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Functionalized Microarray Substrates...

Wu, Han.

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Functionalized Microarray Substrates for Chemical and Biological Applications.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Functionalized Microarray Substrates for Chemical and Biological Applications./
Author:	Wu, Han.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	142 p.
Notes:	Source: Dissertations Abstracts International, Volume: 80-06, Section: B.
Contained By:	Dissertations Abstracts International80-06B.
Subject:	Molecular biology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=11012260
ISBN:	9780438659629

Functionalized Microarray Substrates for Chemical and Biological Applications.
Wu, Han.

Functionalized Microarray Substrates for Chemical and Biological Applications. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 142 p.

Source: Dissertations Abstracts International, Volume: 80-06, Section: B.

Thesis (Ph.D.)--The Chinese University of Hong Kong (Hong Kong), 2018.

This item must not be sold to any third party vendors.

This thesis describes the fabrication of two kinds of functionalized microarray substrates. Firstly, a hydrophilic microarray on the hydrophobic surface was created by negative microcontact printing. The hydrophilic-hydrophobic platform was used in chemical and biological patterning studies. Secondly, a rehydratable gel-in-microwell array was fabricated by synthesizing small hydrogel particles in the glass microwells. The gel-in-microwell array was used for biomolecules detection. In the first part, a negative microcontact printing method was developed to fabricate hydrophilic polydopamine (PDA) arrays with micrometer resolution on hydrophobic surfaces. Unlike the conventional microcontact printing which prints molecules onto substrates, in the negative microcontact printing, the molecules were removed at the contact area by polydimethylsiloxane (PDMS) stamp. The negative microcontact printing started with the formation of a PDA thin film on the hydrophobic surface, followed by the contact of a plasma activated PDMS stamp. Taking advantage of the difference of the surface energy between the hydrophobic surface and the plasma activated PDMS stamp, PDA was removed from the hydrophobic surface at the contact area, leaving behind a complementary pattern to the PDMS stamp on the surface. With the hydrophilic PDA arrays on the hydrophobic substrate, arrays of single stem cells were further obtained by exploiting the different cell adhesion behaviors on the hydrophilic and the hydrophobic surfaces and by controlling the size of the PDA spots on the hydrophobic surface. In the second part, a focal length tunable microlens array was developed on a hydrophilic-hydrophobic substrate. Based on the negative microcontact printing method, hydrophilic PDA patterns on the perfluorinated CYTOP™ surface were facilely generated. With the hydrophilic-hydrophobic substrate, droplet array was first formed by discontinuous dewetting. Droplet arrays of both aqueous solutions and low-surface-tension liquids were generated on the substrate in a single step, allowing the fabrication of microlenses with various materials. The obtained droplet array was then photo-polymerized to form polymer micropad array on the glass substrate. The planar substrate with convex shaped micropads showed a great potential for acting as microlenses. The focal length of the microlens array was further controlled by changing the compositions of the polymer micropads, resulting in a smart microlens array with tunable imaging properties. In the third part, we developed a rehydratable gel-in-microwell array platform for user-friendly and cost-saving digital polymerase chain reaction (dPCR). Thousands of microwells with the volume of picoliter were fabricated on the glass substrate by soft lithography and wet etching. DNA amplification occurred in hydrogel particles synthesized in the microwells. Rehydratable hydrogel provided an excellent platform for convenient and effective long time storage of PCR components. PCR mixtures were preloaded into the hydrogel particles except the templates. For quantitative DNA detection, we only needed to add the template solution onto the dehydrated hydrogel platform to rehydrate the hydrogel. A Peltier element and a CCD camera were used to perform the PCR thermal cycling and capture the fluorescence from the PCR product. The rehydratable gel-in-microwell array platform is amenable for point-of-care biomolecules detection.

ISBN: 9780438659629Subjects--Topical Terms:

517296
Molecular biology.

Functionalized Microarray Substrates for Chemical and Biological Applications.
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245 1 0 $a Functionalized Microarray Substrates for Chemical and Biological Applications.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2018
300 $a 142 p.
500 $a Source: Dissertations Abstracts International, Volume: 80-06, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
502 $a Thesis (Ph.D.)--The Chinese University of Hong Kong (Hong Kong), 2018.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
520 $a This thesis describes the fabrication of two kinds of functionalized microarray substrates. Firstly, a hydrophilic microarray on the hydrophobic surface was created by negative microcontact printing. The hydrophilic-hydrophobic platform was used in chemical and biological patterning studies. Secondly, a rehydratable gel-in-microwell array was fabricated by synthesizing small hydrogel particles in the glass microwells. The gel-in-microwell array was used for biomolecules detection. In the first part, a negative microcontact printing method was developed to fabricate hydrophilic polydopamine (PDA) arrays with micrometer resolution on hydrophobic surfaces. Unlike the conventional microcontact printing which prints molecules onto substrates, in the negative microcontact printing, the molecules were removed at the contact area by polydimethylsiloxane (PDMS) stamp. The negative microcontact printing started with the formation of a PDA thin film on the hydrophobic surface, followed by the contact of a plasma activated PDMS stamp. Taking advantage of the difference of the surface energy between the hydrophobic surface and the plasma activated PDMS stamp, PDA was removed from the hydrophobic surface at the contact area, leaving behind a complementary pattern to the PDMS stamp on the surface. With the hydrophilic PDA arrays on the hydrophobic substrate, arrays of single stem cells were further obtained by exploiting the different cell adhesion behaviors on the hydrophilic and the hydrophobic surfaces and by controlling the size of the PDA spots on the hydrophobic surface. In the second part, a focal length tunable microlens array was developed on a hydrophilic-hydrophobic substrate. Based on the negative microcontact printing method, hydrophilic PDA patterns on the perfluorinated CYTOP™ surface were facilely generated. With the hydrophilic-hydrophobic substrate, droplet array was first formed by discontinuous dewetting. Droplet arrays of both aqueous solutions and low-surface-tension liquids were generated on the substrate in a single step, allowing the fabrication of microlenses with various materials. The obtained droplet array was then photo-polymerized to form polymer micropad array on the glass substrate. The planar substrate with convex shaped micropads showed a great potential for acting as microlenses. The focal length of the microlens array was further controlled by changing the compositions of the polymer micropads, resulting in a smart microlens array with tunable imaging properties. In the third part, we developed a rehydratable gel-in-microwell array platform for user-friendly and cost-saving digital polymerase chain reaction (dPCR). Thousands of microwells with the volume of picoliter were fabricated on the glass substrate by soft lithography and wet etching. DNA amplification occurred in hydrogel particles synthesized in the microwells. Rehydratable hydrogel provided an excellent platform for convenient and effective long time storage of PCR components. PCR mixtures were preloaded into the hydrogel particles except the templates. For quantitative DNA detection, we only needed to add the template solution onto the dehydrated hydrogel platform to rehydrate the hydrogel. A Peltier element and a CCD camera were used to perform the PCR thermal cycling and capture the fluorescence from the PCR product. The rehydratable gel-in-microwell array platform is amenable for point-of-care biomolecules detection.
590 $a School code: 1307.
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710 2 $a The Chinese University of Hong Kong (Hong Kong). $b Chemistry. $3 3435015
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