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Integrated Continuous Biomanufacturi...

Kamga, Mark Henry.

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Integrated Continuous Biomanufacturing: Process Optimization, Future Considerations and Epigenetic Impacts on Long-term Culture.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Integrated Continuous Biomanufacturing: Process Optimization, Future Considerations and Epigenetic Impacts on Long-term Culture./
Author:	Kamga, Mark Henry.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	140 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Contained By:	Dissertations Abstracts International81-04B.
Subject:	Bioengineering. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=22589864
ISBN:	9781088375402

Integrated Continuous Biomanufacturing: Process Optimization, Future Considerations and Epigenetic Impacts on Long-term Culture.
Kamga, Mark Henry.

Integrated Continuous Biomanufacturing: Process Optimization, Future Considerations and Epigenetic Impacts on Long-term Culture. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 140 p.

Source: Dissertations Abstracts International, Volume: 81-04, Section: B.

Thesis (Ph.D.)--University of Massachusetts Lowell, 2019.

This item must not be sold to any third party vendors.

Integrated continuous biomanufacturing (ICB) has gained significant prominence over the past decade for the production of therapeutic proteins. ICB offers many advantages over traditional batch and fed-batch processes and successful implementation and expansion of this method of production has the potential to reduce cost and improve product safety and efficacy. However, to date, despite many advances in the field, in order to successfully expand this platform process, several factors still need to be addressed. In this work, we briefly discuss some challenges associated with ICB and present some novel contributions from our group towards improving ICB. Firstly, we demonstrate a novel and successful approach for performing process optimization in ICB by performing an in-process design of experiment (DOE) in continuous cell culture operations. We equally present a simplified ICB using a single-column capture step designed to simplify ICB processes and permit wider implementation. Secondly, we present a comprehensive overview of genetic and epigenetic considerations for a successful ICB process from cell line development, clone selection, instability assessment and process and media considerations. Lastly, we present a global genome analysis of epigenetic data for understanding the impact of epigenetic factors on ICB process outcomes. Our data will be useful for implementing a rapid, high throughput method for understanding cell line instability and will be useful for cell line development and process design in ICB processes. The implementation of the findings and recommendations from our work will contribute towards addressing some of the problems associated with ICB with the goal of permitting a wider implementation. This will invariably cut cost, improve patient safety and guarantee consistent supply of important therapeutic proteins globally.

ISBN: 9781088375402Subjects--Topical Terms:

657580
Bioengineering.
Subjects--Index Terms:

Biomanufacturing

Integrated Continuous Biomanufacturing: Process Optimization, Future Considerations and Epigenetic Impacts on Long-term Culture.
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500 $a Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
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506 $a This item must not be sold to any third party vendors.
520 $a Integrated continuous biomanufacturing (ICB) has gained significant prominence over the past decade for the production of therapeutic proteins. ICB offers many advantages over traditional batch and fed-batch processes and successful implementation and expansion of this method of production has the potential to reduce cost and improve product safety and efficacy. However, to date, despite many advances in the field, in order to successfully expand this platform process, several factors still need to be addressed. In this work, we briefly discuss some challenges associated with ICB and present some novel contributions from our group towards improving ICB. Firstly, we demonstrate a novel and successful approach for performing process optimization in ICB by performing an in-process design of experiment (DOE) in continuous cell culture operations. We equally present a simplified ICB using a single-column capture step designed to simplify ICB processes and permit wider implementation. Secondly, we present a comprehensive overview of genetic and epigenetic considerations for a successful ICB process from cell line development, clone selection, instability assessment and process and media considerations. Lastly, we present a global genome analysis of epigenetic data for understanding the impact of epigenetic factors on ICB process outcomes. Our data will be useful for implementing a rapid, high throughput method for understanding cell line instability and will be useful for cell line development and process design in ICB processes. The implementation of the findings and recommendations from our work will contribute towards addressing some of the problems associated with ICB with the goal of permitting a wider implementation. This will invariably cut cost, improve patient safety and guarantee consistent supply of important therapeutic proteins globally.
590 $a School code: 0111.
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653 $a Continuous purification
653 $a Epigenetics
653 $a Integrated continuous biomanufacting
653 $a Perfusion cell culture
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