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Effect of Peptides' Binding on the A...

Kaisersberger Vincek, Maja.

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Effect of Peptides' Binding on the Antimicrobial Activity and Biocompatibility of Protein-Based Substrates.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Effect of Peptides' Binding on the Antimicrobial Activity and Biocompatibility of Protein-Based Substrates./
作者:	Kaisersberger Vincek, Maja.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2017,
面頁冊數:	137 p.
附註:	Source: Dissertations Abstracts International, Volume: 79-04, Section: B.
Contained By:	Dissertations Abstracts International79-04B.
標題:	Polymer chemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10617256
ISBN:	9780355206838

Effect of Peptides' Binding on the Antimicrobial Activity and Biocompatibility of Protein-Based Substrates.
Kaisersberger Vincek, Maja.

Effect of Peptides' Binding on the Antimicrobial Activity and Biocompatibility of Protein-Based Substrates. - Ann Arbor : ProQuest Dissertations & Theses, 2017 - 137 p.

Source: Dissertations Abstracts International, Volume: 79-04, Section: B.

Thesis (Math.Sc.D.)--Univerza v Mariboru (Slovenia), 2017.

This item must not be sold to any third party vendors.

This work reveals the effect of coupling approach (chemical by using carbodiimide chemistry and grafting-to vs. grafting-from synthesis routes, and enzymatic by using transglutaminase) of a hydrophilic ϵ-poly-L-lysine (ϵPL) and an amphiphilic oligo-acyl-lysyl (OAK) derivative (K-7α 12-OH) to wool fibers and gelatine (GEL) macromolecules, respectively, and substrates antibacterial activity against Gram-negative E. coli and Gram-positive S. aureus bacteria after 1-24 h of exposure, as well as their cytotoxicity. Different spectroscopic (ultraviolet-visible, infrared, fluorescence and electron paramagnetic resonance) and separation techniques (size-exclusion chromatography and capillary zone electrophoresis) as well as zeta potential and potentiometric titration analysis, were performed to confirm the covalent coupling of ϵPL/OAK, and to determine the amount and orientation of its immobilisation. The highest and kinetically the fastest level of bacterial reduction was achieved with wool/GEL functionalised with ϵPL/OAK by chemical grafting-to approach. This effect correlated with both the highest grafting yield and conformationally the highly-flexible (brush-like) orientation linkage of ϵPL/OAK, implicating on the highest amount of accessible amino groups interacting with bacterial membrane. However, OAK's amphipathic structure, the cationic charge and the hydrophobic moieties, resulted to relatively high reduction of S. aureus for grafting-from and the enzymatic coupling approaches using OAK-functionalised GEL. The ϵPL/OAK-functionalised GEL did not induce toxicity in human osteoblast cells, even at ~25-fold higher concentration than bacterial minimum inhibitory (MIC) concentration of ϵPL/OAK, supporting their potential usage in biomedical applications. It was also shown that non-ionic surfactant adsorbs strongly onto the wool surface during the process of washing, thereby blocking the functional sites of immobilized ϵPL and decreases its antibacterial efficiency.

ISBN: 9780355206838Subjects--Topical Terms:

3173488
Polymer chemistry.
Subjects--Index Terms:

Antimicrobial peptides‎

Effect of Peptides' Binding on the Antimicrobial Activity and Biocompatibility of Protein-Based Substrates.
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