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Cytotoxic T lymphocyte responses to ...

Grimsrud, Carrie Justine.

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Cytotoxic T lymphocyte responses to Marek's disease herpesvirus -encoded glycoproteins and their impairment by chicken infectious anemia virus.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Cytotoxic T lymphocyte responses to Marek's disease herpesvirus -encoded glycoproteins and their impairment by chicken infectious anemia virus./
Author:	Grimsrud, Carrie Justine.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2002,
Description:	221 p.
Notes:	Source: Dissertations Abstracts International, Volume: 64-02, Section: B.
Contained By:	Dissertations Abstracts International64-02B.
Subject:	Immunology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3050395
ISBN:	9780493660011

Cytotoxic T lymphocyte responses to Marek's disease herpesvirus -encoded glycoproteins and their impairment by chicken infectious anemia virus.
Grimsrud, Carrie Justine.

Cytotoxic T lymphocyte responses to Marek's disease herpesvirus -encoded glycoproteins and their impairment by chicken infectious anemia virus. - Ann Arbor : ProQuest Dissertations & Theses, 2002 - 221 p.

Source: Dissertations Abstracts International, Volume: 64-02, Section: B.

Thesis (Ph.D.)--Cornell University, 2002.

This item must not be sold to any third party vendors.

Marek's disease (MD), a lymphoma in chickens, is caused by MD herpesvirus (MDV). Cell-mediated immunity (CMI) is important for protection to MD. Reticuloendotheliosis (REV)-transformed lymphoblastoid cell lines stably transfected with various MDV genes have been used to identify MDV-encoded proteins recognized by cytotoxic T lymphocyte (CTL) responses. Cell lines derived from MDV resistant (N2a) and susceptible (P2a) chicken lines with defined major histocompatibility complex (MHC) haplotypes expressing MDV glycoprotein genes gC, gD, gE, gH, gl, gK, gL, and gM were developed. These cell lines were used as target cells in chromium release assays (CRA) using MDV- or REV-sensitized splenocytes as effector cells to examine antigen-specific CTL responses. Glycoprotein I was recognized by CTL in both the N2a and P2a line, while gC, gH, gK, and gL were only recognized by CTL from the resistant N2a line. Glycoprotein E was recognized by P2a CTL only, and gD and gM were not consistently recognized by CTL from either strain. An outbreak of chicken infectious anemia virus (CIAV) in the specific-pathogen-free departmental flocks during the course of these experiments led to the discovery that REV, and MDV-specific CTL responses were impaired during concomitant CIAV infection in older birds lacking maternal antibodies. Natural killer cell activity was not impaired in CIAV-exposed chickens over a six-week time period. TagMan™ real-time quantitative PCR and RT-PCR assays were developed to determine CIAV DNA and transcript levels. These assays were used to examine relationships between viral load, maternal antibody status, and CTL responses in chickens infected with REV and/or CIAV. Increased CIAV viral load and transcription correlated with decreased REV-specific CTL activity in chicks lacking maternal antibodies. In contrast, maternal antibody-positive chickens generated REV-specific CTL. The identification of epitopes recognized by CTL is important for the development of recombinant vaccines. To facilitate the identification of epitopes a targeting vector was constructed to generate TAP-deficient cell lines unable to present self-peptides in an MHC class I context. Several cell lines were selected after stable transfection with the construct and will be tested in the near future for the ability to present exogenous peptides to MDV-specific CTL.

ISBN: 9780493660011Subjects--Topical Terms:

611031
Immunology.
Subjects--Index Terms:

Chicken infectious anemia virus

Cytotoxic T lymphocyte responses to Marek's disease herpesvirus -encoded glycoproteins and their impairment by chicken infectious anemia virus.
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520 $a Marek's disease (MD), a lymphoma in chickens, is caused by MD herpesvirus (MDV). Cell-mediated immunity (CMI) is important for protection to MD. Reticuloendotheliosis (REV)-transformed lymphoblastoid cell lines stably transfected with various MDV genes have been used to identify MDV-encoded proteins recognized by cytotoxic T lymphocyte (CTL) responses. Cell lines derived from MDV resistant (N2a) and susceptible (P2a) chicken lines with defined major histocompatibility complex (MHC) haplotypes expressing MDV glycoprotein genes gC, gD, gE, gH, gl, gK, gL, and gM were developed. These cell lines were used as target cells in chromium release assays (CRA) using MDV- or REV-sensitized splenocytes as effector cells to examine antigen-specific CTL responses. Glycoprotein I was recognized by CTL in both the N2a and P2a line, while gC, gH, gK, and gL were only recognized by CTL from the resistant N2a line. Glycoprotein E was recognized by P2a CTL only, and gD and gM were not consistently recognized by CTL from either strain. An outbreak of chicken infectious anemia virus (CIAV) in the specific-pathogen-free departmental flocks during the course of these experiments led to the discovery that REV, and MDV-specific CTL responses were impaired during concomitant CIAV infection in older birds lacking maternal antibodies. Natural killer cell activity was not impaired in CIAV-exposed chickens over a six-week time period. TagMan™ real-time quantitative PCR and RT-PCR assays were developed to determine CIAV DNA and transcript levels. These assays were used to examine relationships between viral load, maternal antibody status, and CTL responses in chickens infected with REV and/or CIAV. Increased CIAV viral load and transcription correlated with decreased REV-specific CTL activity in chicks lacking maternal antibodies. In contrast, maternal antibody-positive chickens generated REV-specific CTL. The identification of epitopes recognized by CTL is important for the development of recombinant vaccines. To facilitate the identification of epitopes a targeting vector was constructed to generate TAP-deficient cell lines unable to present self-peptides in an MHC class I context. Several cell lines were selected after stable transfection with the construct and will be tested in the near future for the ability to present exogenous peptides to MDV-specific CTL.
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