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Papertronic Sensing Arrays for Rapid...
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Tahernia, Mehdi.
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Papertronic Sensing Arrays for Rapid and High-Throughput Screening of Electroactive Microorganisms.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Papertronic Sensing Arrays for Rapid and High-Throughput Screening of Electroactive Microorganisms./
作者:
Tahernia, Mehdi.
出版者:
Ann Arbor : ProQuest Dissertations & Theses, : 2020,
面頁冊數:
110 p.
附註:
Source: Dissertations Abstracts International, Volume: 82-04, Section: B.
Contained By:
Dissertations Abstracts International82-04B.
標題:
Electrical engineering. -
電子資源:
https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28027347
ISBN:
9798678160959
Papertronic Sensing Arrays for Rapid and High-Throughput Screening of Electroactive Microorganisms.
Tahernia, Mehdi.
Papertronic Sensing Arrays for Rapid and High-Throughput Screening of Electroactive Microorganisms.
- Ann Arbor : ProQuest Dissertations & Theses, 2020 - 110 p.
Source: Dissertations Abstracts International, Volume: 82-04, Section: B.
Thesis (Ph.D.)--State University of New York at Binghamton, 2020.
This item must not be sold to any third party vendors.
Electromicrobiology, which uses the electricity-producing capability, or "electrogenicity" of bacteria, promises novel technologies that address pressing societal concerns about energy security, environmental protection and remediation, and economic development. The bidirectional bacterial electron exchange generates environmentally sustainable bioelectricity from organic waste. The process produces value-added chemicals, or biofuels, and can perform many other environmentally important functions, such as water desalination, bioremediation, and toxicity detection. These bacterial capabilities have entered a new phase of development with biotechniques in synthetic biology that genetically regulate bacterial metabolic pathways and improve their electrogenic potential. Microbial synthetic biology will develop a fundamentally different strategy to advance electromicrobiology by maximizing the inherent electron-transferring capability of bacteria, translating the technology from laboratory novelty to practical applications. What is needed more than ever is a sensing technique for characterizing the electrogenicity of many of the newly discovered, genetically engineered bacteria. This dissertation work creates the ability to achieve rapid, sensitive, and high-throughput characterization of bacterial electrogenicity from a single drop of culture. Through an innovative microscale microbial fuel cell (MFC) structure integrated into paper, a high-throughput but simple, capillary driven sensing array can be constructed, resulting in the rapid and sensitive power assessment of electrogenic bacteria from a microliter sample volume. Chapter 1 discusses an overview of the fundamental principles behind electromicrobiology. Then, 64-well and 96-well papertronic sensing arrays are demonstrated in Chapter 2 and Chapter 3, respectively. In Chapter 4, a 21-well paper-based MFC array with enhanced sensitivity is developed as a powerful yet simple screening method to accurately and simultaneously characterize electrogenic capabilities of human gut microbes. Chapter 5 shows a more simplified papertronic sensing system for rapid visual screening of bacterial electrogenicity. Chapter 6 creates a papertronic three-electrode potentiostat to provide a more controllable analytic capability without unwanted perturbations. Finally, a comprehensive summary and outlook for the sensing arrays is provided.
ISBN: 9798678160959Subjects--Topical Terms:
649834
Electrical engineering.
Subjects--Index Terms:
Bidirectional bacterial electron exchange
Papertronic Sensing Arrays for Rapid and High-Throughput Screening of Electroactive Microorganisms.
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Electromicrobiology, which uses the electricity-producing capability, or "electrogenicity" of bacteria, promises novel technologies that address pressing societal concerns about energy security, environmental protection and remediation, and economic development. The bidirectional bacterial electron exchange generates environmentally sustainable bioelectricity from organic waste. The process produces value-added chemicals, or biofuels, and can perform many other environmentally important functions, such as water desalination, bioremediation, and toxicity detection. These bacterial capabilities have entered a new phase of development with biotechniques in synthetic biology that genetically regulate bacterial metabolic pathways and improve their electrogenic potential. Microbial synthetic biology will develop a fundamentally different strategy to advance electromicrobiology by maximizing the inherent electron-transferring capability of bacteria, translating the technology from laboratory novelty to practical applications. What is needed more than ever is a sensing technique for characterizing the electrogenicity of many of the newly discovered, genetically engineered bacteria. This dissertation work creates the ability to achieve rapid, sensitive, and high-throughput characterization of bacterial electrogenicity from a single drop of culture. Through an innovative microscale microbial fuel cell (MFC) structure integrated into paper, a high-throughput but simple, capillary driven sensing array can be constructed, resulting in the rapid and sensitive power assessment of electrogenic bacteria from a microliter sample volume. Chapter 1 discusses an overview of the fundamental principles behind electromicrobiology. Then, 64-well and 96-well papertronic sensing arrays are demonstrated in Chapter 2 and Chapter 3, respectively. In Chapter 4, a 21-well paper-based MFC array with enhanced sensitivity is developed as a powerful yet simple screening method to accurately and simultaneously characterize electrogenic capabilities of human gut microbes. Chapter 5 shows a more simplified papertronic sensing system for rapid visual screening of bacterial electrogenicity. Chapter 6 creates a papertronic three-electrode potentiostat to provide a more controllable analytic capability without unwanted perturbations. Finally, a comprehensive summary and outlook for the sensing arrays is provided.
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