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Plasmonic Enhancement and Sensing us...

Das, Ananda.

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Plasmonic Enhancement and Sensing using Upconversion Nanoparticles.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Plasmonic Enhancement and Sensing using Upconversion Nanoparticles./
作者:	Das, Ananda.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	138 p.
附註:	Source: Dissertations Abstracts International, Volume: 82-12, Section: B.
Contained By:	Dissertations Abstracts International82-12B.
標題:	Nanoscience. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28415816
ISBN:	9798515243272

Plasmonic Enhancement and Sensing using Upconversion Nanoparticles.
Das, Ananda.

Plasmonic Enhancement and Sensing using Upconversion Nanoparticles. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 138 p.

Source: Dissertations Abstracts International, Volume: 82-12, Section: B.

Thesis (Ph.D.)--University of Colorado at Boulder, 2021.

This item must not be sold to any third party vendors.

This thesis reports on the photoluminescence modulation of upconversion nanoparticles (UCNPs) using plasmonic nanostructures and fluorescent dyes for applications in bioimaging and sensing.I start by demonstrating plasmonic enhancement of UCNPs using a metal-insulator-metal (MIM) nanostructure. The MIM nanostructure supports a highly localized surface plasmon mode at the absorption wavelength of the UCNPs. The structure can be lifted off the substrate and dispersed into water for bioimaging experiments. UCNP emission is enhanced by over three orders of magnitude when using the MIM nanostructure. As a proof of principle demonstration, the MIMs are successfully used to image cancer cells.Next, I model the effects of Forster resonant energy transfer (FRET) in a dye-conjugated UCNP sensing system. In these systems, sensing can be achieved by the modulation of FRET between the dye and the UCNP. The effects of FRET in such cases are complex as the extent to which FRET is experienced by the rare-earth ions is dependent on their position within the nanoparticle. I develop an analytical model to accurately describe the effects of FRET for such a system. The model is verified using a pH sensor comprised of Fluorescein Isothiocyanate (FITC) and thulium (Tm3+) doped UCNPs. I extend the model to the case of core-shell UCNPs and discuss the design of an optimal FRET-based biosensor using UCNPs. Finally, I propose a novel approach to quantitative sensing of pH using the FITC - Tm3+ UCNP sensing system. I analyze the upconversion mechanism in Tm3+ UCNPs and show that it is possible to design a FRET-based ratiometric sensor where the two wavelengths measured have no overlap with the dye absorption spectrum. This system can be used to realize an ideal quantitative sensor where a calibration curve established in a lab serves as a valid reference for in vivo and in vitro measurements. Sensitivity to pH is demonstrated experimentally and the system is found to be superior to current techniques reported in the literature that use a photon reabsorption-based approach.

ISBN: 9798515243272Subjects--Topical Terms:

587832
Nanoscience.
Subjects--Index Terms:

Plasmonic enhancement

Plasmonic Enhancement and Sensing using Upconversion Nanoparticles.
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500 $a Source: Dissertations Abstracts International, Volume: 82-12, Section: B.
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506 $a This item must not be sold to any third party vendors.
520 $a This thesis reports on the photoluminescence modulation of upconversion nanoparticles (UCNPs) using plasmonic nanostructures and fluorescent dyes for applications in bioimaging and sensing.I start by demonstrating plasmonic enhancement of UCNPs using a metal-insulator-metal (MIM) nanostructure. The MIM nanostructure supports a highly localized surface plasmon mode at the absorption wavelength of the UCNPs. The structure can be lifted off the substrate and dispersed into water for bioimaging experiments. UCNP emission is enhanced by over three orders of magnitude when using the MIM nanostructure. As a proof of principle demonstration, the MIMs are successfully used to image cancer cells.Next, I model the effects of Forster resonant energy transfer (FRET) in a dye-conjugated UCNP sensing system. In these systems, sensing can be achieved by the modulation of FRET between the dye and the UCNP. The effects of FRET in such cases are complex as the extent to which FRET is experienced by the rare-earth ions is dependent on their position within the nanoparticle. I develop an analytical model to accurately describe the effects of FRET for such a system. The model is verified using a pH sensor comprised of Fluorescein Isothiocyanate (FITC) and thulium (Tm3+) doped UCNPs. I extend the model to the case of core-shell UCNPs and discuss the design of an optimal FRET-based biosensor using UCNPs. Finally, I propose a novel approach to quantitative sensing of pH using the FITC - Tm3+ UCNP sensing system. I analyze the upconversion mechanism in Tm3+ UCNPs and show that it is possible to design a FRET-based ratiometric sensor where the two wavelengths measured have no overlap with the dye absorption spectrum. This system can be used to realize an ideal quantitative sensor where a calibration curve established in a lab serves as a valid reference for in vivo and in vitro measurements. Sensitivity to pH is demonstrated experimentally and the system is found to be superior to current techniques reported in the literature that use a photon reabsorption-based approach.
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