東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Tissue-Engineered Nanoclay-Based Bon...

Kar, Sumanta .

Linked to FindBook

Google Book

Amazon

博客來

Tissue-Engineered Nanoclay-Based Bone-Mimetic 3D in Vitro Testbed for Studying Breast Cancer Metastasis to Bone.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Tissue-Engineered Nanoclay-Based Bone-Mimetic 3D in Vitro Testbed for Studying Breast Cancer Metastasis to Bone./
Author:	Kar, Sumanta .
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	352 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-11, Section: B.
Contained By:	Dissertations Abstracts International81-11B.
Subject:	Bioengineering. -
Online resource:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27955926
ISBN:	9798644902163

Tissue-Engineered Nanoclay-Based Bone-Mimetic 3D in Vitro Testbed for Studying Breast Cancer Metastasis to Bone.
Kar, Sumanta .

Tissue-Engineered Nanoclay-Based Bone-Mimetic 3D in Vitro Testbed for Studying Breast Cancer Metastasis to Bone. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 352 p.

Source: Dissertations Abstracts International, Volume: 81-11, Section: B.

Thesis (Ph.D.)--North Dakota State University, 2020.

This item must not be sold to any third party vendors.

Breast cancer shows a high affinity towards the bone, causing bone-related complications leading to poor clinical prognosis. Approximately 80% of breast cancer patients die within five years after primary cancer has metastasized to the bones. The tumor stage strongly influences the survival rates of patients with breast cancer that has spread to bone at the time of diagnosis. There are currently no effective therapeutics available for bone metastases due to the failure of animal models and the scarcity of human bone metastasized samples, as most patients with advance stages of cancer are already in palliative care. Therefore, it is imperative to develop translational models to elucidate disease mechanisms at the cellular and molecular level. Here, we report the development of tissue-engineered nanoclay-based bone-mimetic three-dimensional (3D) in vitro model for studying later stages of cancer pathogenesis at the metastatic bone site using osteogenically-differentiated human mesenchymal stem cells (MSCs) and human breast cancer cells (MDA-MB-231 and MCF-7). This 3D model provides an ideal microenvironment suitable for cell-cell and cell-matrix interactions while retaining the behavior of breast cancer cells with different metastatic potential along with mimicking mesenchymal to epithelial transition (MET) of breast cancer cells. Sequential cultures of MSCs with MCF-7 gave rise to tumoroids, while sequential cultures of MSCs with MDA-MB-231 formed disorganized clusters of cells with poor cell-cell adhesion. We further evaluated how cancer-derived factors and cytokines affect bone leading to up to metastasis and conferring drug resistance, respectively. Results showed that Wnt/β-catenin and interleukin-6 (IL-6) mediated IL-6/STAT3 pathways are responsible for bone-related complications and conferring drug resistance, respectively. Furthermore, we have utilized the 3D in vitro model to develop methods for non-invasive and rapid prediction of cancer progression using various biophysical techniques such as spectroscopy and nanoindentation. Spectroscopy methods showed significant contributions of proteins, lipids, and nucleic acids, while the nanoindentation method showed F-actin mediated softening of cancer cells during cancer progression at the metastatic bone site, respectively. Collectively, 3D in vitro model provides an ideal platform for studying the molecular mechanism of breast cancer progression at the metastatic bone site, drug development, and discovery of biomarkers for cancer progression.

ISBN: 9798644902163Subjects--Topical Terms:

657580
Bioengineering.
Subjects--Index Terms:

Bone Metastasis

Tissue-Engineered Nanoclay-Based Bone-Mimetic 3D in Vitro Testbed for Studying Breast Cancer Metastasis to Bone.
LDR:03731nmm a2200373 4500 001 2276842
005 20210510092423.5
008 220723s2020 ||||||||||||||||| ||eng d
020 $a 9798644902163
035 $a (MiAaPQ)AAI27955926
035 $a AAI27955926
040 $a MiAaPQ $c MiAaPQ
100 1 $a Kar, Sumanta . $3 3555144
245 1 0 $a Tissue-Engineered Nanoclay-Based Bone-Mimetic 3D in Vitro Testbed for Studying Breast Cancer Metastasis to Bone.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 352 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-11, Section: B.
500 $a Advisor: Katti, Kalpana S.
502 $a Thesis (Ph.D.)--North Dakota State University, 2020.
506 $a This item must not be sold to any third party vendors.
520 $a Breast cancer shows a high affinity towards the bone, causing bone-related complications leading to poor clinical prognosis. Approximately 80% of breast cancer patients die within five years after primary cancer has metastasized to the bones. The tumor stage strongly influences the survival rates of patients with breast cancer that has spread to bone at the time of diagnosis. There are currently no effective therapeutics available for bone metastases due to the failure of animal models and the scarcity of human bone metastasized samples, as most patients with advance stages of cancer are already in palliative care. Therefore, it is imperative to develop translational models to elucidate disease mechanisms at the cellular and molecular level. Here, we report the development of tissue-engineered nanoclay-based bone-mimetic three-dimensional (3D) in vitro model for studying later stages of cancer pathogenesis at the metastatic bone site using osteogenically-differentiated human mesenchymal stem cells (MSCs) and human breast cancer cells (MDA-MB-231 and MCF-7). This 3D model provides an ideal microenvironment suitable for cell-cell and cell-matrix interactions while retaining the behavior of breast cancer cells with different metastatic potential along with mimicking mesenchymal to epithelial transition (MET) of breast cancer cells. Sequential cultures of MSCs with MCF-7 gave rise to tumoroids, while sequential cultures of MSCs with MDA-MB-231 formed disorganized clusters of cells with poor cell-cell adhesion. We further evaluated how cancer-derived factors and cytokines affect bone leading to up to metastasis and conferring drug resistance, respectively. Results showed that Wnt/β-catenin and interleukin-6 (IL-6) mediated IL-6/STAT3 pathways are responsible for bone-related complications and conferring drug resistance, respectively. Furthermore, we have utilized the 3D in vitro model to develop methods for non-invasive and rapid prediction of cancer progression using various biophysical techniques such as spectroscopy and nanoindentation. Spectroscopy methods showed significant contributions of proteins, lipids, and nucleic acids, while the nanoindentation method showed F-actin mediated softening of cancer cells during cancer progression at the metastatic bone site, respectively. Collectively, 3D in vitro model provides an ideal platform for studying the molecular mechanism of breast cancer progression at the metastatic bone site, drug development, and discovery of biomarkers for cancer progression.
590 $a School code: 0157.
650 4 $a Bioengineering. $3 657580
650 4 $a Biochemistry. $3 518028
650 4 $a Materials science. $3 543314
653 $a Bone Metastasis
653 $a Breast Cancer
653 $a In Vitro
653 $a Nanoclay
653 $a Tissue Engineering
690 $a 0794
690 $a 0202
690 $a 0487
710 2 $a North Dakota State University. $b Materials and Nanotechnology. $3 3282448
773 0 $t Dissertations Abstracts International $g 81-11B.
790 $a 0157
791 $a Ph.D.
792 $a 2020
793 $a English
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27955926