語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
FindBook
Google Book
Amazon
博客來
Microfluidic Platforms towards Virus Detection and Cancer Diagnosis Based on Tumor Cells.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Microfluidic Platforms towards Virus Detection and Cancer Diagnosis Based on Tumor Cells./
作者:
Xia, Yiqiu.
出版者:
Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:
165 p.
附註:
Source: Dissertations Abstracts International, Volume: 83-12, Section: B.
Contained By:
Dissertations Abstracts International83-12B.
標題:
Biomedical engineering. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29267428
ISBN:
9798819377239
Microfluidic Platforms towards Virus Detection and Cancer Diagnosis Based on Tumor Cells.
Xia, Yiqiu.
Microfluidic Platforms towards Virus Detection and Cancer Diagnosis Based on Tumor Cells.
- Ann Arbor : ProQuest Dissertations & Theses, 2019 - 165 p.
Source: Dissertations Abstracts International, Volume: 83-12, Section: B.
Thesis (Ph.D.)--The Pennsylvania State University, 2019.
As a major healthcare concern, highly pathogenic viral infection can spread globally with modern transportation. Viral infectious diseases have caused some of the deadliest pandemics and heavily damaged global economy in recorded human history. As we prepare for the next major emerging viral infectious disease outbreak, there is an urgent need for the development of new techniques that can rapidly detect viruses and perform surveillance of viral infectious diseases at any location. On the other and, cancer is a major disease in human society nowadays, leading to the second most deaths worldwide. Circulating tumor cell (CTC) has been established as a liquid biopsy marker, however, there are demands of fast and accurate CTC detection. Microfluidics has the advantages of high throughput, high sensitivity, accurate flow rate control and low cost, allowing it well suited for virus and cancer diagnosis. Besides, the geometry of microfluidics allows precisely controlling of the physical, chemical, biological, and physiological environment at the cellular level or even at the molecular level for fundamental studies of cancers.My major works can be classified into two categories, microfluidic devices for virus diagnosis and microfluidic platforms towards cancer diagnosis. For the virus diagnosis, one microfluidic device for size-based virus isolation and another one for immunoaffinity-based virus detection are developed, respectively. In the first device, inter-wire size-tunable porous silicon nanowire forest is embedded inside the microfluidic channel to trap avian influenza viruses based on their size and then release trapped viruses by nanowire degradation. About 50% of virus can be captured and 60% of trapped virus can be released for culture and further analysis. In the second device, immunoassay is employed inside the channels to capture and detect virus in only ~1.5 hours. Colorimetric reaction with gold nanoparticles and silver enhancer allow detection with naked eyes with about one order of magnitude better than conventional fluorescent enzyme-linked immunosorbent assay (ELISA). Simply by introducing an optical detection scheme with a smartphone detection system, the sensitivity can be 30 times better than conventional fluorescent ELISA. Two microfluidic platforms were developed toward cancer diagnosis. The first microfluidic platform aims to study the process of CTC size-based microfiltration and cancer cell translocating through micro constrictions by mimicking the microfiltration process and in vivo micro-constrictions inside a microfluidic device. It is found that the deformability and size of nucleus instead of the whole cell dominate cellular translocation through micro constrictions under the normal physiological pressure range used by CTC microfiltration. The result is consistent with the size-based enrichment of white blood cells and CTCs from peripheral blood of metastatic cancer patients using a CTC microfilter previously developed in my group. It indicates that the size and deformability of cell nucleus play a critical role in CTC size-based microfiltration and potentially cancer cell translocating micro constrictions in vivo. The second microfluidic platform can measure the Young's modulus of cells in a high throughput fashion by applying a micropipette aspiration model in an array of micro constrictions. Using this device, a subtype of cancer cells with a softer mechanical phenotype can be enriched. This subtype of cancer cells shows enhanced invasive-related properties and can be used for further study of metastasis and cancer cell heterogeneity.
ISBN: 9798819377239Subjects--Topical Terms:
535387
Biomedical engineering.
Subjects--Index Terms:
Circulating tumor cell detectuib
Microfluidic Platforms towards Virus Detection and Cancer Diagnosis Based on Tumor Cells.
LDR
:04794nmm a2200373 4500
001
2348134
005
20220906075214.5
008
241004s2019 ||||||||||||||||| ||eng d
020
$a
9798819377239
035
$a
(MiAaPQ)AAI29267428
035
$a
AAI29267428
040
$a
MiAaPQ
$c
MiAaPQ
100
1
$a
Xia, Yiqiu.
