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Dextran hydrogel preparation and applications in biomedical engineering.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Dextran hydrogel preparation and applications in biomedical engineering./
Author:	Zhang, Rongsheng.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2004,
Description:	265 p.
Notes:	Source: Dissertations Abstracts International, Volume: 70-12, Section: C.
Contained By:	Dissertations Abstracts International70-12C.
Subject:	Biophysics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=U177041
ISBN:	9781073291953

Dextran hydrogel preparation and applications in biomedical engineering.
Zhang, Rongsheng.

Dextran hydrogel preparation and applications in biomedical engineering. - Ann Arbor : ProQuest Dissertations & Theses, 2004 - 265 p.

Source: Dissertations Abstracts International, Volume: 70-12, Section: C.

Thesis (Ph.D.)--University of Bath (United Kingdom), 2004.

This item is not available from ProQuest Dissertations & Theses.

This project investigates dextran hydrogel fabrication for various practical applications in biomedical engineering, based on a number of cross-linking reactions. D-glucose sensitive dextran/Con-A hydrogel membranes can be synthesized via a nucleophilic replacement reaction in a triazine ring. The resultant hydrogels are capable of releasing insulin in a dose-dependent response to changes in D-glucose concentrations. An alternative procedure for the synthesis of D-glucose responsive hydrogels based on carbodiimide chemistry allows production of gels with varying and controllable levels of protein and carboxylic group substitution. Final a CM-dextran with 5 COOH/CM-dextran (10-4 Mol/g) and a proportion of cross-linking agents at 7 EDC/NHS (W/W), a weight ratio of 0.10 CM-dextran/hydrogel (W/W) was the optimum for forming cross-linkages. pH-sensitive hydrogels were also formed by the intermolecular cross-linking of CM-dextran dextran with a high level of carboxylic group substitution using EDC and NHS. Preliminary studies with primary human dermal fibroblast cultures showed that these gels are capable of supporting cell growth. A proof of concept study was conducted to assess the potential of these gels to act as sensing elements by cast a diffraction grating into the gel surface. Test experiments showed the size of diffraction and reflection spots from an incident laser beam changed in size as the gel changed dimensions in response to changes in environment. A further application of affinity material was the fabrication of membranes containing protein and CB. Results proved that intrinsic competitive binding existed and effectively shield the ligand from extern weak effects interactions. The D-glucose sensitive hydrogel was also fabricated into bead. Size exclusion chromatography (SEC) was employed to detect the effect of D-glucose on throughout of protein across these beads. This serves both to confirm the effect of glucose on pore size and offer the possibility of a novel adaptive size exclusive resin.

ISBN: 9781073291953Subjects--Topical Terms:

518360
Biophysics.

Dextran hydrogel preparation and applications in biomedical engineering.
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020 $a 9781073291953
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100 1 $a Zhang, Rongsheng. $3 3686220
245 1 0 $a Dextran hydrogel preparation and applications in biomedical engineering.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2004
300 $a 265 p.
500 $a Source: Dissertations Abstracts International, Volume: 70-12, Section: C.
500 $a Publisher info.: Dissertation/Thesis.
502 $a Thesis (Ph.D.)--University of Bath (United Kingdom), 2004.
506 $a This item is not available from ProQuest Dissertations & Theses.
520 $a This project investigates dextran hydrogel fabrication for various practical applications in biomedical engineering, based on a number of cross-linking reactions. D-glucose sensitive dextran/Con-A hydrogel membranes can be synthesized via a nucleophilic replacement reaction in a triazine ring. The resultant hydrogels are capable of releasing insulin in a dose-dependent response to changes in D-glucose concentrations. An alternative procedure for the synthesis of D-glucose responsive hydrogels based on carbodiimide chemistry allows production of gels with varying and controllable levels of protein and carboxylic group substitution. Final a CM-dextran with 5 COOH/CM-dextran (10-4 Mol/g) and a proportion of cross-linking agents at 7 EDC/NHS (W/W), a weight ratio of 0.10 CM-dextran/hydrogel (W/W) was the optimum for forming cross-linkages. pH-sensitive hydrogels were also formed by the intermolecular cross-linking of CM-dextran dextran with a high level of carboxylic group substitution using EDC and NHS. Preliminary studies with primary human dermal fibroblast cultures showed that these gels are capable of supporting cell growth. A proof of concept study was conducted to assess the potential of these gels to act as sensing elements by cast a diffraction grating into the gel surface. Test experiments showed the size of diffraction and reflection spots from an incident laser beam changed in size as the gel changed dimensions in response to changes in environment. A further application of affinity material was the fabrication of membranes containing protein and CB. Results proved that intrinsic competitive binding existed and effectively shield the ligand from extern weak effects interactions. The D-glucose sensitive hydrogel was also fabricated into bead. Size exclusion chromatography (SEC) was employed to detect the effect of D-glucose on throughout of protein across these beads. This serves both to confirm the effect of glucose on pore size and offer the possibility of a novel adaptive size exclusive resin.
590 $a School code: 0690.
650 4 $a Biophysics. $3 518360
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