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States and Fates of Primitive Neural...

Yammine, Samantha Z.

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States and Fates of Primitive Neural Stem Cells and Their Progeny in the Mammalian Brain.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	States and Fates of Primitive Neural Stem Cells and Their Progeny in the Mammalian Brain./
作者:	Yammine, Samantha Z.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:	187 p.
附註:	Source: Dissertations Abstracts International, Volume: 81-05, Section: B.
Contained By:	Dissertations Abstracts International81-05B.
標題:	Cellular biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13861520
ISBN:	9781392526934

States and Fates of Primitive Neural Stem Cells and Their Progeny in the Mammalian Brain.
Yammine, Samantha Z.

States and Fates of Primitive Neural Stem Cells and Their Progeny in the Mammalian Brain. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 187 p.

Source: Dissertations Abstracts International, Volume: 81-05, Section: B.

Thesis (Ph.D.)--University of Toronto (Canada), 2019.

This item must not be sold to any third party vendors.

The mature brain contains an incredible number and diversity of cells that are produced and maintained by heterogeneous pools of neural stem cells. Two distinct types of neural stem cells (NSCs) in the developing and adult mouse brain include primitive (p)NSCs and definitive (d)NSCs. PNSCs form clonogenic neurospheres when grown in LIF, arise ~E5.5, and persist into the adult brain. In this and other work we show adult pNSCs are very rare and quiescent, express Oct4, and give rise to GFAP+ definitive (d)NSCs that form neurospheres in EGF and FGF2. While pNSCs can serve as a reserve pool for an ablated dNSC population in the adult brain, characterization of this activation process remains of interest, and herein I propose this occurs through transition between unique molecular states.To better understand the functions of NSCs embryonically, we performed clonal lineage tracing within neurospheres grown in either LIF or EGF/FGF2, to enrich for neural progenitor cells (NPCs) directly downstream pNSCs or dNSCs, respectively. These clonal progenitor lineage tracing data allowed us to construct a hierarchy of progenitor subtypes downstream of pNSCs and dNSCs that were validated using single cell transcriptomics. Combined, these data provide single cell resolution of NPCs downstream of rare pNSCs that would likely be missed from population level analyses in vivo.

ISBN: 9781392526934Subjects--Topical Terms:

3172791
Cellular biology.
Subjects--Index Terms:

Development

States and Fates of Primitive Neural Stem Cells and Their Progeny in the Mammalian Brain.
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520 $a The mature brain contains an incredible number and diversity of cells that are produced and maintained by heterogeneous pools of neural stem cells. Two distinct types of neural stem cells (NSCs) in the developing and adult mouse brain include primitive (p)NSCs and definitive (d)NSCs. PNSCs form clonogenic neurospheres when grown in LIF, arise ~E5.5, and persist into the adult brain. In this and other work we show adult pNSCs are very rare and quiescent, express Oct4, and give rise to GFAP+ definitive (d)NSCs that form neurospheres in EGF and FGF2. While pNSCs can serve as a reserve pool for an ablated dNSC population in the adult brain, characterization of this activation process remains of interest, and herein I propose this occurs through transition between unique molecular states.To better understand the functions of NSCs embryonically, we performed clonal lineage tracing within neurospheres grown in either LIF or EGF/FGF2, to enrich for neural progenitor cells (NPCs) directly downstream pNSCs or dNSCs, respectively. These clonal progenitor lineage tracing data allowed us to construct a hierarchy of progenitor subtypes downstream of pNSCs and dNSCs that were validated using single cell transcriptomics. Combined, these data provide single cell resolution of NPCs downstream of rare pNSCs that would likely be missed from population level analyses in vivo.
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650 4 $a Biology. $3 522710
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653 $a Development
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653 $a Quiescence
653 $a Stem cells
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