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Programming the Surface of Probiotics to Mitigate Bacterial Toxin-Mediated DNA Damage.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Programming the Surface of Probiotics to Mitigate Bacterial Toxin-Mediated DNA Damage./
Author:	Zhang, Songqi.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2022,
Description:	20 p.
Notes:	Source: Masters Abstracts International, Volume: 83-12.
Contained By:	Masters Abstracts International83-12.
Subject:	Bioengineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29168925
ISBN:	9798438767732

Programming the Surface of Probiotics to Mitigate Bacterial Toxin-Mediated DNA Damage.
Zhang, Songqi.

Programming the Surface of Probiotics to Mitigate Bacterial Toxin-Mediated DNA Damage. - Ann Arbor : ProQuest Dissertations & Theses, 2022 - 20 p.

Source: Masters Abstracts International, Volume: 83-12.

Thesis (M.S.)--Northeastern University, 2022.

This item must not be sold to any third party vendors.

Colorectal cancer (CRC) is the third most common kind of cancer globally. As a result, CRC prevention is critical for today's population. Previous research discovered that certain bacterial toxins could trigger DNA double-strand breaks in 66.7 percent of individuals with colorectal cancer. To inhibit the DNA damage in host cells, we engineered probiotics to release anti-toxin proteins, a mechanism by which toxin-expressing bacteria protect themselves from DNA damage. Because toxin-induced DNA damage requires cell-cell interaction, we effectively demonstrated the presence of anti-toxin proteins on the surface of E. coli using flow cytometry in this experiment. The results indicated that the anti-toxin N terminal display is distinct from the C terminal display. Although it cannot be detected using flow cytometry, it can be recognized using a western blot. Additionally, coculturing with HeLa cells demonstrated that E. coli generating toxin can induce DNA double-strand breaks and that E. coli expressing the anti-toxin can mitigate the DNA damage induced by the toxin.

ISBN: 9798438767732Subjects--Topical Terms:

657580
Bioengineering.
Subjects--Index Terms:

Clbs

Programming the Surface of Probiotics to Mitigate Bacterial Toxin-Mediated DNA Damage.
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245 1 0 $a Programming the Surface of Probiotics to Mitigate Bacterial Toxin-Mediated DNA Damage.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2022
300 $a 20 p.
500 $a Source: Masters Abstracts International, Volume: 83-12.
500 $a Advisor: Li, Jiahe.
502 $a Thesis (M.S.)--Northeastern University, 2022.
506 $a This item must not be sold to any third party vendors.
520 $a Colorectal cancer (CRC) is the third most common kind of cancer globally. As a result, CRC prevention is critical for today's population. Previous research discovered that certain bacterial toxins could trigger DNA double-strand breaks in 66.7 percent of individuals with colorectal cancer. To inhibit the DNA damage in host cells, we engineered probiotics to release anti-toxin proteins, a mechanism by which toxin-expressing bacteria protect themselves from DNA damage. Because toxin-induced DNA damage requires cell-cell interaction, we effectively demonstrated the presence of anti-toxin proteins on the surface of E. coli using flow cytometry in this experiment. The results indicated that the anti-toxin N terminal display is distinct from the C terminal display. Although it cannot be detected using flow cytometry, it can be recognized using a western blot. Additionally, coculturing with HeLa cells demonstrated that E. coli generating toxin can induce DNA double-strand breaks and that E. coli expressing the anti-toxin can mitigate the DNA damage induced by the toxin.
590 $a School code: 0160.
650 4 $a Bioengineering. $3 657580
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650 4 $a Molecular biology. $3 517296
653 $a Clbs
653 $a Colibactin
653 $a Colorectal cancer
653 $a E. coli
653 $a pks
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690 $a 0307
710 2 $a Northeastern University. $b Bioengineering. $3 3434656
773 0 $t Masters Abstracts International $g 83-12.
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793 $a English
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