東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

Investigating Gene Expression Effects of Neuregulin-1 (NRG-1) and its Influence on Response to Inflammatory Stimulation with LPS in Microglial Cells In Vitro.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Investigating Gene Expression Effects of Neuregulin-1 (NRG-1) and its Influence on Response to Inflammatory Stimulation with LPS in Microglial Cells In Vitro./
作者:	Augello, Catherine Jean.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2022,
面頁冊數:	172 p.
附註:	Source: Dissertations Abstracts International, Volume: 83-11, Section: B.
Contained By:	Dissertations Abstracts International83-11B.
標題:	Bioengineering. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28721146
ISBN:	9798426818736

Investigating Gene Expression Effects of Neuregulin-1 (NRG-1) and its Influence on Response to Inflammatory Stimulation with LPS in Microglial Cells In Vitro.
Augello, Catherine Jean.

Investigating Gene Expression Effects of Neuregulin-1 (NRG-1) and its Influence on Response to Inflammatory Stimulation with LPS in Microglial Cells In Vitro. - Ann Arbor : ProQuest Dissertations & Theses, 2022 - 172 p.

Source: Dissertations Abstracts International, Volume: 83-11, Section: B.

Thesis (Ph.D.)--University of California, Riverside, 2022.

This item must not be sold to any third party vendors.

Neuregulin-1 (NRG-1) is a potent, endogenous growth factor that has shown promise in treating pathologies with a neuroinflammatory component, but the mechanism through which NRG-1 treatment affects inflammation remains an area of investigation. Studies using NRG-1 as a therapeutic agent in stroke models have indicated that it has neuro-protective and anti-inflammatory properties. In this work we evaluate the role NRG-1 plays in altering inflammatory activation, especially in the context of neuropathology. We examine the effects of NRG-1 treatment on inflammation through in vitro application of NRG-1 to microglia, the main immune cell of the central nervous system (CNS).To execute this work we utilized the high through-put NanoString technology to track gene expression changes and to identify the most prominent features of NRG-1-induced alterations in the inflammatory state of activated SIM-A9 microglia. Stimulation with pro-inflammatory LPS and anti-inflammatory IL-4 were used as benchmarks of canonical microglial activation states to which NRG-1-induced changes were compared. The most compelling results from this work are that NRG-1 enhances NF-kB and apoptosis pathways that are triggered by pro-inflammatory stimulus and are critical to cell survival. NF-kB influences microglial inflammatory states by tweaking cell sensitivity to apoptotic signaling and increasing gene expression within survival pathways. Specifically, we found that NRG-1 pushes NF-kB signaling towards the non-canonical arm which results in increased expression of genes that encourage immune cell survival. This finding indicates a potentially novel mechanism through which NRG-1 mediates anti-inflammatory outcomes.

ISBN: 9798426818736Subjects--Topical Terms:

657580
Bioengineering.
Subjects--Index Terms:

Gene expression

Investigating Gene Expression Effects of Neuregulin-1 (NRG-1) and its Influence on Response to Inflammatory Stimulation with LPS in Microglial Cells In Vitro.
LDR:02964nmm a2200373 4500 001 2348910
005 20220920134619.5
008 241004s2022 ||||||||||||||||| ||eng d
020 $a 9798426818736
035 $a (MiAaPQ)AAI28721146
035 $a AAI28721146
040 $a MiAaPQ $c MiAaPQ
100 1 $a Augello, Catherine Jean. $3 3688294
245 1 0 $a Investigating Gene Expression Effects of Neuregulin-1 (NRG-1) and its Influence on Response to Inflammatory Stimulation with LPS in Microglial Cells In Vitro.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2022
300 $a 172 p.
500 $a Source: Dissertations Abstracts International, Volume: 83-11, Section: B.
500 $a Advisor: Ford, Byron.
502 $a Thesis (Ph.D.)--University of California, Riverside, 2022.
506 $a This item must not be sold to any third party vendors.
520 $a Neuregulin-1 (NRG-1) is a potent, endogenous growth factor that has shown promise in treating pathologies with a neuroinflammatory component, but the mechanism through which NRG-1 treatment affects inflammation remains an area of investigation. Studies using NRG-1 as a therapeutic agent in stroke models have indicated that it has neuro-protective and anti-inflammatory properties. In this work we evaluate the role NRG-1 plays in altering inflammatory activation, especially in the context of neuropathology. We examine the effects of NRG-1 treatment on inflammation through in vitro application of NRG-1 to microglia, the main immune cell of the central nervous system (CNS).To execute this work we utilized the high through-put NanoString technology to track gene expression changes and to identify the most prominent features of NRG-1-induced alterations in the inflammatory state of activated SIM-A9 microglia. Stimulation with pro-inflammatory LPS and anti-inflammatory IL-4 were used as benchmarks of canonical microglial activation states to which NRG-1-induced changes were compared. The most compelling results from this work are that NRG-1 enhances NF-kB and apoptosis pathways that are triggered by pro-inflammatory stimulus and are critical to cell survival. NF-kB influences microglial inflammatory states by tweaking cell sensitivity to apoptotic signaling and increasing gene expression within survival pathways. Specifically, we found that NRG-1 pushes NF-kB signaling towards the non-canonical arm which results in increased expression of genes that encourage immune cell survival. This finding indicates a potentially novel mechanism through which NRG-1 mediates anti-inflammatory outcomes.
590 $a School code: 0032.
650 4 $a Bioengineering. $3 657580
650 4 $a Neurosciences. $3 588700
650 4 $a Bioinformatics. $3 553671
653 $a Gene expression
653 $a Cytokine interleukin
653 $a Pro-inflammatory stimulus
653 $a Microglia
653 $a Neuregulin
690 $a 0202
690 $a 0317
690 $a 0715
710 2 $a University of California, Riverside. $b Bioengineering. $3 3174138
773 0 $t Dissertations Abstracts International $g 83-11B.
790 $a 0032
791 $a Ph.D.
792 $a 2022
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28721146