東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Study on the Molecular Regulation of...

Wang, Guanqun.

Linked to FindBook

Google Book

Amazon

博客來

Study on the Molecular Regulation of Remobilization of Carbon Reserve from Stems to Grains and the Role of Trehalose-6-phosphate Phosphatase in Seed Germination in Rice.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Study on the Molecular Regulation of Remobilization of Carbon Reserve from Stems to Grains and the Role of Trehalose-6-phosphate Phosphatase in Seed Germination in Rice./
Author:	Wang, Guanqun.
Published:	Ann Arbor : ProQuest Dissertations & Theses, : 2018,
Description:	133 p.
Notes:	Source: Dissertations Abstracts International, Volume: 79-12, Section: B.
Contained By:	Dissertations Abstracts International79-12B.
Subject:	Botany. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10902205
ISBN:	9780438106314

Study on the Molecular Regulation of Remobilization of Carbon Reserve from Stems to Grains and the Role of Trehalose-6-phosphate Phosphatase in Seed Germination in Rice.
Wang, Guanqun.

Study on the Molecular Regulation of Remobilization of Carbon Reserve from Stems to Grains and the Role of Trehalose-6-phosphate Phosphatase in Seed Germination in Rice. - Ann Arbor : ProQuest Dissertations & Theses, 2018 - 133 p.

Source: Dissertations Abstracts International, Volume: 79-12, Section: B.

Thesis (Ph.D.)--The Chinese University of Hong Kong (Hong Kong), 2018.

This item must not be sold to any third party vendors.

Carbon reserves in rice stems (culm and sheath) before flowering contribute to a significant portion of grain filling. In this study, super rice LYP9 and conventional rice 9311 showed different carbon reserve remobilization behaviors. The transcriptomic profiles of stems of LYP9 and 9311 were analyzed at three stages of grain filling. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene Ontology (GO) classification of DGs both shown that carbohydrate catabolic pathway, plant hormone signal transduction and photosynthesis pathway were DGs-enriched, suggesting their roles in carbon reserve remobilization, which somewhat explain the difference of NSC content, photosynthesis and ABA content between the two cultivars during grain filling. Further analysis suggest that genes involved in trehalose synthesis (TPP, TPS), starch degradation (beta-amylase) and sucrose synthesis (SPS, SS) were important for carbon reserves remobilization whereas ABA content was determined by counteraction of NCED1 and ABA8ox1 gene. The higher expression level of all these genes and ABA content in 9311 resulted in better efficiency of carbon reserves remobilization in 9311 than cultivar of LYP9. Moderate soil drying (MD) imposed at post-anthesis stages can increase starch accumulation in inferior grains. Based on the accumulation dynamics of soluble sugars and starch in inferior grains under well-watered (CK) and MD treatments at postanthesis in the YD cultivar, RNA-seq analysis of the inferior grains was carried out. We found that the elevated ABA level induced by MD was consistent with the downregulation of the ABA8ox2 gene, which belongs to the ABA catabolism family, suggesting that the lower expression level of ABA8ox2 might be responsible for a higher ABA content, potentially resulting in better grain filling in inferior grains. Furthermore, the MD treatment altered the expression pattern of genes related to ABA signal transduction, which might participate in the process of starch accumulation in the inferior grains. Besides, genes encoding five key enzymes involved in the conversion of sucrose to starch were up-regulated, which are responsible for soluble sugar reduction and starch accumulation in inferior grains under MD. Furthermore, the ABA8ox2 -mediated increase in ABA levels might be involved in the process of inferior grain filling under MD. The trehalose-6-phosphate phosphatase 1 (TPP1) gene, which is reported to play roles in improving yield in maize and stress tolerance in rice, also promoted seed germination. tpp1 mutant seeds germinated significantly more slowly than ZH11 seeds. The ABA content of dry tpp1 seeds was higher than that of ZH11 seeds. In addition, tpp1 seeds were hypersensitive to ABA and an ABA catabolism inhibitor (diniconazole). Both exogenous ABA and diniconazole also inhibited the expression of the OsTPP1 gene in seeds. Further analysis showed that the tpp1 mutant was sensitive to the gibberellin (GA) biosynthesis inhibitor paclobutrazol (PAC). The expression levels of the hormone genes ABA 8'-hydroxylase 3 (OsABA8ox3) and GA 2-oxidase 1 (OsGA2ox1) were increased, while the GA 20-oxidase 1 (OsGA20ox1) was reduced in the tpp1 mutant during seed germination. Overall, TPP1-regulated seed germination was found to be mediated by ABA catabolism, GA synthesis and GA catabolism in imbibed seeds.

