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Assembled Microgel Scaffolds for Dir...

Caldwell, Alexander Scott.

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Assembled Microgel Scaffolds for Directing Cell Fate and Function.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Assembled Microgel Scaffolds for Directing Cell Fate and Function./
作者:	Caldwell, Alexander Scott.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
面頁冊數:	216 p.
附註:	Source: Dissertations Abstracts International, Volume: 82-04, Section: B.
Contained By:	Dissertations Abstracts International82-04B.
標題:	Bioengineering. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28031098
ISBN:	9798672134680

Assembled Microgel Scaffolds for Directing Cell Fate and Function.
Caldwell, Alexander Scott.

Assembled Microgel Scaffolds for Directing Cell Fate and Function. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 216 p.

Source: Dissertations Abstracts International, Volume: 82-04, Section: B.

Thesis (Ph.D.)--University of Colorado at Boulder, 2020.

This item is not available from ProQuest Dissertations & Theses.

Synthetic hydrogels provide a versatile platform for the design of cell culture platforms that can impart specific cues to cells. These materials have wide reaching applications in vitro, as they can recreate the complex biochemical and biomechanical signals that exist in the cellular microenvironment. Furthermore, hydrogels can function as a delivery vehicle in cell-based therapies, where bioactive cues can be included to support cell viability and direct cellular function, improving therapy efficacy. The design of networks that can present multiple stimuli to direct cellular behavior in several, independent manners are especially attractive.To add to this growing body of research, this thesis aims to develop cell culture platforms that can control cell-cell and cell-matrix interactions in order to direct cellular behavior. Specifically, an assembled porous hydrogel scaffold is designed utilizing co-reactive bio-click microgels. The cytocompatible nature of this platform, as well as how microgel size can be exploited to alter scaffold porosity and cell morphology, is first demonstrated. This study provides insight into how scaffold structure can be used to direct cell behavior. Following this, scaffold porosity is investigated over a broader range to control cell clustering and secretory properties. Taking inspiration from controlling cell-cell interactions, a cellular mimetic peptide could be included within the scaffolds to further control cytokine secretion. Finally, we set out to further explore how bioactive cues could be included within hydrogel scaffolds to direct cell behavior. In the first iteration of this, bio-click reactions were exploited to control the presentation of multiple proteins simultaneously to control fibroblast activation and calcification. Next, microgel scaffolds were designed with a tethered anti-inflammatory protein to mimic the immunomodulatory capabilities of hMSCs. Collectively, this work demonstrates how biomaterial design can be leveraged to direct cellular function in numerous ways in the context of regenerative medicine and cell-based therapies.

ISBN: 9798672134680Subjects--Topical Terms:

657580
Bioengineering.
Subjects--Index Terms:

Bio-click

Assembled Microgel Scaffolds for Directing Cell Fate and Function.
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500 $a Source: Dissertations Abstracts International, Volume: 82-04, Section: B.
500 $a Advisor: Anseth, Kristi.
502 $a Thesis (Ph.D.)--University of Colorado at Boulder, 2020.
506 $a This item is not available from ProQuest Dissertations & Theses.
506 $a This item must not be sold to any third party vendors.
520 $a Synthetic hydrogels provide a versatile platform for the design of cell culture platforms that can impart specific cues to cells. These materials have wide reaching applications in vitro, as they can recreate the complex biochemical and biomechanical signals that exist in the cellular microenvironment. Furthermore, hydrogels can function as a delivery vehicle in cell-based therapies, where bioactive cues can be included to support cell viability and direct cellular function, improving therapy efficacy. The design of networks that can present multiple stimuli to direct cellular behavior in several, independent manners are especially attractive.To add to this growing body of research, this thesis aims to develop cell culture platforms that can control cell-cell and cell-matrix interactions in order to direct cellular behavior. Specifically, an assembled porous hydrogel scaffold is designed utilizing co-reactive bio-click microgels. The cytocompatible nature of this platform, as well as how microgel size can be exploited to alter scaffold porosity and cell morphology, is first demonstrated. This study provides insight into how scaffold structure can be used to direct cell behavior. Following this, scaffold porosity is investigated over a broader range to control cell clustering and secretory properties. Taking inspiration from controlling cell-cell interactions, a cellular mimetic peptide could be included within the scaffolds to further control cytokine secretion. Finally, we set out to further explore how bioactive cues could be included within hydrogel scaffolds to direct cell behavior. In the first iteration of this, bio-click reactions were exploited to control the presentation of multiple proteins simultaneously to control fibroblast activation and calcification. Next, microgel scaffolds were designed with a tethered anti-inflammatory protein to mimic the immunomodulatory capabilities of hMSCs. Collectively, this work demonstrates how biomaterial design can be leveraged to direct cellular function in numerous ways in the context of regenerative medicine and cell-based therapies.
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