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The Role of the Vg1 Zebrafish Orthol...

Pelliccia, Jose Luis.

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The Role of the Vg1 Zebrafish Ortholog GDF3 in Nodal-Dependent Mesendoderm Formation and Left-Right Patterning.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Role of the Vg1 Zebrafish Ortholog GDF3 in Nodal-Dependent Mesendoderm Formation and Left-Right Patterning./
Author:	Pelliccia, Jose Luis.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2019,
Description:	100 p.
Notes:	Source: Dissertations Abstracts International, Volume: 80-09, Section: B.
Contained By:	Dissertations Abstracts International80-09B.
Subject:	Developmental biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13424783
ISBN:	9780438866911

The Role of the Vg1 Zebrafish Ortholog GDF3 in Nodal-Dependent Mesendoderm Formation and Left-Right Patterning.
Pelliccia, Jose Luis.

The Role of the Vg1 Zebrafish Ortholog GDF3 in Nodal-Dependent Mesendoderm Formation and Left-Right Patterning. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 100 p.

Source: Dissertations Abstracts International, Volume: 80-09, Section: B.

Thesis (Ph.D.)--Princeton University, 2019.

This item must not be sold to any third party vendors.

Nodal signaling plays an important role in mesendoderm formation and the placement of organs during vertebrate embryonic patterning. There are three Nodal (a TGFβ ligand) orthologs in zebrafish; the nodal orthologs squint (sqt or nodal-related ) and cyclops (cyc or nodal-related 2) are required for mesendoderm formation while the nodal ortholog southpaw (spaw) is required for the organization of organs. Here, I report on the role of another TGFβ ligand, the Vg1 ortholog in zebrafish called Growth differentiation factor 3 (Gdf3). Vg1 orthologs have been implicated in mesoderm formation and left-right patterning in the mouse and Xenopus. However, mesoderm defects had not been observed when Gdf3 was depleted in zebrafish embryos, which suggested the need for a more comprehensive investigation of Gdf3 function during zebrafish embryonic development. Using CRISPR/Cas9 generated mutants and Gdf3 knockdown techniques, we demonstrate that maternal gdf3 is required for Nodal-dependent mesendoderm formation. Furthermore, we show that Gdf3 is important for the formation of the left-right organizer and for Nodal signaling during left-right patterning. Our data suggest that Gdf3 is a facilitator of Nodal signaling. And although Nodal signaling can occur without Gdf3, the signaling potential is insufficient for proper mesendoderm formation and left-right patterning. Interestingly, our work also reveals that Gdf3 augments the function of the Nodal signaling inhibitor Lefty1. Collectively, our data indicate that Gdf3 potentiates the function of the Nodal ligand to promote robust Nodal signaling, while also enhancing the function of Lefty1 to negatively regulate signaling pathways during zebrafish embryonic development.

ISBN: 9780438866911Subjects--Topical Terms:

592588
Developmental biology.

The Role of the Vg1 Zebrafish Ortholog GDF3 in Nodal-Dependent Mesendoderm Formation and Left-Right Patterning.
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020 $a 9780438866911
035 $a (MiAaPQ)AAI13424783
035 $a (MiAaPQ)princeton:12851
035 $a AAI13424783
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100 1 $a Pelliccia, Jose Luis. $3 3437831
245 1 4 $a The Role of the Vg1 Zebrafish Ortholog GDF3 in Nodal-Dependent Mesendoderm Formation and Left-Right Patterning.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 100 p.
500 $a Source: Dissertations Abstracts International, Volume: 80-09, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
500 $a Advisor: Burdine, Rebecca D.
502 $a Thesis (Ph.D.)--Princeton University, 2019.
506 $a This item must not be sold to any third party vendors.
520 $a Nodal signaling plays an important role in mesendoderm formation and the placement of organs during vertebrate embryonic patterning. There are three Nodal (a TGFβ ligand) orthologs in zebrafish; the nodal orthologs squint (sqt or nodal-related ) and cyclops (cyc or nodal-related 2) are required for mesendoderm formation while the nodal ortholog southpaw (spaw) is required for the organization of organs. Here, I report on the role of another TGFβ ligand, the Vg1 ortholog in zebrafish called Growth differentiation factor 3 (Gdf3). Vg1 orthologs have been implicated in mesoderm formation and left-right patterning in the mouse and Xenopus. However, mesoderm defects had not been observed when Gdf3 was depleted in zebrafish embryos, which suggested the need for a more comprehensive investigation of Gdf3 function during zebrafish embryonic development. Using CRISPR/Cas9 generated mutants and Gdf3 knockdown techniques, we demonstrate that maternal gdf3 is required for Nodal-dependent mesendoderm formation. Furthermore, we show that Gdf3 is important for the formation of the left-right organizer and for Nodal signaling during left-right patterning. Our data suggest that Gdf3 is a facilitator of Nodal signaling. And although Nodal signaling can occur without Gdf3, the signaling potential is insufficient for proper mesendoderm formation and left-right patterning. Interestingly, our work also reveals that Gdf3 augments the function of the Nodal signaling inhibitor Lefty1. Collectively, our data indicate that Gdf3 potentiates the function of the Nodal ligand to promote robust Nodal signaling, while also enhancing the function of Lefty1 to negatively regulate signaling pathways during zebrafish embryonic development.
590 $a School code: 0181.
650 4 $a Developmental biology. $3 592588
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773 0 $t Dissertations Abstracts International $g 80-09B.
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793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13424783