ISBN: 9780438106314Subjects--Topical Terms:

516217
Botany.

Study on the Molecular Regulation of Remobilization of Carbon Reserve from Stems to Grains and the Role of Trehalose-6-phosphate Phosphatase in Seed Germination in Rice.
LDR:04665nmm a2200337 4500 001 2207956
005 20190929184018.5
008 201008s2018 ||||||||||||||||| ||eng d
020 $a 9780438106314
035 $a (MiAaPQ)AAI10902205
035 $a AAI10902205
040 $a MiAaPQ $c MiAaPQ
100 1 $a Wang, Guanqun. $3 3434955
245 1 0 $a Study on the Molecular Regulation of Remobilization of Carbon Reserve from Stems to Grains and the Role of Trehalose-6-phosphate Phosphatase in Seed Germination in Rice.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2018
300 $a 133 p.
500 $a Source: Dissertations Abstracts International, Volume: 79-12, Section: B.
500 $a Publisher info.: Dissertation/Thesis.
502 $a Thesis (Ph.D.)--The Chinese University of Hong Kong (Hong Kong), 2018.
506 $a This item must not be sold to any third party vendors.
506 $a This item must not be added to any third party search indexes.
506 $a This item is not available from ProQuest Dissertations & Theses.
520 $a Carbon reserves in rice stems (culm and sheath) before flowering contribute to a significant portion of grain filling. In this study, super rice LYP9 and conventional rice 9311 showed different carbon reserve remobilization behaviors. The transcriptomic profiles of stems of LYP9 and 9311 were analyzed at three stages of grain filling. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene Ontology (GO) classification of DGs both shown that carbohydrate catabolic pathway, plant hormone signal transduction and photosynthesis pathway were DGs-enriched, suggesting their roles in carbon reserve remobilization, which somewhat explain the difference of NSC content, photosynthesis and ABA content between the two cultivars during grain filling. Further analysis suggest that genes involved in trehalose synthesis (TPP, TPS), starch degradation (beta-amylase) and sucrose synthesis (SPS, SS) were important for carbon reserves remobilization whereas ABA content was determined by counteraction of NCED1 and ABA8ox1 gene. The higher expression level of all these genes and ABA content in 9311 resulted in better efficiency of carbon reserves remobilization in 9311 than cultivar of LYP9. Moderate soil drying (MD) imposed at post-anthesis stages can increase starch accumulation in inferior grains. Based on the accumulation dynamics of soluble sugars and starch in inferior grains under well-watered (CK) and MD treatments at postanthesis in the YD cultivar, RNA-seq analysis of the inferior grains was carried out. We found that the elevated ABA level induced by MD was consistent with the downregulation of the ABA8ox2 gene, which belongs to the ABA catabolism family, suggesting that the lower expression level of ABA8ox2 might be responsible for a higher ABA content, potentially resulting in better grain filling in inferior grains. Furthermore, the MD treatment altered the expression pattern of genes related to ABA signal transduction, which might participate in the process of starch accumulation in the inferior grains. Besides, genes encoding five key enzymes involved in the conversion of sucrose to starch were up-regulated, which are responsible for soluble sugar reduction and starch accumulation in inferior grains under MD. Furthermore, the ABA8ox2 -mediated increase in ABA levels might be involved in the process of inferior grain filling under MD. The trehalose-6-phosphate phosphatase 1 (TPP1) gene, which is reported to play roles in improving yield in maize and stress tolerance in rice, also promoted seed germination. tpp1 mutant seeds germinated significantly more slowly than ZH11 seeds. The ABA content of dry tpp1 seeds was higher than that of ZH11 seeds. In addition, tpp1 seeds were hypersensitive to ABA and an ABA catabolism inhibitor (diniconazole). Both exogenous ABA and diniconazole also inhibited the expression of the OsTPP1 gene in seeds. Further analysis showed that the tpp1 mutant was sensitive to the gibberellin (GA) biosynthesis inhibitor paclobutrazol (PAC). The expression levels of the hormone genes ABA 8'-hydroxylase 3 (OsABA8ox3) and GA 2-oxidase 1 (OsGA2ox1) were increased, while the GA 20-oxidase 1 (OsGA20ox1) was reduced in the tpp1 mutant during seed germination. Overall, TPP1-regulated seed germination was found to be mediated by ABA catabolism, GA synthesis and GA catabolism in imbibed seeds.
590 $a School code: 1307.
650 4 $a Botany. $3 516217
650 4 $a Bioinformatics. $3 553671
650 4 $a Physiology. $3 518431
690 $a 0309
690 $a 0715
690 $a 0719
710 2 $a The Chinese University of Hong Kong (Hong Kong). $b Biology. $3 3434956
773 0 $t Dissertations Abstracts International $g 79-12B.
790 $a 1307
791 $a Ph.D.
792 $a 2018
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=10902205