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The Role of Hydrodynamics in Branchi...

Setru, Sagar Udayashankar.

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The Role of Hydrodynamics in Branching Microtubule Nucleation and the Role of Branching Microtubule Nucleation in Acentrosomal Spindle Assembly.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Role of Hydrodynamics in Branching Microtubule Nucleation and the Role of Branching Microtubule Nucleation in Acentrosomal Spindle Assembly./
Author:	Setru, Sagar Udayashankar.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	236 p.
Notes:	Source: Dissertations Abstracts International, Volume: 82-09, Section: B.
Contained By:	Dissertations Abstracts International82-09B.
Subject:	Biophysics. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28263649
ISBN:	9798582532590

The Role of Hydrodynamics in Branching Microtubule Nucleation and the Role of Branching Microtubule Nucleation in Acentrosomal Spindle Assembly.
Setru, Sagar Udayashankar.

The Role of Hydrodynamics in Branching Microtubule Nucleation and the Role of Branching Microtubule Nucleation in Acentrosomal Spindle Assembly. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 236 p.

Source: Dissertations Abstracts International, Volume: 82-09, Section: B.

Thesis (Ph.D.)--Princeton University, 2021.

This item must not be sold to any third party vendors.

Branching microtubule nucleation is key for properly assembling the spindle during eukaryotic cell division, yet much remains to be learned about how and where branched microtubules are nucleated in the cell to find and move chromosomes. To improve our understanding of how and where branching microtubule nucleation occurs and how the spindle moves chromosomes during cell division, I engineered reconstitutions to precisely isolate specific parts of cell division, reconstitutions that were both in vitro and ex vivo. Then, I tested various hypotheses concerning the physical and molecular mechanisms at play. In Chapter 2, we discovered that TPX2 undergoes the hydrodynamic Rayleigh-Plateau instability to form droplets on microtubules, droplets from which branched microtubules nucleate and which make branching nucleation more efficient. In Chapter 3, we discovered that chromosomes alone can generate spindles, and that branching microtubule nucleation is the chief source of microtubules generated at chromosomes. Ongoing work seeks to fit the experimentally measured distribution of microtubules over time to theory, specifically a model that predicts the architecture and dynamics of branched microtubule networks that assemble around chromosomes. In Chapter 4, we discovered how to reconstitute metaphase chromosome movement ex vivo in centrosomal microtubule asters and observed chromosomes moving poleward toward centrosomes, at a range of speeds similar to what has been observed in vivo. Ongoing work seeks to estimate the force applied on chromosomes by measuring the hydrodynamic radius of the chromosomes and then calculating the viscous drag opposing their motion.I also did work related to bacterial cell biology. In Chapter 5, using biochemistry, fluorescence microscopy, and mean-squared-displacement analysis, we discovered that the bacterial cytoskeleton spatially confines phase separated microdomains, also known as lipid rafts, within the bacterial membrane.

ISBN: 9798582532590Subjects--Topical Terms:

518360
Biophysics.
Subjects--Index Terms:

atomic force microscopy

The Role of Hydrodynamics in Branching Microtubule Nucleation and the Role of Branching Microtubule Nucleation in Acentrosomal Spindle Assembly.
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520 $a Branching microtubule nucleation is key for properly assembling the spindle during eukaryotic cell division, yet much remains to be learned about how and where branched microtubules are nucleated in the cell to find and move chromosomes. To improve our understanding of how and where branching microtubule nucleation occurs and how the spindle moves chromosomes during cell division, I engineered reconstitutions to precisely isolate specific parts of cell division, reconstitutions that were both in vitro and ex vivo. Then, I tested various hypotheses concerning the physical and molecular mechanisms at play. In Chapter 2, we discovered that TPX2 undergoes the hydrodynamic Rayleigh-Plateau instability to form droplets on microtubules, droplets from which branched microtubules nucleate and which make branching nucleation more efficient. In Chapter 3, we discovered that chromosomes alone can generate spindles, and that branching microtubule nucleation is the chief source of microtubules generated at chromosomes. Ongoing work seeks to fit the experimentally measured distribution of microtubules over time to theory, specifically a model that predicts the architecture and dynamics of branched microtubule networks that assemble around chromosomes. In Chapter 4, we discovered how to reconstitute metaphase chromosome movement ex vivo in centrosomal microtubule asters and observed chromosomes moving poleward toward centrosomes, at a range of speeds similar to what has been observed in vivo. Ongoing work seeks to estimate the force applied on chromosomes by measuring the hydrodynamic radius of the chromosomes and then calculating the viscous drag opposing their motion.I also did work related to bacterial cell biology. In Chapter 5, using biochemistry, fluorescence microscopy, and mean-squared-displacement analysis, we discovered that the bacterial cytoskeleton spatially confines phase separated microdomains, also known as lipid rafts, within the bacterial membrane.
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