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Epithelial Geometry Regulates Spindl...

Box, Kimberly Simone.

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Epithelial Geometry Regulates Spindle Orientation and Progenitor Fate During Formation of the Mammalian Epidermis.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Epithelial Geometry Regulates Spindle Orientation and Progenitor Fate During Formation of the Mammalian Epidermis./
Author:	Box, Kimberly Simone.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	94 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Contained By:	Dissertations Abstracts International81-04B.
Subject:	Molecular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13885241
ISBN:	9781085772594

Epithelial Geometry Regulates Spindle Orientation and Progenitor Fate During Formation of the Mammalian Epidermis.
Box, Kimberly Simone.

Epithelial Geometry Regulates Spindle Orientation and Progenitor Fate During Formation of the Mammalian Epidermis. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 94 p.

Source: Dissertations Abstracts International, Volume: 81-04, Section: B.

Thesis (Ph.D.)--Princeton University, 2019.

This item must not be sold to any third party vendors.

The ability to control cell fate through oriented division is imperative for the proper development of many organs, such as the stratified epidermis. Basal progenitor cells of the mammalian epidermis orient their divisions parallel or perpendicular to the basement membrane to expand the stem cell pool or produce differentiated stratified layers. Although any given basal cell is capable of either division orientation, the mechanisms that regulate the choice between division orientations are unknown. Here, using time-lapse imaging to follow divisions and fates of basal progenitors, we investigate the role of planar cell polarity (PCP) and tissue architecture during epidermal stratification. We find that embryos defective for the core PCP gene, Vangl2, exhibit increased perpendicular, asymmetric divisions at the expense of planar, symmetric divisions, but surprisingly, this is not due to defective Vangl2 function in the epidermis. We link this reduction in planar divisions to alterations in cell geometry and packing, which are indirectly caused by the neural tube closure defects characteristic of planar cell polarity mutants. We demonstrate that early in epidermal stratification, there is a close relationship between cell density, interphase cell height-to-width aspect ratio, and mitotic spindle orientation. These data show that basal epidermal cells utilize packing and shape, rather than cortical PCP cues, to inform division orientation. We propose a model in which local tissue architecture regulates the decision between symmetric and stratifying divisions, a mechanism that allows flexibility for basal stem cells to adapt to the needs of the developing tissue.

ISBN: 9781085772594Subjects--Topical Terms:

517296
Molecular biology.
Subjects--Index Terms:

Cell geometry

Epithelial Geometry Regulates Spindle Orientation and Progenitor Fate During Formation of the Mammalian Epidermis.
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245 1 0 $a Epithelial Geometry Regulates Spindle Orientation and Progenitor Fate During Formation of the Mammalian Epidermis.
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300 $a 94 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
500 $a Advisor: Devenport, Danelle.
502 $a Thesis (Ph.D.)--Princeton University, 2019.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a The ability to control cell fate through oriented division is imperative for the proper development of many organs, such as the stratified epidermis. Basal progenitor cells of the mammalian epidermis orient their divisions parallel or perpendicular to the basement membrane to expand the stem cell pool or produce differentiated stratified layers. Although any given basal cell is capable of either division orientation, the mechanisms that regulate the choice between division orientations are unknown. Here, using time-lapse imaging to follow divisions and fates of basal progenitors, we investigate the role of planar cell polarity (PCP) and tissue architecture during epidermal stratification. We find that embryos defective for the core PCP gene, Vangl2, exhibit increased perpendicular, asymmetric divisions at the expense of planar, symmetric divisions, but surprisingly, this is not due to defective Vangl2 function in the epidermis. We link this reduction in planar divisions to alterations in cell geometry and packing, which are indirectly caused by the neural tube closure defects characteristic of planar cell polarity mutants. We demonstrate that early in epidermal stratification, there is a close relationship between cell density, interphase cell height-to-width aspect ratio, and mitotic spindle orientation. These data show that basal epidermal cells utilize packing and shape, rather than cortical PCP cues, to inform division orientation. We propose a model in which local tissue architecture regulates the decision between symmetric and stratifying divisions, a mechanism that allows flexibility for basal stem cells to adapt to the needs of the developing tissue.
590 $a School code: 0181.
650 4 $a Molecular biology. $3 517296
650 4 $a Developmental biology. $3 592588
650 4 $a Cellular biology. $3 3172791
650 4 $a Physiology. $3 518431
650 4 $a Histology. $3 641250
650 4 $a Health sciences. $3 3168359
653 $a Cell geometry
653 $a Epidermis
653 $a Hertwig's Rule
653 $a Oriented cell division
653 $a Planar cell polarity
653 $a Stem cell
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690 $a 0758
690 $a 0379
690 $a 0566
690 $a 0414
690 $a 0719
710 2 $a Princeton University. $b Molecular Biology. $3 2101701
773 0 $t Dissertations Abstracts International $g 81-04B.
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791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13885241