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Bioprocessing of Soybean Seed-Coats ...

Burkey, Carren Nyambare.

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Bioprocessing of Soybean Seed-Coats for Production of Proteins & Omega-3 Fatty Acids Using Pythium Isolates.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Bioprocessing of Soybean Seed-Coats for Production of Proteins & Omega-3 Fatty Acids Using Pythium Isolates./
Author:	Burkey, Carren Nyambare.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	58 p.
Notes:	Source: Masters Abstracts International, Volume: 82-05.
Contained By:	Masters Abstracts International82-05.
Subject:	Nutrition. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28186361
ISBN:	9798678158253

Bioprocessing of Soybean Seed-Coats for Production of Proteins & Omega-3 Fatty Acids Using Pythium Isolates.
Burkey, Carren Nyambare.

Bioprocessing of Soybean Seed-Coats for Production of Proteins & Omega-3 Fatty Acids Using Pythium Isolates. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 58 p.

Source: Masters Abstracts International, Volume: 82-05.

Thesis (M.S.)--Bowling Green State University, 2020.

This item must not be sold to any third party vendors.

Industrial processing of soybeans to produce soy oil and soy meal results in soybean seed coats as a low value waste product that is underutilized. Pythium are rapidly growing plant pathogens that secrete a large number of carbohydrate-digesting enzymes that possess the ability to breakdown the fibrous carbohydrates present in soybean seed coats to release the required carbon for their growth. We tap into this potential of oomycetes and utilize soybean seed coats as a source of carbon for the growth of Pythium under microaerobic, aerobic conditions and 4% sucrose supplementation to produce proteins and the important omega three and omega six fatty acids that can be included in fish feed as an alternative source of fish oil and proteins. Biomass produced by growing cultures on this carbohydrate source and inorganic N has a protein content exceeding 20% with a very favorable amino acid profile. Microaerobic culture conditions produce an unsaturated and saturated fatty acid mixture that mimics that of salmon and sardine oil. Aeration of samples and supplementation with 4% sucrose during the growth process significantly increases the amounts and variety of omega fatty acids produced. The results of this study suggest that soybean seed coats are a viable source of carbon for Pythium in the production of omega three and omega six fatty acids and proteins for aquaculture.

ISBN: 9798678158253Subjects--Topical Terms:

517777
Nutrition.
Subjects--Index Terms:

Omega 3 fatty acids

Bioprocessing of Soybean Seed-Coats for Production of Proteins & Omega-3 Fatty Acids Using Pythium Isolates.
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520 $a Industrial processing of soybeans to produce soy oil and soy meal results in soybean seed coats as a low value waste product that is underutilized. Pythium are rapidly growing plant pathogens that secrete a large number of carbohydrate-digesting enzymes that possess the ability to breakdown the fibrous carbohydrates present in soybean seed coats to release the required carbon for their growth. We tap into this potential of oomycetes and utilize soybean seed coats as a source of carbon for the growth of Pythium under microaerobic, aerobic conditions and 4% sucrose supplementation to produce proteins and the important omega three and omega six fatty acids that can be included in fish feed as an alternative source of fish oil and proteins. Biomass produced by growing cultures on this carbohydrate source and inorganic N has a protein content exceeding 20% with a very favorable amino acid profile. Microaerobic culture conditions produce an unsaturated and saturated fatty acid mixture that mimics that of salmon and sardine oil. Aeration of samples and supplementation with 4% sucrose during the growth process significantly increases the amounts and variety of omega fatty acids produced. The results of this study suggest that soybean seed coats are a viable source of carbon for Pythium in the production of omega three and omega six fatty acids and proteins for aquaculture.
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