMECs) derived from pluripotent stem cells (PSCs) to study virus-BBB interactions. These iBMECs exhibited functional and physiological BBB properties and faithfully recapitulated the in vivo neuroinvasiveness of both alpha- and flavi-viruses. We further developed a BBB model consisting of cocultured iBMECs and astrocytes in transwell setups. This model exhibited high trans-endothelial electrical resistance (TEER) value and selectively blocked non-neurotropic flaviviruses from crossing the barrier. Gene profiling identified that iBMECs constitutively express an interferon-stimulated gene, IFITM1, which contributes to the selective restriction of non-neurotropic flaviviruses. We further investigated the interactions between ZIKV and other physiological barriers including blood-testis barrier (BTB) and blood-retinal barrier (BRB). Barrier cells from BTB and BRB also intrinsically expressed IFITM proteins that contribute to their viral resistance. We discovered that the membrane localization of IFITM1 as well as the structure of DENV prM-E region both contribute to the selective restriction of DENV. Our application of this renewable human iPSC-based model for studying virus-BBB interactions revealed dual mechanism for human physiological barriers to restrict viral infection and highlights the ability of ZIKV to specifically bypass this restriction as a mechanism for it to gain access to these immune-privileged sites.We next focused on understanding ZIKV pathogenesis in brain after breaching the BBB. Astrocytes are the major cell population in the brain and one of the first cell types to be targeted by virus infection. While extensive studies have examined the role of ZIKV infection in human neural progenitor cells, the role of astrocytes has yet to be fully examined. Here, we utilized iPSC-derived astrocytes and neurons to study ZIKV infection in CNS. Both stem cell-derived and primary astrocytes were highly permissive to ZIKV infection and support production of infectious ZIKV virions. Increased caspase-3 activity and decreased ATPase content were observed in ZIKV-infected astrocytes, suggesting ZIKV infection significantly induced cell death in astrocytes. Global transcriptome analysis revealed that ZIKV triggered antiviral response as well as cytokines/chemokines production in infected astrocytes. Specifically, we identified two important cytokines involved in synaptic maintenance and neurogenesis, IL-6 and CXCL10, in supernatants of ZIKV-infected astrocytes. Treating the iPSC-derived neurons with conditioned medium from ZIKV-infected astrocytes drastically decreased the synaptic density of mature neurons, and this reduction was rescued by supplementary treatment of anti-IL6 antibody. These discoveries revealed the non-cell autonomous effect of ZIKV-infected astrocytes on neuronal survival and synaptogenesis that can be a contributive mechanism for ZIKV pathogenesis in CNS.Collectively, by using iPSC-derived iBMECs, astrocytes and neurons, we were able to effectively model virus infection in these physiologically relevant cell types. We identified two mechanisms, applied either by the host cells or the viruses, that donate to ZIKV pathogenesis. Our study highlights ZIKV's ability to bypass the cell-autonomous antiviral immunity conferred by the physiological blood-tissue barriers and disrupt neuronal synaptic formation in a non-cell autonomous manner conferred by infected astrocytes.
590 $a School code: 0071.
650 4 $a Biology. $3 522710
650 4 $a Cellular biology. $3 3172791
650 4 $a Virology. $3 642304
653 $a Blood brain barrier
653 $a Blood tissue barrier
653 $a Neurotropic virus
653 $a Virus-host interaction
653 $a Zika virus
690 $a 0306
690 $a 0379
690 $a 0720
710 2 $a The Florida State University. $b Biological Science. $3 3182932
773 0 $t Dissertations Abstracts International $g 83-08B.
790 $a 0071
791 $a Ph.D.
792 $a 2021
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28772951