東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Single-Nanoparticle Microscopy of DN...

Matthews, Brandon Marcellus.

Linked to FindBook

Google Book

Amazon

博客來

Single-Nanoparticle Microscopy of DNA-Incorporated Hydrogel Nanoparticles.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Single-Nanoparticle Microscopy of DNA-Incorporated Hydrogel Nanoparticles./
Author:	Matthews, Brandon Marcellus.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	89 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-03, Section: B.
Contained By:	Dissertations Abstracts International81-03B.
Subject:	Physical chemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13895506
ISBN:	9781085608022

Single-Nanoparticle Microscopy of DNA-Incorporated Hydrogel Nanoparticles.
Matthews, Brandon Marcellus.

Single-Nanoparticle Microscopy of DNA-Incorporated Hydrogel Nanoparticles. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 89 p.

Source: Dissertations Abstracts International, Volume: 81-03, Section: B.

Thesis (Ph.D.)--University of California, Irvine, 2019.

This item must not be sold to any third party vendors.

This dissertation is the cumulative work towards the development of bioactive hydrogel nanoparticles by way of DNA incorporation. First, a collection of orthogonal DNA sequences is fabricated and binding coefficients quantified via surface plasmon resonance imaging (SPRI). Then, using a sample sequence from the collection, the DNA is polymerized into the backbone of hydrogel nanoparticles (HNPs) to be characterized by single-nanoparticle SPRI microscopy as surface adsorption measurements to gold thin-films. The SPRI responses, Δ%RNP, from several hundred nanoparticle adsorption events are used to generate frequency distribution histograms that characterize the nanoparticle size, composition, and bioactivity through complementary DNA hybridization and enzymatic-induced hydrolysis. These analyses reveal a dependence on the base sequence to aid in the incorporation of DNA into the HNPs. Lastly, DNA incorporation is further elaborated upon by the formation of nanocomposite HNPs, wherein the magnetic nanoparticle ferrite is present during DNA-HNP formation, alluding to a preferential covalent incorporation of DNA over that of ferrite nanoparticles as characterized by SPRI microscopy.

ISBN: 9781085608022Subjects--Topical Terms:

1981412
Physical chemistry.
Subjects--Index Terms:

Single-nanoparticle microscopy

Single-Nanoparticle Microscopy of DNA-Incorporated Hydrogel Nanoparticles.
LDR:02384nmm a2200349 4500 001 2267609
005 20200724103013.5
008 220629s2019 ||||||||||||||||| ||eng d
020 $a 9781085608022
035 $a (MiAaPQ)AAI13895506
035 $a AAI13895506
040 $a MiAaPQ $c MiAaPQ
100 1 $a Matthews, Brandon Marcellus. $3 3544866
245 1 0 $a Single-Nanoparticle Microscopy of DNA-Incorporated Hydrogel Nanoparticles.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 89 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-03, Section: B.
500 $a Advisor: Corn, Robert M.
502 $a Thesis (Ph.D.)--University of California, Irvine, 2019.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a This dissertation is the cumulative work towards the development of bioactive hydrogel nanoparticles by way of DNA incorporation. First, a collection of orthogonal DNA sequences is fabricated and binding coefficients quantified via surface plasmon resonance imaging (SPRI). Then, using a sample sequence from the collection, the DNA is polymerized into the backbone of hydrogel nanoparticles (HNPs) to be characterized by single-nanoparticle SPRI microscopy as surface adsorption measurements to gold thin-films. The SPRI responses, Δ%RNP, from several hundred nanoparticle adsorption events are used to generate frequency distribution histograms that characterize the nanoparticle size, composition, and bioactivity through complementary DNA hybridization and enzymatic-induced hydrolysis. These analyses reveal a dependence on the base sequence to aid in the incorporation of DNA into the HNPs. Lastly, DNA incorporation is further elaborated upon by the formation of nanocomposite HNPs, wherein the magnetic nanoparticle ferrite is present during DNA-HNP formation, alluding to a preferential covalent incorporation of DNA over that of ferrite nanoparticles as characterized by SPRI microscopy.
590 $a School code: 0030.
650 4 $a Physical chemistry. $3 1981412
650 4 $a Analytical chemistry. $3 3168300
650 4 $a Chemistry. $3 516420
653 $a Single-nanoparticle microscopy
653 $a DNA-incorporated hydrogel nanoparticles
690 $a 0494
690 $a 0486
690 $a 0485
710 2 $a University of California, Irvine. $b Chemistry - Ph.D.. $3 2094938
773 0 $t Dissertations Abstracts International $g 81-03B.
790 $a 0030
791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13895506