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Epigenetic Reprogramming, Apoptosis,...

Moley, Laura A.

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Epigenetic Reprogramming, Apoptosis, and Developmental Competence in Cloned Embryos.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Epigenetic Reprogramming, Apoptosis, and Developmental Competence in Cloned Embryos./
Author:	Moley, Laura A.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	285 p.
Notes:	Source: Dissertations Abstracts International, Volume: 81-05, Section: B.
Contained By:	Dissertations Abstracts International81-05B.
Subject:	Animal sciences. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=22587189
ISBN:	9781088351949

Epigenetic Reprogramming, Apoptosis, and Developmental Competence in Cloned Embryos.
Moley, Laura A.

Epigenetic Reprogramming, Apoptosis, and Developmental Competence in Cloned Embryos. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 285 p.

Source: Dissertations Abstracts International, Volume: 81-05, Section: B.

Thesis (Ph.D.)--Utah State University, 2019.

This item must not be sold to any third party vendors.

Cloning through somatic cell nuclear transfer (SCNT) remains highly inefficient twenty years after the first demonstration of the technology with the birth of Dolly. By increasing efficiency by selecting the embryos early in development that are most likely to succeed following transfer into a surrogate mother, the technology could be more routinely utilized to enhance animal agriculture production. SCNT is believed to be highly inefficient as a result of incorrect DNA methylation and gene expression that are accumulated because of the SCNT technique. We proposed the use of a non-toxic, non-invasive detector of cell death, to quantitatively assess embryo competency prior to embryo transfer. We believed we could use SR-FLICA to identify the embryos with low levels of cell death as a result of proper DNA methylation and gene expression. By analyzing the whole embryo, differences in gene expression and DNA methylation were identified in embryos with high and low levels of cell death. However, the level of cell death did not prove to be a reliable indicator of embryo quality in predicting pregnancy outcome. This data supports the commonly held hypothesis that DNA methylation and gene expression after SCNT have random defects as a result of the random nature of resetting the DNA for embryo development. More research is required to identify the embryos which will prove to be successful following SCNT and embryo transfer.

ISBN: 9781088351949Subjects--Topical Terms:

3174829
Animal sciences.

Epigenetic Reprogramming, Apoptosis, and Developmental Competence in Cloned Embryos.
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100 1 $a Moley, Laura A. $3 3540356
245 1 0 $a Epigenetic Reprogramming, Apoptosis, and Developmental Competence in Cloned Embryos.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 285 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-05, Section: B.
500 $a Advisor: Isom, S Clay.
502 $a Thesis (Ph.D.)--Utah State University, 2019.
506 $a This item must not be sold to any third party vendors.
520 $a Cloning through somatic cell nuclear transfer (SCNT) remains highly inefficient twenty years after the first demonstration of the technology with the birth of Dolly. By increasing efficiency by selecting the embryos early in development that are most likely to succeed following transfer into a surrogate mother, the technology could be more routinely utilized to enhance animal agriculture production. SCNT is believed to be highly inefficient as a result of incorrect DNA methylation and gene expression that are accumulated because of the SCNT technique. We proposed the use of a non-toxic, non-invasive detector of cell death, to quantitatively assess embryo competency prior to embryo transfer. We believed we could use SR-FLICA to identify the embryos with low levels of cell death as a result of proper DNA methylation and gene expression. By analyzing the whole embryo, differences in gene expression and DNA methylation were identified in embryos with high and low levels of cell death. However, the level of cell death did not prove to be a reliable indicator of embryo quality in predicting pregnancy outcome. This data supports the commonly held hypothesis that DNA methylation and gene expression after SCNT have random defects as a result of the random nature of resetting the DNA for embryo development. More research is required to identify the embryos which will prove to be successful following SCNT and embryo transfer.
590 $a School code: 0241.
650 4 $a Animal sciences. $3 3174829
650 4 $a Veterinary medicine. $3 642774
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710 2 $a Utah State University. $b Animal, Dairy and Veterinary Sciences. $3 3194303
773 0 $t Dissertations Abstracts International $g 81-05B.
790 $a 0241
791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=22587189