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Selective Enrichment of Rare Mutatio...

Desai, Parth H.

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Selective Enrichment of Rare Mutations as a New Biotechnology to Study Dna Mismatch Repair Processes in Bacteria.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Selective Enrichment of Rare Mutations as a New Biotechnology to Study Dna Mismatch Repair Processes in Bacteria./
Author:	Desai, Parth H.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	126 p.
Notes:	Source: Masters Abstracts International, Volume: 82-01.
Contained By:	Masters Abstracts International82-01.
Subject:	Nanoscience. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27833175
ISBN:	9798662443983

Selective Enrichment of Rare Mutations as a New Biotechnology to Study Dna Mismatch Repair Processes in Bacteria.
Desai, Parth H.

Selective Enrichment of Rare Mutations as a New Biotechnology to Study Dna Mismatch Repair Processes in Bacteria. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 126 p.

Source: Masters Abstracts International, Volume: 82-01.

Thesis (M.S.)--The University of North Carolina at Greensboro, 2020.

This item is not available from ProQuest Dissertations & Theses.

DNA mismatch repair (MMR) is the key process which ensures the incorporation of correct nucleotides during DNA replication by recognizing and removing of incorrectly paired nucleotides from DNA. DNA replication can introduce a mismatched nucleotide at a rate of 10-5 to 10-6 nts/ replication cycle. If this mismatch is not corrected, then it becomes a permanent mutation after the next round of replication. Understanding the MMR mechanism can yield important insights into many aspects of human health, like the emergence of cancer and drug resistance in bacteria. To overcome experimental challenges with studying this process in living cells, we have developed a new method to enrich the rare genomic mutations by genotypic selection in a way that allows us to study the mismatch repair process in Escherichia coli, a model organism for MMR. We have shown the maximum 705,000-fold enrichment of DNA with a mutation even after a 10-6 times dilution by DNA with the wild-type sequence in vitro. After further optimization, we could then use this technique to directly measure MMR activity occurring in living E. coli. We expect this technique will open up new opportunities and research directions to study MMR-like processes in E. coli as well as different organisms, including Actinobacteria.

ISBN: 9798662443983Subjects--Topical Terms:

587832
Nanoscience.
Subjects--Index Terms:

Bacteria

Selective Enrichment of Rare Mutations as a New Biotechnology to Study Dna Mismatch Repair Processes in Bacteria.
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100 1 $a Desai, Parth H. $0 (orcid)0000-0002-5701-8370 $3 3562494
245 1 0 $a Selective Enrichment of Rare Mutations as a New Biotechnology to Study Dna Mismatch Repair Processes in Bacteria.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 126 p.
500 $a Source: Masters Abstracts International, Volume: 82-01.
500 $a Includes supplementary digital materials.
500 $a Advisor: Josephs, Eric A.
502 $a Thesis (M.S.)--The University of North Carolina at Greensboro, 2020.
506 $a This item is not available from ProQuest Dissertations & Theses.
506 $a This item must not be sold to any third party vendors.
520 $a DNA mismatch repair (MMR) is the key process which ensures the incorporation of correct nucleotides during DNA replication by recognizing and removing of incorrectly paired nucleotides from DNA. DNA replication can introduce a mismatched nucleotide at a rate of 10-5 to 10-6 nts/ replication cycle. If this mismatch is not corrected, then it becomes a permanent mutation after the next round of replication. Understanding the MMR mechanism can yield important insights into many aspects of human health, like the emergence of cancer and drug resistance in bacteria. To overcome experimental challenges with studying this process in living cells, we have developed a new method to enrich the rare genomic mutations by genotypic selection in a way that allows us to study the mismatch repair process in Escherichia coli, a model organism for MMR. We have shown the maximum 705,000-fold enrichment of DNA with a mutation even after a 10-6 times dilution by DNA with the wild-type sequence in vitro. After further optimization, we could then use this technique to directly measure MMR activity occurring in living E. coli. We expect this technique will open up new opportunities and research directions to study MMR-like processes in E. coli as well as different organisms, including Actinobacteria.
590 $a School code: 0154.
650 4 $a Nanoscience. $3 587832
650 4 $a Nanotechnology. $3 526235
653 $a Bacteria
653 $a Biotechnologies
653 $a Mismatch Repair
653 $a Rare Mutations
653 $a Study
690 $a 0565
690 $a 0652
710 2 $a The University of North Carolina at Greensboro. $b Joint School of Nanoscience and Nanoengineering. $3 3540476
773 0 $t Masters Abstracts International $g 82-01.
790 $a 0154
791 $a M.S.
792 $a 2020
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27833175