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DNA-Based Nanosensors for Small Molecules and Proteins.

Record Type:	Electronic resources : Monograph/item
Title/Author:	DNA-Based Nanosensors for Small Molecules and Proteins./
Author:	Morales, Jennifer Monique.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	213 p.
Notes:	Source: Dissertations Abstracts International, Volume: 80-04, Section: B.
Contained By:	Dissertations Abstracts International80-04B.
Subject:	Bioengineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10934065
ISBN:	9780438385436

DNA-Based Nanosensors for Small Molecules and Proteins.
Morales, Jennifer Monique.

DNA-Based Nanosensors for Small Molecules and Proteins. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 213 p.

Source: Dissertations Abstracts International, Volume: 80-04, Section: B.

Thesis (Ph.D.)--Northeastern University, 2018.

This item is not available from ProQuest Dissertations & Theses.

Biomolecules play an important role in homeostasis regulation of the body and disease progression. Continuous monitoring of these molecules can provide an understanding of their physiological roles and elucidate the overlap between research and medicine. Sensors for continuously monitoring in vivo require a combination of traits not seen in sample-based sensors including small size, reversibility and spatiotemporal resolution in tissue. The concepts conveyed within this dissertation outline the concept, design and characterization of two DNA nanosensor platforms: 1) a fluorescent DNA-dendrimer platform for sensing small molecules and 2) a photoacoustic DNA nanomachine sensor platform for proteins. We chose DNA as the structural backbone for our platforms because it is a well-known building construct, highly modifiable and self-assembles into precise nanostructures. First, we developed a fluorescent nanosensor for neurotransmitters as a target for small molecules utilizing a dendrimer scaffold that organizes a hierarchy of sensing components to maximize signal and increase sensitivity. To measure acetylcholine release we improved on the sensors structure, size and organization of sensing components over three generations. Second, we developed a photoacoustic nanosensor for cytokines using a two-step binding complex to drive a DNA nanomachine. This proof-of-concept sensor utilizes a unique reporting mechanism to increase photoacoustic signal via phthalocyanine dye stacking. Together, these sensors improve on current technology by providing new tools to explore biological questions with temporal and spatial resolution. These platforms are easily tailored to tune sensitivity, response and dynamic range for different biomolecules by switching out sensing components. This adaptability results in adaptable sensors for different sensing mechanisms and targets of interest.

ISBN: 9780438385436Subjects--Topical Terms:

657580
Bioengineering.
Subjects--Index Terms:

DNA

DNA-Based Nanosensors for Small Molecules and Proteins.
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300 $a 213 p.
500 $a Source: Dissertations Abstracts International, Volume: 80-04, Section: B.
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500 $a Advisor: Clark, Heather A.
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506 $a This item is not available from ProQuest Dissertations & Theses.
506 $a This item must not be sold to any third party vendors.
520 $a Biomolecules play an important role in homeostasis regulation of the body and disease progression. Continuous monitoring of these molecules can provide an understanding of their physiological roles and elucidate the overlap between research and medicine. Sensors for continuously monitoring in vivo require a combination of traits not seen in sample-based sensors including small size, reversibility and spatiotemporal resolution in tissue. The concepts conveyed within this dissertation outline the concept, design and characterization of two DNA nanosensor platforms: 1) a fluorescent DNA-dendrimer platform for sensing small molecules and 2) a photoacoustic DNA nanomachine sensor platform for proteins. We chose DNA as the structural backbone for our platforms because it is a well-known building construct, highly modifiable and self-assembles into precise nanostructures. First, we developed a fluorescent nanosensor for neurotransmitters as a target for small molecules utilizing a dendrimer scaffold that organizes a hierarchy of sensing components to maximize signal and increase sensitivity. To measure acetylcholine release we improved on the sensors structure, size and organization of sensing components over three generations. Second, we developed a photoacoustic nanosensor for cytokines using a two-step binding complex to drive a DNA nanomachine. This proof-of-concept sensor utilizes a unique reporting mechanism to increase photoacoustic signal via phthalocyanine dye stacking. Together, these sensors improve on current technology by providing new tools to explore biological questions with temporal and spatial resolution. These platforms are easily tailored to tune sensitivity, response and dynamic range for different biomolecules by switching out sensing components. This adaptability results in adaptable sensors for different sensing mechanisms and targets of interest.
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