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The Detection and Identification of ...

Thomas, Jalissa M.

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The Detection and Identification of Blood on Interfering Substrates Using Raman Spectroscopy and Hamand for Forensic Purposes.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The Detection and Identification of Blood on Interfering Substrates Using Raman Spectroscopy and Hamand for Forensic Purposes./
作者:	Thomas, Jalissa M.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	30 p.
附註:	Source: Masters Abstracts International, Volume: 82-11.
Contained By:	Masters Abstracts International82-11.
標題:	Analytical chemistry. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28492497
ISBN:	9798728242895

The Detection and Identification of Blood on Interfering Substrates Using Raman Spectroscopy and Hamand for Forensic Purposes.
Thomas, Jalissa M.

The Detection and Identification of Blood on Interfering Substrates Using Raman Spectroscopy and Hamand for Forensic Purposes. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 30 p.

Source: Masters Abstracts International, Volume: 82-11.

Thesis (M.S.)--State University of New York at Albany, 2021.

This item must not be sold to any third party vendors.

Body fluids, such as blood and semen, can provide invaluable information to a criminal investigation. As such, it is imperative that body fluid evidence is properly detected and identified. Our laboratory has developed a methodology to universally detect and identify body fluids using Raman spectroscopy and chemometrics. This methodology was developed under ideal conditions, which are rare in the field. Body fluid evidence can be found on substrates that produce a large Raman signal that can hinder detection of body fluid traces, which produce a weak signal. This project proposes the use of a statistical program called HAMAND (Hypothetical Addition Multivariate Analysis with Numerical Differentiation) as a solution to this problem. The software uses a reference spectrum of the target analyte to search for its contribution to the spectrum of an unknown sample. This proof-of-concept study utilized HAMAND to detect bloodstains on denim, a common substrate that produces a large interfering Raman signal. With HAMAND, we were able to successfully detect small traces of blood on denim. Subsequent preliminary tests were run to determine the applicability of the program to detect and differentiate bloodstains of different ages and distinguish between blood and other body fluids and potential false positives. The results indicate that HAMAND is not currently capable of performing these tasks. This methodology appears to have similar levels of accuracy to current biochemical tests for body fluid identification, but still has the distinct advantage of being nondestructive and rapid.

ISBN: 9798728242895Subjects--Topical Terms:

3168300
Analytical chemistry.
Subjects--Index Terms:

Body fluids

The Detection and Identification of Blood on Interfering Substrates Using Raman Spectroscopy and Hamand for Forensic Purposes.
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300 $a 30 p.
500 $a Source: Masters Abstracts International, Volume: 82-11.
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506 $a This item must not be sold to any third party vendors.
520 $a Body fluids, such as blood and semen, can provide invaluable information to a criminal investigation. As such, it is imperative that body fluid evidence is properly detected and identified. Our laboratory has developed a methodology to universally detect and identify body fluids using Raman spectroscopy and chemometrics. This methodology was developed under ideal conditions, which are rare in the field. Body fluid evidence can be found on substrates that produce a large Raman signal that can hinder detection of body fluid traces, which produce a weak signal. This project proposes the use of a statistical program called HAMAND (Hypothetical Addition Multivariate Analysis with Numerical Differentiation) as a solution to this problem. The software uses a reference spectrum of the target analyte to search for its contribution to the spectrum of an unknown sample. This proof-of-concept study utilized HAMAND to detect bloodstains on denim, a common substrate that produces a large interfering Raman signal. With HAMAND, we were able to successfully detect small traces of blood on denim. Subsequent preliminary tests were run to determine the applicability of the program to detect and differentiate bloodstains of different ages and distinguish between blood and other body fluids and potential false positives. The results indicate that HAMAND is not currently capable of performing these tasks. This methodology appears to have similar levels of accuracy to current biochemical tests for body fluid identification, but still has the distinct advantage of being nondestructive and rapid.
590 $a School code: 0668.
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653 $a Body fluids
653 $a Chemometrics
653 $a False positives
653 $a Raman spectroscopy
653 $a Substrate interference
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710 2 $a State University of New York at Albany. $b Chemistry. $3 1064619
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