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Effects of Microfluidic Mixing on th...

Piercy, Dennis Houston.

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Effects of Microfluidic Mixing on the Rheological and Structural Properties of Early-Phase Wounds.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Effects of Microfluidic Mixing on the Rheological and Structural Properties of Early-Phase Wounds./
Author:	Piercy, Dennis Houston.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	68 p.
Notes:	Source: Masters Abstracts International, Volume: 82-12.
Contained By:	Masters Abstracts International82-12.
Subject:	Chemical engineering. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28417300
ISBN:	9798516059377

Effects of Microfluidic Mixing on the Rheological and Structural Properties of Early-Phase Wounds.
Piercy, Dennis Houston.

Effects of Microfluidic Mixing on the Rheological and Structural Properties of Early-Phase Wounds. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 68 p.

Source: Masters Abstracts International, Volume: 82-12.

Thesis (M.S.)--Tennessee Technological University, 2021.

This item must not be sold to any third party vendors.

Dermal wound healing is a complex process that consists of multiple cascading reactions within the body that ideally results in a return to original function and structural integrity of the skin with minimal scarring. An integral part of this process is the formation of fibrin clots (or "hemostatic plugs") to restrict blood loss. The resulting fibrous network is the product of the enzymatic cleavage of fibrinogen by activated thrombin. The role of (micro)fluidic mixers to combine these components and produce uniform, or otherwise desired, wound gel structures is being investigated as described herein to give better control in experiments focused on exploring properties of these domains. Consistent gel formation reduces the amount of material required to run conclusive experimental tests since the structure and properties of these clots are dependent on the reagent concentrations used. Ultimately, designs of fluidic-based mixers are being explored. Specifically, four mixing strategies have been explored: 1) a diffusion-limited strategy in which the components are layered and allowed to slowly mix via diffusion, 2) a pipet mixing strategy involving a single pipetting maneuver, 3) mixing formed in a cured polydimethylsiloxane gel, and 4) mixing via a commercial micromixer (with internal channel diameter of 280 μm). During gel formation with each of these strategies, rheological characterizations, in the form of storage and loss moduli, were completed and in separate gels, turbidity measures were completed. Scanning electron microscopy (SEM) studies were also completed for fully-formed gels following freezing with liquid nitrogen, freeze-drying, mounting, and sputter coating with gold. A fifth mixing technique was explored using turbidity measurements and SEM imaging involving pipet mixing similar to the previously explained technique but with the pipetting maneuver repeated five times. The results reveal little difference in the rheological characteristics but pronounced differences in turbidity. SEM results point to potential influence of the preparation technique by possible differences in fiber size. Given the important role of structure on the function and performance of early-phase wound domains in enabling a desired wound healing outcome, research to identify and validate conditions for consistent gel formation and characterization is expected to increase confidence in experimental results and reduce the amount of the costly protein-based materials required to run conclusive experimental tests. Ultimately, in addition to the work summarized above, preliminary data and problems encountered in using an ultra-small microfluidic-based mixer (with internal volume of 4.1 μl) is also presented.

ISBN: 9798516059377Subjects--Topical Terms:

560457
Chemical engineering.
Subjects--Index Terms:

Coagulation

Effects of Microfluidic Mixing on the Rheological and Structural Properties of Early-Phase Wounds.
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300 $a 68 p.
500 $a Source: Masters Abstracts International, Volume: 82-12.
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520 $a Dermal wound healing is a complex process that consists of multiple cascading reactions within the body that ideally results in a return to original function and structural integrity of the skin with minimal scarring. An integral part of this process is the formation of fibrin clots (or "hemostatic plugs") to restrict blood loss. The resulting fibrous network is the product of the enzymatic cleavage of fibrinogen by activated thrombin. The role of (micro)fluidic mixers to combine these components and produce uniform, or otherwise desired, wound gel structures is being investigated as described herein to give better control in experiments focused on exploring properties of these domains. Consistent gel formation reduces the amount of material required to run conclusive experimental tests since the structure and properties of these clots are dependent on the reagent concentrations used. Ultimately, designs of fluidic-based mixers are being explored. Specifically, four mixing strategies have been explored: 1) a diffusion-limited strategy in which the components are layered and allowed to slowly mix via diffusion, 2) a pipet mixing strategy involving a single pipetting maneuver, 3) mixing formed in a cured polydimethylsiloxane gel, and 4) mixing via a commercial micromixer (with internal channel diameter of 280 μm). During gel formation with each of these strategies, rheological characterizations, in the form of storage and loss moduli, were completed and in separate gels, turbidity measures were completed. Scanning electron microscopy (SEM) studies were also completed for fully-formed gels following freezing with liquid nitrogen, freeze-drying, mounting, and sputter coating with gold. A fifth mixing technique was explored using turbidity measurements and SEM imaging involving pipet mixing similar to the previously explained technique but with the pipetting maneuver repeated five times. The results reveal little difference in the rheological characteristics but pronounced differences in turbidity. SEM results point to potential influence of the preparation technique by possible differences in fiber size. Given the important role of structure on the function and performance of early-phase wound domains in enabling a desired wound healing outcome, research to identify and validate conditions for consistent gel formation and characterization is expected to increase confidence in experimental results and reduce the amount of the costly protein-based materials required to run conclusive experimental tests. Ultimately, in addition to the work summarized above, preliminary data and problems encountered in using an ultra-small microfluidic-based mixer (with internal volume of 4.1 μl) is also presented.
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653 $a Microfluidics
653 $a Thrombin
653 $a Turbidity
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