東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Methods for fragments screening usin...

Gosu, Ramachandraiah.

FindBook

Google Book

Amazon

博客來

Methods for fragments screening using surface plasmon resonance

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Methods for fragments screening using surface plasmon resonance/ edited by Sameer Mahmood Zaheer, Ramachandraiah Gosu.
其他作者:	Gosu, Ramachandraiah.
出版者:	Singapore :Springer Singapore : : 2021.,
面頁冊數:	xvi, 113 p. :ill. (some col.), digital ;24 cm.
內容註:	Chapter 1. Introduction to Surface Plasmon Resonance -- Chapter 2. Principle of Surface Plasmon Resonance (OneStep) -- Chapter 3. Preparation of protein with Histidine Tag for Amine coupling -- Chapter 4. Preparation of protein with Avidin Tag for Biotin-based Capture -- Chapter 5. Protein stability using Thermal Shift Assay (TSA) - pH Tolerance -- Chapter 6. Protein stability using Thermal Shift Assay (TSA) - DMSO Tolerance -- Chapter 7. Selection and Identification of Fragment library -- Chapter 8. Preparation of Sensor -- Chapter 9. Optimization and validation of Amine coupling of Ligand -- Chapter 10. Optimization and Validation of Strepavidin/Biotin-based capture of Ligand -- Chapter 11. Preparation of Fragments for Screening in SPR -- Chapter 12. Screening of Fragments in SPR -- Chapter 13. Identification of Fragment Hits -- Chapter 14. Data Analysis and Confirmation of Hits.
Contained By:	Springer Nature eBook
標題:	Proteins - Analysis. -
電子資源:	https://doi.org/10.1007/978-981-16-1536-8
ISBN:	9789811615368

Methods for fragments screening using surface plasmon resonance
Methods for fragments screening using surface plasmon resonance[electronic resource] /edited by Sameer Mahmood Zaheer, Ramachandraiah Gosu. - Singapore :Springer Singapore :2021. - xvi, 113 p. :ill. (some col.), digital ;24 cm.

Chapter 1. Introduction to Surface Plasmon Resonance -- Chapter 2. Principle of Surface Plasmon Resonance (OneStep) -- Chapter 3. Preparation of protein with Histidine Tag for Amine coupling -- Chapter 4. Preparation of protein with Avidin Tag for Biotin-based Capture -- Chapter 5. Protein stability using Thermal Shift Assay (TSA) - pH Tolerance -- Chapter 6. Protein stability using Thermal Shift Assay (TSA) - DMSO Tolerance -- Chapter 7. Selection and Identification of Fragment library -- Chapter 8. Preparation of Sensor -- Chapter 9. Optimization and validation of Amine coupling of Ligand -- Chapter 10. Optimization and Validation of Strepavidin/Biotin-based capture of Ligand -- Chapter 11. Preparation of Fragments for Screening in SPR -- Chapter 12. Screening of Fragments in SPR -- Chapter 13. Identification of Fragment Hits -- Chapter 14. Data Analysis and Confirmation of Hits.

This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR) The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE) It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.

ISBN: 9789811615368

Standard No.: 10.1007/978-981-16-1536-8doiSubjects--Topical Terms:

621010
Proteins
--Analysis.

LC Class. No.: QP551.5 / .M48 2021

Dewey Class. No.: 572.636

Methods for fragments screening using surface plasmon resonance
LDR:02983nmm a2200337 a 4500 001 2251047
003 DE-He213
005 20210915143303.0
006 m d
007 cr nn 008maaau
008 220215s2021 si s 0 eng d
020 $a 9789811615368 $q (electronic bk.)
020 $a 9789811615351 $q (paper)
024 7 $a 10.1007/978-981-16-1536-8 $2 doi
035 $a 978-981-16-1536-8
040 $a GP $c GP
041 0 $a eng
050 4 $a QP551.5 $b .M48 2021
072 7 $a PS $2 bicssc
072 7 $a SCI008000 $2 bisacsh
072 7 $a PS $2 thema
072 7 $a PDN $2 thema
082 0 4 $a 572.636 $2 23
090 $a QP551.5 $b .M592 2021
245 0 0 $a Methods for fragments screening using surface plasmon resonance $h [electronic resource] / $c edited by Sameer Mahmood Zaheer, Ramachandraiah Gosu.
260 $a Singapore : $b Springer Singapore : $b Imprint: Springer, $c 2021.
300 $a xvi, 113 p. : $b ill. (some col.), digital ; $c 24 cm.
505 0 $a Chapter 1. Introduction to Surface Plasmon Resonance -- Chapter 2. Principle of Surface Plasmon Resonance (OneStep) -- Chapter 3. Preparation of protein with Histidine Tag for Amine coupling -- Chapter 4. Preparation of protein with Avidin Tag for Biotin-based Capture -- Chapter 5. Protein stability using Thermal Shift Assay (TSA) - pH Tolerance -- Chapter 6. Protein stability using Thermal Shift Assay (TSA) - DMSO Tolerance -- Chapter 7. Selection and Identification of Fragment library -- Chapter 8. Preparation of Sensor -- Chapter 9. Optimization and validation of Amine coupling of Ligand -- Chapter 10. Optimization and Validation of Strepavidin/Biotin-based capture of Ligand -- Chapter 11. Preparation of Fragments for Screening in SPR -- Chapter 12. Screening of Fragments in SPR -- Chapter 13. Identification of Fragment Hits -- Chapter 14. Data Analysis and Confirmation of Hits.
520 $a This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR) The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE) It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.
650 0 $a Proteins $x Analysis. $3 621010
650 0 $a Protein-protein interactions. $3 767666
650 0 $a Surface plasmon resonance. $3 1068658
650 1 4 $a Biological Techniques. $3 900035
650 2 4 $a Chemistry/Food Science, general. $3 894122
650 2 4 $a Protein Science. $3 1067276
650 2 4 $a Pharmacology/Toxicology. $3 890953
700 1 $a Gosu, Ramachandraiah. $3 3517664
700 1 $a Zaheer, Sameer Mahmood. $3 3517663
710 2 $a SpringerLink (Online service) $3 836513
773 0 $t Springer Nature eBook
856 4 0 $u https://doi.org/10.1007/978-981-16-1536-8
950 $a Biomedical and Life Sciences (SpringerNature-11642)