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The Development of Multifunctional S...

Zhu, Cheng.

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The Development of Multifunctional Scanning Ion Conductance Microscopy for Local Physical Property Measurements.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Development of Multifunctional Scanning Ion Conductance Microscopy for Local Physical Property Measurements./
Author:	Zhu, Cheng.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
Description:	187 p.
Notes:	Source: Dissertations Abstracts International, Volume: 82-09, Section: B.
Contained By:	Dissertations Abstracts International82-09B.
Subject:	Analytical chemistry. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28316677
ISBN:	9798582507086

The Development of Multifunctional Scanning Ion Conductance Microscopy for Local Physical Property Measurements.
Zhu, Cheng.

The Development of Multifunctional Scanning Ion Conductance Microscopy for Local Physical Property Measurements. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 187 p.

Source: Dissertations Abstracts International, Volume: 82-09, Section: B.

Thesis (Ph.D.)--Indiana University, 2021.

This item must not be sold to any third party vendors.

Nanoscale measurement of local physical properties is of great importance for the investigation of biological samples. Although this remains difficult for most conventional techniques, developments in scanning ion conductance microscopy (SICM) provide alternate methods to achieve multifunctional measurements. A typical instrument setup for SICM includes a pipette electrode inserted inside an electrolyte-filled pipette and another reference electrode immersed in a bulk electrolyte. Feedback is established by the distance-dependent ion current that flows between these electrodes and the topography is acquired by laterally scanning the probe over the sample surface. With this configuration, electrochemical measurements can be easily incorporated into SICM measurements and as a result, additional physical/chemical properties can be mapped simultaneously during acquisition of topographic scans.In this thesis, we first demonstrated the capability of SICM for topographic measurement of complex and delicate biological samples, namely in monitoring dynamic membrane processes in red blood cells (RBCs). Several advanced SICM platforms were subsequently described and applied to the mapping of surface charge and local potential. Local surface charge was measured by incorporating current-voltage (I-V) measurements into scanning, with differential surface measurements referenced to bulk solution was used to interpret the distribution of local surface charges. This technique was first demonstrated with silane functionalized substrates and then applied in the investigations of natural mineral samples. Potential mapping was achieved through incorporation of voltage-gated ion channels in a dual-barrel pipette to form an ion channel probe (ICP). The pipette was then used as the probe for ICP-SICM measurement, where amperometric current responses (I-T) of the ion channels were measured at each measurement point and fitted into a pre-collected calibration curve to quantify the local potential magnitudes over porous substrates.

ISBN: 9798582507086Subjects--Topical Terms:

3168300
Analytical chemistry.
Subjects--Index Terms:

Multifunctional scanning

The Development of Multifunctional Scanning Ion Conductance Microscopy for Local Physical Property Measurements.
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300 $a 187 p.
500 $a Source: Dissertations Abstracts International, Volume: 82-09, Section: B.
500 $a Advisor: Baker, Lane.
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506 $a This item must not be sold to any third party vendors.
520 $a Nanoscale measurement of local physical properties is of great importance for the investigation of biological samples. Although this remains difficult for most conventional techniques, developments in scanning ion conductance microscopy (SICM) provide alternate methods to achieve multifunctional measurements. A typical instrument setup for SICM includes a pipette electrode inserted inside an electrolyte-filled pipette and another reference electrode immersed in a bulk electrolyte. Feedback is established by the distance-dependent ion current that flows between these electrodes and the topography is acquired by laterally scanning the probe over the sample surface. With this configuration, electrochemical measurements can be easily incorporated into SICM measurements and as a result, additional physical/chemical properties can be mapped simultaneously during acquisition of topographic scans.In this thesis, we first demonstrated the capability of SICM for topographic measurement of complex and delicate biological samples, namely in monitoring dynamic membrane processes in red blood cells (RBCs). Several advanced SICM platforms were subsequently described and applied to the mapping of surface charge and local potential. Local surface charge was measured by incorporating current-voltage (I-V) measurements into scanning, with differential surface measurements referenced to bulk solution was used to interpret the distribution of local surface charges. This technique was first demonstrated with silane functionalized substrates and then applied in the investigations of natural mineral samples. Potential mapping was achieved through incorporation of voltage-gated ion channels in a dual-barrel pipette to form an ion channel probe (ICP). The pipette was then used as the probe for ICP-SICM measurement, where amperometric current responses (I-T) of the ion channels were measured at each measurement point and fitted into a pre-collected calibration curve to quantify the local potential magnitudes over porous substrates.
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653 $a Nanoscale measurement
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