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Investigating the Use of Human Monoc...

Zhang, Xiaoqing.

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Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration./
作者:	Zhang, Xiaoqing.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:	252 p.
附註:	Source: Dissertations Abstracts International, Volume: 81-05, Section: B.
Contained By:	Dissertations Abstracts International81-05B.
標題:	Biomedical engineering. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=22587504
ISBN:	9781392732427

Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration.
Zhang, Xiaoqing.

Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 252 p.

Source: Dissertations Abstracts International, Volume: 81-05, Section: B.

Thesis (Ph.D.)--University of Toronto (Canada), 2019.

This item must not be sold to any third party vendors.

In vascular tissue engineering, it is desirable to promote the proliferation and uniform distribution of the tissue-specific cells within a biomaterial scaffold, and to stimulate the cells to synthesize and deposit appropriate extracellular matrix (ECM). The ability to generate and accumulate tissue-specific ECM is especially important since the ECM not only provides structural support for the engineered tissue, but also directs signalling towards surrounding cells in order to initiate remodelling processes. However, currently there is limited ability to promote ECM production during in vitro vascular tissue engineering. Aside from the limited ECM-promoting strategies, another challenge that has been recognized is the lack of a vascular smooth muscle cell (VSMC) source that is effective, robust and safe. The thesis project investigated in vitro VSMC-monocyte co-culture systems. It was found that achieving a desired release profile of growth factors and hydrolytic proteases to direct ECM-promoting and ECM-degrading activities in the multi-cellular microenvironment is effective at promoting ECM accumulation during vascular tissue engineering. Additionally, adipose derived stromal cells (ASCs) were differentiated into VSMC-like cells. ASC-VSMC-monocyte co-culture systems were constructed to track the potential phenotypic changes of ASC-VSMCs (in order to further assess the feasibility of applying ASC-VSMCs as a replacement cell source for mature tissue VSMCs). The study demonstrated that the degradable polar hydrophobic ionic polyurethane (D-PHI) biomaterial scaffold based ASC-VSMC-monocyte co-culture system showed more release of proinflammatory cytokines at week 1 and 2. However, it showed more anti-inflammatory/wound-healing cytokine release at week 4. This study revealed important signalling factors and some mechanisms underlying phenotypic switches of ASC-VSMCs exposed to monocytes during in vitro tissue engineering processes. This can provide insights into the feasibility of ASC-VSMCs for vascular tissue development. Additionally, this work improved understanding of the balance between inflammation and wound-healing processes during tissue regeneration and remodeling using biomaterial scaffolds.

ISBN: 9781392732427Subjects--Topical Terms:

535387
Biomedical engineering.
Subjects--Index Terms:

Vascular smooth muscle cells

Investigating the Use of Human Monocytes and Vascular Smooth Muscle-Like Cells Differentiated from Adipose Derived Stromal Cells for Vascular Tissue Regeneration.
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