$3
3687452
245
1 0
$a
Microfluidic Platforms towards Virus Detection and Cancer Diagnosis Based on Tumor Cells.
260
1
$a
Ann Arbor :
$b
ProQuest Dissertations & Theses,
$c
2019
300
$a
165 p.
500
$a
Source: Dissertations Abstracts International, Volume: 83-12, Section: B.
500
$a
Advisor: Zheng, Siyang.
502
$a
Thesis (Ph.D.)--The Pennsylvania State University, 2019.
520
$a
As a major healthcare concern, highly pathogenic viral infection can spread globally with modern transportation. Viral infectious diseases have caused some of the deadliest pandemics and heavily damaged global economy in recorded human history. As we prepare for the next major emerging viral infectious disease outbreak, there is an urgent need for the development of new techniques that can rapidly detect viruses and perform surveillance of viral infectious diseases at any location. On the other and, cancer is a major disease in human society nowadays, leading to the second most deaths worldwide. Circulating tumor cell (CTC) has been established as a liquid biopsy marker, however, there are demands of fast and accurate CTC detection. Microfluidics has the advantages of high throughput, high sensitivity, accurate flow rate control and low cost, allowing it well suited for virus and cancer diagnosis. Besides, the geometry of microfluidics allows precisely controlling of the physical, chemical, biological, and physiological environment at the cellular level or even at the molecular level for fundamental studies of cancers.My major works can be classified into two categories, microfluidic devices for virus diagnosis and microfluidic platforms towards cancer diagnosis. For the virus diagnosis, one microfluidic device for size-based virus isolation and another one for immunoaffinity-based virus detection are developed, respectively. In the first device, inter-wire size-tunable porous silicon nanowire forest is embedded inside the microfluidic channel to trap avian influenza viruses based on their size and then release trapped viruses by nanowire degradation. About 50% of virus can be captured and 60% of trapped virus can be released for culture and further analysis. In the second device, immunoassay is employed inside the channels to capture and detect virus in only ~1.5 hours. Colorimetric reaction with gold nanoparticles and silver enhancer allow detection with naked eyes with about one order of magnitude better than conventional fluorescent enzyme-linked immunosorbent assay (ELISA). Simply by introducing an optical detection scheme with a smartphone detection system, the sensitivity can be 30 times better than conventional fluorescent ELISA. Two microfluidic platforms were developed toward cancer diagnosis. The first microfluidic platform aims to study the process of CTC size-based microfiltration and cancer cell translocating through micro constrictions by mimicking the microfiltration process and in vivo micro-constrictions inside a microfluidic device. It is found that the deformability and size of nucleus instead of the whole cell dominate cellular translocation through micro constrictions under the normal physiological pressure range used by CTC microfiltration. The result is consistent with the size-based enrichment of white blood cells and CTCs from peripheral blood of metastatic cancer patients using a CTC microfilter previously developed in my group. It indicates that the size and deformability of cell nucleus play a critical role in CTC size-based microfiltration and potentially cancer cell translocating micro constrictions in vivo. The second microfluidic platform can measure the Young's modulus of cells in a high throughput fashion by applying a micropipette aspiration model in an array of micro constrictions. Using this device, a subtype of cancer cells with a softer mechanical phenotype can be enriched. This subtype of cancer cells shows enhanced invasive-related properties and can be used for further study of metastasis and cancer cell heterogeneity.
590
$a
School code: 0176.
650
4
$a
Biomedical engineering.
$3
535387
650
4
$a
Oncology.
$3
751006
650
4
$a
Health care management.
$3
2122906
650
4
$a
Virology.
$3
642304
653
$a
Circulating tumor cell detectuib
653
$a
Virus detection
653
$a
Cancer diagnosis
653
$a
Smartphone detection system
653
$a
Microfluidic platforms
690
$a
0720
690
$a
0541
690
$a
0992
690
$a
0769
710
2
$a
The Pennsylvania State University.
$3
699896
773
0
$t
Dissertations Abstracts International
$g
83-12B.
790
$a
0176
791
$a
Ph.D.
792
$a
2019
793
$a
English
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=29267428
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9470572
電子資源
11.線上閱覽_V
電子書
EB
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入