東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Development of Functionalized Capill...

Trang, Hung Khiem.

FindBook

Google Book

Amazon

博客來

Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography./
作者:	Trang, Hung Khiem.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:	270 p.
附註:	Source: Dissertations Abstracts International, Volume: 81-09, Section: B.
Contained By:	Dissertations Abstracts International81-09B.
標題:	Chemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27667624
ISBN:	9781392827932

Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography.
Trang, Hung Khiem.

Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 270 p.

Source: Dissertations Abstracts International, Volume: 81-09, Section: B.

Thesis (Ph.D.)--Clemson University, 2019.

This item must not be sold to any third party vendors.

At the heart of the chromatography technique, the stationary phase is the essential component that dictates multiple aspects of the separation process. Diverse chemistry of the stationary phases, in combination with the choice of appropriate base support materials, allows chromatography to be used in a wide range of applications ranging from the high-performance separations of trace analytes in complex biological samples for chemical detection and quantification to the large-scale purification of a recombinant protein from a complex media. Traditional chromatographic stationary phases mostly utilize porous materials for the high surface area and versatility for chemical modification. However, in many cases, these materials suffer from high operating back-pressure and inefficient mass transfer. Joining the efforts to investigate alternative phases, Marcus research group (Clemson University) has investigated the potentials of capillary-channeled polymer (C-CP) fibers for the past 15 years. These fibers are characterized by eight capillary channels running down the length of the fiber, allowing the fibers to interdigitate to form well-aligned micrometer-sized channels when packed into a column format. It is the unique shape that allows separations to be conducted at high linear velocities (>75 mm s−1) with high column permeability (<0.14 MPa cm−1). The highly efficient fluid movement through the narrow channels composed of non-porous C-CP fibers gives rise to favorable mass transfer rates, facilitating fast protein separations and processing. Moreover, another advantage of C-CP fiber supports over other commercial sorbents is that these fibers are quite stable over a wide pH range and are fairly inexpensive (<$ 100 lb−1). Additionally, packing a C-CP fiber column is a simple process that can be conveniently performed without any special equipment.The concepts of using natural and synthetic polymer fibers as chromatographic stationary phases are not new. Potential advantages cited include low costs, ease in column fabrication, low operation backpressure, facile solute mass transfer, and a general ease of tailoring fiber surfaces to affect high levels of chemical specificity. There have been several surface modification techniques reported for polymer fibers in the literature. However, many of these modification methods can be detrimental to the physical properties of the polymer by destroying the polymer backbone. In order to further exploit the advantageous fluidic properties of C-CP fiber columns without compromising the fiber integrity, milder modification approaches have been a focus of this group recently, including covalent coupling and direct ligand adsorption methods. The research studies reported in this work focus on the development of functionalized C-CP fiber for a variety of affinity chromatography applications. The ultimate goal is to investigate non-invasive modification schemes that do not compromise the mechanical strength and fluidic properties of C-CP fibers. Affinity ligands are immobilized on the fiber surface through one of the following schemes:1. Scheme 1: Physical adsorption of recombinant protein A (rSPA) ligands on polypropylene (PP) C-CP fibers for immunoglobulin (IgG) capture2. Scheme 2: Microwave-assisted grafting polymerization of Glycidyl Methacrylate (GMA) onto nylon C-CP fibers as a ligand binding platform with applications in Immobilized Metal-ion Affinity Chromatography (IMAC) protein separations and uranium capture3. Scheme 3: Polydopamine-coated C-CP fiber for phosphopeptides capture and analyses.

ISBN: 9781392827932Subjects--Topical Terms:

516420
Chemistry.
Subjects--Index Terms:

Affinity

Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography.
LDR:04700nmm a2200337 4500 001 2273292
005 20201109125212.5
008 220629s2019 ||||||||||||||||| ||eng d
020 $a 9781392827932
035 $a (MiAaPQ)AAI27667624
035 $a AAI27667624
040 $a MiAaPQ $c MiAaPQ
100 1 $a Trang, Hung Khiem. $3 3550728
245 1 0 $a Development of Functionalized Capillary-Channeled Polymer (C-CP) Fibers as Stationary Phase for Affinity Chromatography.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 270 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-09, Section: B.
500 $a Advisor: Marcus, R. Kenneth.
502 $a Thesis (Ph.D.)--Clemson University, 2019.
506 $a This item must not be sold to any third party vendors.
520 $a At the heart of the chromatography technique, the stationary phase is the essential component that dictates multiple aspects of the separation process. Diverse chemistry of the stationary phases, in combination with the choice of appropriate base support materials, allows chromatography to be used in a wide range of applications ranging from the high-performance separations of trace analytes in complex biological samples for chemical detection and quantification to the large-scale purification of a recombinant protein from a complex media. Traditional chromatographic stationary phases mostly utilize porous materials for the high surface area and versatility for chemical modification. However, in many cases, these materials suffer from high operating back-pressure and inefficient mass transfer. Joining the efforts to investigate alternative phases, Marcus research group (Clemson University) has investigated the potentials of capillary-channeled polymer (C-CP) fibers for the past 15 years. These fibers are characterized by eight capillary channels running down the length of the fiber, allowing the fibers to interdigitate to form well-aligned micrometer-sized channels when packed into a column format. It is the unique shape that allows separations to be conducted at high linear velocities (>75 mm s−1) with high column permeability (<0.14 MPa cm−1). The highly efficient fluid movement through the narrow channels composed of non-porous C-CP fibers gives rise to favorable mass transfer rates, facilitating fast protein separations and processing. Moreover, another advantage of C-CP fiber supports over other commercial sorbents is that these fibers are quite stable over a wide pH range and are fairly inexpensive (<$ 100 lb−1). Additionally, packing a C-CP fiber column is a simple process that can be conveniently performed without any special equipment.The concepts of using natural and synthetic polymer fibers as chromatographic stationary phases are not new. Potential advantages cited include low costs, ease in column fabrication, low operation backpressure, facile solute mass transfer, and a general ease of tailoring fiber surfaces to affect high levels of chemical specificity. There have been several surface modification techniques reported for polymer fibers in the literature. However, many of these modification methods can be detrimental to the physical properties of the polymer by destroying the polymer backbone. In order to further exploit the advantageous fluidic properties of C-CP fiber columns without compromising the fiber integrity, milder modification approaches have been a focus of this group recently, including covalent coupling and direct ligand adsorption methods. The research studies reported in this work focus on the development of functionalized C-CP fiber for a variety of affinity chromatography applications. The ultimate goal is to investigate non-invasive modification schemes that do not compromise the mechanical strength and fluidic properties of C-CP fibers. Affinity ligands are immobilized on the fiber surface through one of the following schemes:1. Scheme 1: Physical adsorption of recombinant protein A (rSPA) ligands on polypropylene (PP) C-CP fibers for immunoglobulin (IgG) capture2. Scheme 2: Microwave-assisted grafting polymerization of Glycidyl Methacrylate (GMA) onto nylon C-CP fibers as a ligand binding platform with applications in Immobilized Metal-ion Affinity Chromatography (IMAC) protein separations and uranium capture3. Scheme 3: Polydopamine-coated C-CP fiber for phosphopeptides capture and analyses.
590 $a School code: 0050.
650 4 $a Chemistry. $3 516420
650 4 $a Polymer chemistry. $3 3173488
653 $a Affinity
653 $a Chromatography
653 $a Fiber
690 $a 0485
690 $a 0495
710 2 $a Clemson University. $b Chemistry. $3 1030764
773 0 $t Dissertations Abstracts International $g 81-09B.
790 $a 0050
791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27667624