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Determination of Anaerobic Endospore...

Aithinne, Kathleen A. N.

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Determination of Anaerobic Endospore-Forming Bacteria Cultivability Losses and Evaluation of Novel Media Collection Efficiencies Using Impingers.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Determination of Anaerobic Endospore-Forming Bacteria Cultivability Losses and Evaluation of Novel Media Collection Efficiencies Using Impingers./
Author:	Aithinne, Kathleen A. N.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	172 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-11, Section: B.
Contained By:	Dissertations Abstracts International81-11B.
Subject:	Environmental health. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27832488
ISBN:	9798644900633

Determination of Anaerobic Endospore-Forming Bacteria Cultivability Losses and Evaluation of Novel Media Collection Efficiencies Using Impingers.
Aithinne, Kathleen A. N.

Determination of Anaerobic Endospore-Forming Bacteria Cultivability Losses and Evaluation of Novel Media Collection Efficiencies Using Impingers. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 172 p.

Source: Dissertations Abstracts International, Volume: 81-11, Section: B.

Thesis (Ph.D.)--The University of Oklahoma Health Sciences Center, 2020.

This item must not be sold to any third party vendors.

Clostridioides difficile endospores (spores) are the causative agent of C. difficile infection, which is the third leading cause of healthcare-associate illness in the United States. Studies on aerosol concentrations and airborne dissemination of C. difficile spores throughout the healthcare environment have frequently resulted in negative air sampling results, even in lab-based studies where the aerosol was known to be present. This study evaluated impingement air sampling methods in the retention and collection of anaerobic endospore-forming bacteria and aimed to: (1) assess collection media and culture media differences in cultivability loss of C. difficile during low-volume air sampling using the SKC BioSampler™; (2) quantitatively assess the collection efficiency, relative to MCE filters, of liquid impingement media using novel and historical collection media when sampling aerosolized spores of C. difficile with the SKC BioSampler™; and (3) evaluate C. sporogenes, a commonly used surrogate for Clostridia species, for cultivability losses and establish whether novel impingement media will improve cultivability loss if seen. In the aim 1 dynamic retention trials, the BioSampler impingers retained cultivable C. difficile spores in the two novel media but not the two historically used media, and the chromogenic agar performed as well as the gold standard agar for the novel media. In aim 2, the cultivability losses were seen in the historically used media but not the novel media, and collected aerosol concentrations in novel media were on average two orders of magnitude greater than the aerosol concentrations of the historically used media, and novel media performed as well as or better than filtration cassettes for collection of viable C. difficile spores. In aim 3, C. sporogenes performed equally well with both novel and historical media, and did not exhibit the same cultivability losses and lack of retention as those seen during the aim 1 retention trials, indicating that C. sporogenes is not an adequate surrogate for C. difficile when collecting aerosol via impingement. This study demonstrated that impingement methods could be improved to reduce cultivability losses of C. difficile, and that the use of surrogates for air sampling using liquid impingement should be rigorously evaluated.

ISBN: 9798644900633Subjects--Topical Terms:

543032
Environmental health.
Subjects--Index Terms:

Aerosol

Determination of Anaerobic Endospore-Forming Bacteria Cultivability Losses and Evaluation of Novel Media Collection Efficiencies Using Impingers.
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500 $a Source: Dissertations Abstracts International, Volume: 81-11, Section: B.
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506 $a This item must not be sold to any third party vendors.
520 $a Clostridioides difficile endospores (spores) are the causative agent of C. difficile infection, which is the third leading cause of healthcare-associate illness in the United States. Studies on aerosol concentrations and airborne dissemination of C. difficile spores throughout the healthcare environment have frequently resulted in negative air sampling results, even in lab-based studies where the aerosol was known to be present. This study evaluated impingement air sampling methods in the retention and collection of anaerobic endospore-forming bacteria and aimed to: (1) assess collection media and culture media differences in cultivability loss of C. difficile during low-volume air sampling using the SKC BioSampler™; (2) quantitatively assess the collection efficiency, relative to MCE filters, of liquid impingement media using novel and historical collection media when sampling aerosolized spores of C. difficile with the SKC BioSampler™; and (3) evaluate C. sporogenes, a commonly used surrogate for Clostridia species, for cultivability losses and establish whether novel impingement media will improve cultivability loss if seen. In the aim 1 dynamic retention trials, the BioSampler impingers retained cultivable C. difficile spores in the two novel media but not the two historically used media, and the chromogenic agar performed as well as the gold standard agar for the novel media. In aim 2, the cultivability losses were seen in the historically used media but not the novel media, and collected aerosol concentrations in novel media were on average two orders of magnitude greater than the aerosol concentrations of the historically used media, and novel media performed as well as or better than filtration cassettes for collection of viable C. difficile spores. In aim 3, C. sporogenes performed equally well with both novel and historical media, and did not exhibit the same cultivability losses and lack of retention as those seen during the aim 1 retention trials, indicating that C. sporogenes is not an adequate surrogate for C. difficile when collecting aerosol via impingement. This study demonstrated that impingement methods could be improved to reduce cultivability losses of C. difficile, and that the use of surrogates for air sampling using liquid impingement should be rigorously evaluated.
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653 $a Impingers
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