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Detection of Small Molecules Using G...

Narayanan, Manojkumar.

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Detection of Small Molecules Using Genetically Encoded Nucleic Acid Circuits.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Detection of Small Molecules Using Genetically Encoded Nucleic Acid Circuits./
Author:	Narayanan, Manojkumar.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2020,
Description:	81 p.
Notes:	Source: Masters Abstracts International, Volume: 81-12.
Contained By:	Masters Abstracts International81-12.
Subject:	Biochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=27833733
ISBN:	9798645471989

Detection of Small Molecules Using Genetically Encoded Nucleic Acid Circuits.
Narayanan, Manojkumar.

Detection of Small Molecules Using Genetically Encoded Nucleic Acid Circuits. - Ann Arbor : ProQuest Dissertations & Theses, 2020 - 81 p.

Source: Masters Abstracts International, Volume: 81-12.

Thesis (M.S.)--University of Massachusetts Lowell, 2020.

This item must not be sold to any third party vendors.

Detecting intracellular levels of small molecules in live cells is of prime importance in understanding the functions of the cell. Small molecules include cellular metabolites, signaling molecules and drugs that act as effectors, thus altering the overall function of the cell. Genetically encoded biosensors have been used to detect these small molecules in live cells.Enzyme-free genetically encoded nucleic acid circuits such as Catalytic Hairpin Assembly (CHA) and Hybridization Chain Reaction (HCR) have been used in bioanalysis. CHA and HCR are RNA-based circuits that amplify output signals, thus enabling sensitive detection. These two circuits have been used so far for detection of low-abundance RNA in cells. Small molecule detection has been of quite a challenge owing to their low abundance.Herein, we have reported genetically encoded RNA-based Hybridization Chain Reaction circuit to detect cellular levels of Theophylline. Theophylline is a small molecule drug used as bronchodilator for the treatment of various respiratory diseases. A Theophylline Aptamer-Hammerhead Ribozyme binds the molecule and undergoes a self-cleavage, which releases the fluorogenic RNA aptamer, Broccoli. The split version of Broccoli is used as a reporter. In HCR, one molecule of target can trigger a chain reaction of hybridization of broccoli, thus producing an enhanced signal for small abundance. We show that this method can be employed to design similar sensors to detect various other biomolecules.

ISBN: 9798645471989Subjects--Topical Terms:

518028
Biochemistry.
Subjects--Index Terms:

Aptamers

Detection of Small Molecules Using Genetically Encoded Nucleic Acid Circuits.
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245 1 0 $a Detection of Small Molecules Using Genetically Encoded Nucleic Acid Circuits.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2020
300 $a 81 p.
500 $a Source: Masters Abstracts International, Volume: 81-12.
500 $a Advisor: You, Mingxu.
502 $a Thesis (M.S.)--University of Massachusetts Lowell, 2020.
506 $a This item must not be sold to any third party vendors.
520 $a Detecting intracellular levels of small molecules in live cells is of prime importance in understanding the functions of the cell. Small molecules include cellular metabolites, signaling molecules and drugs that act as effectors, thus altering the overall function of the cell. Genetically encoded biosensors have been used to detect these small molecules in live cells.Enzyme-free genetically encoded nucleic acid circuits such as Catalytic Hairpin Assembly (CHA) and Hybridization Chain Reaction (HCR) have been used in bioanalysis. CHA and HCR are RNA-based circuits that amplify output signals, thus enabling sensitive detection. These two circuits have been used so far for detection of low-abundance RNA in cells. Small molecule detection has been of quite a challenge owing to their low abundance.Herein, we have reported genetically encoded RNA-based Hybridization Chain Reaction circuit to detect cellular levels of Theophylline. Theophylline is a small molecule drug used as bronchodilator for the treatment of various respiratory diseases. A Theophylline Aptamer-Hammerhead Ribozyme binds the molecule and undergoes a self-cleavage, which releases the fluorogenic RNA aptamer, Broccoli. The split version of Broccoli is used as a reporter. In HCR, one molecule of target can trigger a chain reaction of hybridization of broccoli, thus producing an enhanced signal for small abundance. We show that this method can be employed to design similar sensors to detect various other biomolecules.
590 $a School code: 0111.
650 4 $a Biochemistry. $3 518028
650 4 $a Nanotechnology. $3 526235
650 4 $a Analytical chemistry. $3 3168300
653 $a Aptamers
653 $a Broccoli
653 $a Catalytic hairpin assembly
653 $a Genetically encoded biosensors
653 $a Hybridization chain reaction
653 $a Theophylline
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690 $a 0652
690 $a 0486
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793 $a English
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