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Characterization of the Effects of S...

Jamadar, Sakha .

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Characterization of the Effects of Selenium on the Lipid Profile Induced by the Environmental Pollutant Phenylmercury Acetate Exposure in Caenorhabditis elegans.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization of the Effects of Selenium on the Lipid Profile Induced by the Environmental Pollutant Phenylmercury Acetate Exposure in Caenorhabditis elegans./
Author:	Jamadar, Sakha .
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	90 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-08, Section: B.
Contained By:	Dissertations Abstracts International81-08B.
Subject:	Environmental science. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27668331
ISBN:	9781392710180

Characterization of the Effects of Selenium on the Lipid Profile Induced by the Environmental Pollutant Phenylmercury Acetate Exposure in Caenorhabditis elegans.
Jamadar, Sakha .

Characterization of the Effects of Selenium on the Lipid Profile Induced by the Environmental Pollutant Phenylmercury Acetate Exposure in Caenorhabditis elegans. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 90 p.

Source: Dissertations Abstracts International, Volume: 81-08, Section: B.

Thesis (Ph.D.)--Texas Southern University, 2019.

This item must not be sold to any third party vendors.

The free-living nematode Caenorhabditis elegans is a proven model organism for lipid metabolism research. Experimental advantages of the C. elegans model include a fast- reproductive cycle, known cell lineages, and a fully sequenced genome. C. elegans studies showed that chronic exposure to mercury (Hg) compounds induces neuron degeneration likely due to the increase in reactive oxygen species (ROS). Selenium (Se) is an essential trace element required for activation of many antioxidant enzymes that makes it capable of both decreasing deposition of Hg during co-exposure possibly due to the high affinity between Hg and Se and reducing the ROS level by activating antioxidant enzymes.The methodology involved chronic exposure of C. elegans to Phenylmercury Acetate (PMA), Se and co-exposure to PMA and Se on growth media. Assessing the oxidative damage following the exposure involved the probe; 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA). The total lipids of C. elegans (WT:N2) were extracted using chloroform and methanol in 2:1 ratio (v/v). Fatty acids composition of the extracted total lipids was converted to their corresponding fatty acids methyl esters (FAMEs) and analyzed by gas chromatography/accurate mass quadrupole time of flight mass spectrometry.The result showed that the exposure of C. elegans to PMA led to an increased generation of reactive oxygen species (ROS) that led to oxidative stress and the effect was dose dependent. Total fatty acids showed differences in percentage of individual fatty acids in all treatments and control experiment. Triacylglycerols (triglycerides) profile analyzed by Liquid Chromatography Quadrupole Time Of Flight (LCQTOF), changed by showing smaller or sometimes larger percentage in PMA treatment comparing to the non-treated control. It was evident that the antioxidant Se was able to counteract the PMA effect and bring the triacylglycerols' profiling back to normal.

ISBN: 9781392710180Subjects--Topical Terms:

677245
Environmental science.
Subjects--Index Terms:

Biomarker

Characterization of the Effects of Selenium on the Lipid Profile Induced by the Environmental Pollutant Phenylmercury Acetate Exposure in Caenorhabditis elegans.
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245 1 0 $a Characterization of the Effects of Selenium on the Lipid Profile Induced by the Environmental Pollutant Phenylmercury Acetate Exposure in Caenorhabditis elegans.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 90 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-08, Section: B.
500 $a Advisor: Abdel-Rahman, Fawzia.
502 $a Thesis (Ph.D.)--Texas Southern University, 2019.
506 $a This item must not be sold to any third party vendors.
520 $a The free-living nematode Caenorhabditis elegans is a proven model organism for lipid metabolism research. Experimental advantages of the C. elegans model include a fast- reproductive cycle, known cell lineages, and a fully sequenced genome. C. elegans studies showed that chronic exposure to mercury (Hg) compounds induces neuron degeneration likely due to the increase in reactive oxygen species (ROS). Selenium (Se) is an essential trace element required for activation of many antioxidant enzymes that makes it capable of both decreasing deposition of Hg during co-exposure possibly due to the high affinity between Hg and Se and reducing the ROS level by activating antioxidant enzymes.The methodology involved chronic exposure of C. elegans to Phenylmercury Acetate (PMA), Se and co-exposure to PMA and Se on growth media. Assessing the oxidative damage following the exposure involved the probe; 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA). The total lipids of C. elegans (WT:N2) were extracted using chloroform and methanol in 2:1 ratio (v/v). Fatty acids composition of the extracted total lipids was converted to their corresponding fatty acids methyl esters (FAMEs) and analyzed by gas chromatography/accurate mass quadrupole time of flight mass spectrometry.The result showed that the exposure of C. elegans to PMA led to an increased generation of reactive oxygen species (ROS) that led to oxidative stress and the effect was dose dependent. Total fatty acids showed differences in percentage of individual fatty acids in all treatments and control experiment. Triacylglycerols (triglycerides) profile analyzed by Liquid Chromatography Quadrupole Time Of Flight (LCQTOF), changed by showing smaller or sometimes larger percentage in PMA treatment comparing to the non-treated control. It was evident that the antioxidant Se was able to counteract the PMA effect and bring the triacylglycerols' profiling back to normal.
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653 $a Selenium
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