東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

Studies of the host range restriction, antigenic type and hemagglutination properties of canine and feline parvoviruses.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Studies of the host range restriction, antigenic type and hemagglutination properties of canine and feline parvoviruses./
作者:	Chang, Shwu-Fen.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 1993,
面頁冊數:	159 p.
附註:	Source: Dissertations Abstracts International, Volume: 55-08, Section: B.
Contained By:	Dissertations Abstracts International55-08B.
標題:	Veterinary services. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9408370
ISBN:	9798535528632

Studies of the host range restriction, antigenic type and hemagglutination properties of canine and feline parvoviruses.
Chang, Shwu-Fen.

Studies of the host range restriction, antigenic type and hemagglutination properties of canine and feline parvoviruses. - Ann Arbor : ProQuest Dissertations & Theses, 1993 - 159 p.

Source: Dissertations Abstracts International, Volume: 55-08, Section: B.

Thesis (Ph.D.)--Cornell University, 1993.

This item must not be added to any third party search indexes.

Canine parovirus (CPV) and feline panleukopenia virus (FPV) are classified as host range variants of the feline parvovirus among the genus Parvovirus. In spite of the high similarity in DNA sequence and genome organization between CPV and FPV, the viruses can be readily distinguished by their different in vivo and in vitro host ranges, distinct antigenic types and pH-dependent hemagglutination (HA) phenotypes. Previous studies have indicated that sequences which determine the viral host range, antigenic type and pH-dependence of HA map into the viral capsid protein gene (Parrish, et al., 1988a; Parrish, 1991). In order to dissect the role of virus strain-specific nucleotides within that region in the determination of the biological properties of the viruses, genetic mutagenesis and recombinations were used to prepare a series of recombinant viruses for biological analysis. The results suggest that several residues affecting the interactions between the VP2 molecules of the viral capsid determine the viral in vitro host range. As few as two CPV-specific residues (Asn-93, and Asn-323) were able to extend the in vitro host range of viruses with an FPV background to canine cells. Viruses with a CPV genetic background which had changes at any one of those residues as well as Ala-103 lost the canine host range in vitro. Asn-93 alone was responsible for the epitope recognized by CPV-specific MAbs. Asn-323 and Asn-375 coordinately determined the pH-dependence of HA of CPV and FPV. FPV appeared to be transported to the same intracellular location in both permissive and nonpermissive cells, and there was no significant difference in the transport and cellular localization of CPV and FPV capsids within canine A72 cells. At 16 hours after inoculation, both viruses were found to be localized at the perinuclear region of both permissive and nonpermissive cells. The canine host range restriction of FPV infection was shown to be overcome by directly introducing the viral infectious plasmid DNA into the A72 cells. These results indicated that the restriction of FPV growth in canine A72 cells was blocked after virus binding and internalization into the cells, but before the amplification of the viral DNA in the nucleus.

ISBN: 9798535528632Subjects--Topical Terms:

3433982
Veterinary services.

Studies of the host range restriction, antigenic type and hemagglutination properties of canine and feline parvoviruses.
LDR:03334nmm a2200301 4500 001 2344747
005 20220531065747.5
008 241004s1993 ||||||||||||||||| ||eng d
020 $a 9798535528632
035 $a (MiAaPQ)AAI9408370
035 $a AAI9408370
040 $a MiAaPQ $c MiAaPQ
100 1 $a Chang, Shwu-Fen. $3 3683552
245 1 0 $a Studies of the host range restriction, antigenic type and hemagglutination properties of canine and feline parvoviruses.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 1993
300 $a 159 p.
500 $a Source: Dissertations Abstracts International, Volume: 55-08, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
502 $a Thesis (Ph.D.)--Cornell University, 1993.
506 $a This item must not be added to any third party search indexes.
506 $a This item must not be sold to any third party vendors.
520 $a Canine parovirus (CPV) and feline panleukopenia virus (FPV) are classified as host range variants of the feline parvovirus among the genus Parvovirus. In spite of the high similarity in DNA sequence and genome organization between CPV and FPV, the viruses can be readily distinguished by their different in vivo and in vitro host ranges, distinct antigenic types and pH-dependent hemagglutination (HA) phenotypes. Previous studies have indicated that sequences which determine the viral host range, antigenic type and pH-dependence of HA map into the viral capsid protein gene (Parrish, et al., 1988a; Parrish, 1991). In order to dissect the role of virus strain-specific nucleotides within that region in the determination of the biological properties of the viruses, genetic mutagenesis and recombinations were used to prepare a series of recombinant viruses for biological analysis. The results suggest that several residues affecting the interactions between the VP2 molecules of the viral capsid determine the viral in vitro host range. As few as two CPV-specific residues (Asn-93, and Asn-323) were able to extend the in vitro host range of viruses with an FPV background to canine cells. Viruses with a CPV genetic background which had changes at any one of those residues as well as Ala-103 lost the canine host range in vitro. Asn-93 alone was responsible for the epitope recognized by CPV-specific MAbs. Asn-323 and Asn-375 coordinately determined the pH-dependence of HA of CPV and FPV. FPV appeared to be transported to the same intracellular location in both permissive and nonpermissive cells, and there was no significant difference in the transport and cellular localization of CPV and FPV capsids within canine A72 cells. At 16 hours after inoculation, both viruses were found to be localized at the perinuclear region of both permissive and nonpermissive cells. The canine host range restriction of FPV infection was shown to be overcome by directly introducing the viral infectious plasmid DNA into the A72 cells. These results indicated that the restriction of FPV growth in canine A72 cells was blocked after virus binding and internalization into the cells, but before the amplification of the viral DNA in the nucleus.
590 $a School code: 0058.
650 4 $a Veterinary services. $3 3433982
690 $a 0778
710 2 $a Cornell University. $3 530586
773 0 $t Dissertations Abstracts International $g 55-08B.
790 $a 0058
791 $a Ph.D.
792 $a 1993
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9408370