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The Development of In Vivo Comprehen...

Liaghati Mobarhan, Yalda.

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The Development of In Vivo Comprehensive Multiphase NMR and Its Potential for the Investigation of Aquatic Toxicity.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The Development of In Vivo Comprehensive Multiphase NMR and Its Potential for the Investigation of Aquatic Toxicity./
作者:	Liaghati Mobarhan, Yalda.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
面頁冊數:	265 p.
附註:	Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
Contained By:	Dissertations Abstracts International81-04B.
標題:	Environmental science. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13808132
ISBN:	9781085776721

The Development of In Vivo Comprehensive Multiphase NMR and Its Potential for the Investigation of Aquatic Toxicity.
Liaghati Mobarhan, Yalda.

The Development of In Vivo Comprehensive Multiphase NMR and Its Potential for the Investigation of Aquatic Toxicity. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 265 p.

Source: Dissertations Abstracts International, Volume: 81-04, Section: B.

Thesis (Ph.D.)--University of Toronto (Canada), 2019.

This item must not be sold to any third party vendors.

NMR based metabolomics has been extensively used to provide a molecular-level understanding of underlying biological processes due to environmental stimuli. Previously reported in vivo NMR studies were limited to detecting dynamic and soluble entities, resulting in the need for an approach to detect all bonds (solids, gels and liquids in vivo) and thus provide a comprehensive overview as to all components inside a living organism. CMP-NMR integrates all the electronics from HR-MAS (gel-phase NMR), with high power RF handling to study rigid solids, into a single probe. Therefore, in vivo CMP-NMR holds potential to correlate rapid stress responses (metabolite fluxes) to slower structural changes (thinning of shells). In this regard, the focus of this dissertation was the development of CMP-NMR for the study of living systems. In Chapter 2, CMP-NMR combined with spectral editing isolates the signals arising from the rigid solids (chitin), to gel (lipid membranes) and liquids (amino acids) in Hyalella azteca into discrete spectra for each sub-component. Additionally, 13C isotopic enrichment and 2D NMR enabled the assignment of nearly 40 metabolites. However, the stress exerted on the organisms due to the fast spinning (2.5 KHz) remained a major challenge of the approach. To address this, Chapter 3 introduces a slow spinning technique by concatenating PURGE and TOSS.243, which leads to high resolution in vivo 1D 1H NMR profiles down to 300 Hz. In Chapter 4 hetero-nuclear (31P, 19F 2H, 15N, NMR) experiments are introduced to follow the fate of the contaminants inside the living organism and 2D correlations (1H-13C, 2H-13C) monitor the biomolecular responses of the organism to the nutritional substrates. In Chapter 5 2H/13C isotopic enrichment of the organisms, is combined with 2D 2H-13C NMR leading to the first high resolution sideband free spectra at 50 Hz and extending the experimental time up to 48 h. Combined in vivo CMP NMR techniques show great potential for future application in environmental toxicity risk assessments.

ISBN: 9781085776721Subjects--Topical Terms:

677245
Environmental science.
Subjects--Index Terms:

Bulk magnetization

The Development of In Vivo Comprehensive Multiphase NMR and Its Potential for the Investigation of Aquatic Toxicity.
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