東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

3D Nerve Integrated Tissue on a Chip and Organoid Models for Exploring Mechanotransduction and Gene Expression.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	3D Nerve Integrated Tissue on a Chip and Organoid Models for Exploring Mechanotransduction and Gene Expression./
作者:	Bobo, Justin.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	115 p.
附註:	Source: Dissertations Abstracts International, Volume: 83-05, Section: B.
Contained By:	Dissertations Abstracts International83-05B.
標題:	Mechanical engineering. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28652118
ISBN:	9798492732882

3D Nerve Integrated Tissue on a Chip and Organoid Models for Exploring Mechanotransduction and Gene Expression.
Bobo, Justin.

3D Nerve Integrated Tissue on a Chip and Organoid Models for Exploring Mechanotransduction and Gene Expression. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 115 p.

Source: Dissertations Abstracts International, Volume: 83-05, Section: B.

Thesis (Ph.D.)--Carnegie Mellon University, 2021.

This item must not be sold to any third party vendors.

The ever-growing need to understand and engineer biological and biochemical mechanisms of cells and materials that aim to study, restore, maintain, and improve the treatment of disease has led to the emergence of in vitro models in the field of tissue engineering. The evolution of tissue-engineered models holds promise for mimicking the responses of biological functionality of physiological systems. Such tissue models involve the combination of cells, engineering, materials methods, and suitable biochemical and biophysical factors, allowing the microenvironmental signals to be influenced by their three-dimensional (3D) surroundings, including the extracellular matrix (ECM) scaffold architectures. This distinct, complex organization enables a versatile design and distinctive advantages over foundational 2D studies. 3D ECM systems and 3D ECM scaffold systems provide more complex cell-to-cell communication, realistic cell-ECM interaction, and spatial organization. Engineered tissue models, encompassing methods such as brain models with neurons, hold promise in providing more predictive, robust, and physiologically relevant platforms to find and study disease, inflammation, and injured states.Despite its potential to revolutionize diagnostics, therapeutics, treatment, and healing protocols, our present understanding of neural activity response to mechanical loading on neurons in vitro is mainly limited to local stimulation of single or multiple neurons cultured over planar substrates. To better simulate neuron response in 3D to mechanics, we developed a neuron-like nerve integrated tissue on a chip (NITC) system embedded within a collagen matrix that, once subjected to our mechanical excitation test-bed (MET) system, converts mechanical stimuli into bio-active cues shifting intracellular signals. To accomplish this, we designed the MET system incorporating a voice coil actuator (VCA) with custom printed 3D parts controlled by a power supply and function generator synchronized with live image capture.This work then studies high-throughput biology integration of the MET system to aid in rapid testing. Here we demonstrate an increase in the number of independent parameters that define the functional configuration and automating the input parameters that govern the applied external mechanical stimuli to NITC devices. Here, we observed a molecular signature, calcium, is expressed as a function of the applied strain rate.This work was further enhanced by building upon the reductionist organ-on-a-chip approach by using cerebral organoids with the MET system to validate neural identity and activity, explore the impact of neuroprotective strategies, and analyze the transcriptome of gene expression within RNA.We believe the on-chip approach and the cerebral organoid model combined with the MET system will have implications in various areas, including tissue on-chip systems, bio-materials, neuromechanics, cellular dynamics, gene expression, regeneration, and fundamental findings associating the genome with external mechanics.

ISBN: 9798492732882Subjects--Topical Terms:

649730
Mechanical engineering.
Subjects--Index Terms:

Calcium

3D Nerve Integrated Tissue on a Chip and Organoid Models for Exploring Mechanotransduction and Gene Expression.
LDR:04279nmm a2200385 4500 001 2347172
005 20220719070521.5
008 241004s2021 ||||||||||||||||| ||eng d
020 $a 9798492732882
035 $a (MiAaPQ)AAI28652118
035 $a AAI28652118
040 $a MiAaPQ $c MiAaPQ
100 1 $a Bobo, Justin. $0 (orcid)0000-0002-6519-7989 $3 3686387
245 1 0 $a 3D Nerve Integrated Tissue on a Chip and Organoid Models for Exploring Mechanotransduction and Gene Expression.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2021
300 $a 115 p.
500 $a Source: Dissertations Abstracts International, Volume: 83-05, Section: B.
500 $a Advisor: LeDuc, Philip R.
502 $a Thesis (Ph.D.)--Carnegie Mellon University, 2021.
506 $a This item must not be sold to any third party vendors.
520 $a The ever-growing need to understand and engineer biological and biochemical mechanisms of cells and materials that aim to study, restore, maintain, and improve the treatment of disease has led to the emergence of in vitro models in the field of tissue engineering. The evolution of tissue-engineered models holds promise for mimicking the responses of biological functionality of physiological systems. Such tissue models involve the combination of cells, engineering, materials methods, and suitable biochemical and biophysical factors, allowing the microenvironmental signals to be influenced by their three-dimensional (3D) surroundings, including the extracellular matrix (ECM) scaffold architectures. This distinct, complex organization enables a versatile design and distinctive advantages over foundational 2D studies. 3D ECM systems and 3D ECM scaffold systems provide more complex cell-to-cell communication, realistic cell-ECM interaction, and spatial organization. Engineered tissue models, encompassing methods such as brain models with neurons, hold promise in providing more predictive, robust, and physiologically relevant platforms to find and study disease, inflammation, and injured states.Despite its potential to revolutionize diagnostics, therapeutics, treatment, and healing protocols, our present understanding of neural activity response to mechanical loading on neurons in vitro is mainly limited to local stimulation of single or multiple neurons cultured over planar substrates. To better simulate neuron response in 3D to mechanics, we developed a neuron-like nerve integrated tissue on a chip (NITC) system embedded within a collagen matrix that, once subjected to our mechanical excitation test-bed (MET) system, converts mechanical stimuli into bio-active cues shifting intracellular signals. To accomplish this, we designed the MET system incorporating a voice coil actuator (VCA) with custom printed 3D parts controlled by a power supply and function generator synchronized with live image capture.This work then studies high-throughput biology integration of the MET system to aid in rapid testing. Here we demonstrate an increase in the number of independent parameters that define the functional configuration and automating the input parameters that govern the applied external mechanical stimuli to NITC devices. Here, we observed a molecular signature, calcium, is expressed as a function of the applied strain rate.This work was further enhanced by building upon the reductionist organ-on-a-chip approach by using cerebral organoids with the MET system to validate neural identity and activity, explore the impact of neuroprotective strategies, and analyze the transcriptome of gene expression within RNA.We believe the on-chip approach and the cerebral organoid model combined with the MET system will have implications in various areas, including tissue on-chip systems, bio-materials, neuromechanics, cellular dynamics, gene expression, regeneration, and fundamental findings associating the genome with external mechanics.
590 $a School code: 0041.
650 4 $a Mechanical engineering. $3 649730
650 4 $a Biomechanics. $3 548685
650 4 $a Neurosciences. $3 588700
653 $a Calcium
653 $a Gene expression
653 $a Mechanostimulation
653 $a Micro-scale
653 $a Organoids
653 $a Tissue-on-chip
690 $a 0548
690 $a 0648
690 $a 0317
710 2 $a Carnegie Mellon University. $b Mechanical Engineering. $3 2096240
773 0 $t Dissertations Abstracts International $g 83-05B.
790 $a 0041
791 $a Ph.D.
792 $a 2021
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28652118