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Novel Approaches of Manipulating the...

Aljasim, Kawther Sahib.

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Novel Approaches of Manipulating the FT3 Early Flowering Gene to Induce Early Flowering and Fruiting in Juvenile Citrus Hybrids and Transgenic Plants.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Novel Approaches of Manipulating the FT3 Early Flowering Gene to Induce Early Flowering and Fruiting in Juvenile Citrus Hybrids and Transgenic Plants./
Author:	Aljasim, Kawther Sahib.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	257 p.
Notes:	Source: Dissertations Abstracts International, Volume: 82-04, Section: B.
Contained By:	Dissertations Abstracts International82-04B.
Subject:	Horticulture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27833735
ISBN:	9798684604027

Novel Approaches of Manipulating the FT3 Early Flowering Gene to Induce Early Flowering and Fruiting in Juvenile Citrus Hybrids and Transgenic Plants.
Aljasim, Kawther Sahib.

Novel Approaches of Manipulating the FT3 Early Flowering Gene to Induce Early Flowering and Fruiting in Juvenile Citrus Hybrids and Transgenic Plants. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 257 p.

Source: Dissertations Abstracts International, Volume: 82-04, Section: B.

Thesis (Ph.D.)--University of Florida, 2020.

This item must not be sold to any third party vendors.

The juvenility period in citrus is generally long requiring 5-22 years to achieve flowering and fruit set. The FT protein plays a major role in controlling the flowering, and mobility throughout the plant. A transgenic Carrizo citrange overexpresses the FLOWERING LOCUS T (FT) gene, resulting in accelerated flowering and fruiting within 18 months. These transgenic plants were propagated using two different methods: First method: micropropagation used Murashige and Tucker (MT) and Murashige and Skoog (MS) basal media supplemented with different concentrations of 6 - Benzyl adenine (BA) and malt extract for optimal shoot multiplication. Carrizo lines rooted in vitro using MS medium supplemented with three different concentrations of NAA (0.02, 0.03, and 0.04 mg L-1). Rooting stages were made ex Vitro using Jiffy 7 compressed peat moss pellets and compared with in vitro rooting using MS medium supplemented with 0.02 mg L-1 NAA. The acclimatization stage for Carrizo lines was made under three different environmental conditions. A second propagation method was also tested: rooted cuttings testing different auxin resources to achieve optimal rooting.To determine if the early flowering trait was transmittable through a graft union to juvenile scions, selected juvenile scions were grafted on the transgenic Carrizo. Most of the juvenile scions then quickly flowered. This suggests a successful movement of the FT protein through the graft union into the scion, initiating early flowering. However, after the initial bloom, sustained flowering in the grafted scions was not observed, although it continued in the ungrafted transgenic Carrizo rootstocks.Citrus Tristeza Virus vector was developed to introduce and cause expression of foreign genes in citrus, achieving genes expression months after plant infection. In this project, a modified CTV vector carrying the FT gene was used to infect juvenile citrus disease-resistant plants (cybrids and sweet orange lines) that were developed by the UF/CREC citrus breeding program. Confirmation of CTV-FT3 stability in the infected plants was made based on the PCR results. The positive CTV-FT3 plants are expected to be flowering and fruiting in the near future.

ISBN: 9798684604027Subjects--Topical Terms:

555447
Horticulture.
Subjects--Index Terms:

Carrizo citrange

Novel Approaches of Manipulating the FT3 Early Flowering Gene to Induce Early Flowering and Fruiting in Juvenile Citrus Hybrids and Transgenic Plants.
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500 $a Source: Dissertations Abstracts International, Volume: 82-04, Section: B.
500 $a Advisor: Grosser, Jude.
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520 $a The juvenility period in citrus is generally long requiring 5-22 years to achieve flowering and fruit set. The FT protein plays a major role in controlling the flowering, and mobility throughout the plant. A transgenic Carrizo citrange overexpresses the FLOWERING LOCUS T (FT) gene, resulting in accelerated flowering and fruiting within 18 months. These transgenic plants were propagated using two different methods: First method: micropropagation used Murashige and Tucker (MT) and Murashige and Skoog (MS) basal media supplemented with different concentrations of 6 - Benzyl adenine (BA) and malt extract for optimal shoot multiplication. Carrizo lines rooted in vitro using MS medium supplemented with three different concentrations of NAA (0.02, 0.03, and 0.04 mg L-1). Rooting stages were made ex Vitro using Jiffy 7 compressed peat moss pellets and compared with in vitro rooting using MS medium supplemented with 0.02 mg L-1 NAA. The acclimatization stage for Carrizo lines was made under three different environmental conditions. A second propagation method was also tested: rooted cuttings testing different auxin resources to achieve optimal rooting.To determine if the early flowering trait was transmittable through a graft union to juvenile scions, selected juvenile scions were grafted on the transgenic Carrizo. Most of the juvenile scions then quickly flowered. This suggests a successful movement of the FT protein through the graft union into the scion, initiating early flowering. However, after the initial bloom, sustained flowering in the grafted scions was not observed, although it continued in the ungrafted transgenic Carrizo rootstocks.Citrus Tristeza Virus vector was developed to introduce and cause expression of foreign genes in citrus, achieving genes expression months after plant infection. In this project, a modified CTV vector carrying the FT gene was used to infect juvenile citrus disease-resistant plants (cybrids and sweet orange lines) that were developed by the UF/CREC citrus breeding program. Confirmation of CTV-FT3 stability in the infected plants was made based on the PCR results. The positive CTV-FT3 plants are expected to be flowering and fruiting in the near future.
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650 4 $a Citrus fruits. $3 1000206
653 $a Carrizo citrange
653 $a Citrus
653 $a Citrus Tristeza Virus viral vector
653 $a Early flowering
653 $a FLOWERING LOCUS T FT gene
653 $a Tissue culture
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710 2 $a University of Florida. $b Horticultural Science. $3 3549203
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