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The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep./
Author:	Middleton, Denzel Deshawn.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	94 p.
Notes:	Source: Dissertations Abstracts International, Volume: 83-05, Section: B.
Contained By:	Dissertations Abstracts International83-05B.
Subject:	Animal sciences. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28811942
ISBN:	9798494452191

The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep.
Middleton, Denzel Deshawn.

The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 94 p.

Source: Dissertations Abstracts International, Volume: 83-05, Section: B.

Thesis (Ph.D.)--West Virginia University, 2021.

This item must not be sold to any third party vendors.

Little is known about differences observed in early immunological signaling events that result in cellular infiltration and Th2 cytokine production that mediate parasite expulsion. Parasite resistant St. Croix (STC) sheep have been used as model for full host protective immunity as they present the ability to respond to larval stages of H. contortus. RNA sequencing analysis of peripheral blood mononuclear cells (PBMC) stimulated with H. contortus larval antigen (HcLA) revealed significant upregulation in NLRP3 and its pathway component TLR4. Predicted protein structural software identified distinct differences in NLRP3 protein structure in STC and parasite susceptible Suffolk (SUF) sheep. NLRP3 has been widely studied in a classic model of inflammatory infection, but little is known about its role in helminth infections. The inhibition of NLRP3 in a classic model of infection reduced STC PBMC responses on both the transcript and protein level but had no effect on the transcript level for SUF PBMC only the protein level. In an HcLA infection model PBMC from SUF with the inhibition of NLRP3 resulted in the upregulation of TLR4, TLR2, and IL-10. We hypothesize NLRP3 loss of function to have a significant impact on components of TLR4 signaling and inflammatory immune activation to pathogenic challenge. The inhibition of a dysfunctional NLRP3 receptor in SUF sheep maybe a potential target for chemotherapeutics to achieve full host protective immunity.

ISBN: 9798494452191Subjects--Topical Terms:

3174829
Animal sciences.
Subjects--Index Terms:

Haemonchus contortus

The Effects of Genetic Differences in an Innate Immune Receptor on Immune Responses to Haemonchus contortus in Sheep.
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506 $a This item must not be sold to any third party vendors.
520 $a Little is known about differences observed in early immunological signaling events that result in cellular infiltration and Th2 cytokine production that mediate parasite expulsion. Parasite resistant St. Croix (STC) sheep have been used as model for full host protective immunity as they present the ability to respond to larval stages of H. contortus. RNA sequencing analysis of peripheral blood mononuclear cells (PBMC) stimulated with H. contortus larval antigen (HcLA) revealed significant upregulation in NLRP3 and its pathway component TLR4. Predicted protein structural software identified distinct differences in NLRP3 protein structure in STC and parasite susceptible Suffolk (SUF) sheep. NLRP3 has been widely studied in a classic model of inflammatory infection, but little is known about its role in helminth infections. The inhibition of NLRP3 in a classic model of infection reduced STC PBMC responses on both the transcript and protein level but had no effect on the transcript level for SUF PBMC only the protein level. In an HcLA infection model PBMC from SUF with the inhibition of NLRP3 resulted in the upregulation of TLR4, TLR2, and IL-10. We hypothesize NLRP3 loss of function to have a significant impact on components of TLR4 signaling and inflammatory immune activation to pathogenic challenge. The inhibition of a dysfunctional NLRP3 receptor in SUF sheep maybe a potential target for chemotherapeutics to achieve full host protective immunity.
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